Human ROR alpha /NR1F1 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human ROR alpha /NR1F1 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 784652
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human ROR alpha /NR1F1
    Accession # P35398
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of ROR alpha /NR1F1 in Th17 Differentiated Human PBMCs by Flow Cytometry. Th17 differentiated human peripheral blood mononuclear cells (PBMCs, panel A) or resting PBMCs (panel B) were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and Mouse Anti-Human ROR alpha /NR1F1 PE‑conjugated Monoclonal Antibody (Catalog # IC8924P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROR alpha/NR1F1
RORa (Retinoid-related Orphan Receptor alpha ) is a member of the NR1 nuclear hormone receptor family. ROR alpha is a DNA binding transcription factor, and can bind as a monomer or homodimer to hormone response elements to enhance expression.  Knockout mice implicate ROR alpha as being essential in the development of the cerebellum. It has also been reported to regulate lymphocyte development and play a role in Th17 differentiation. Human ROR alpha shares 99% amino sequence identity with mouse ROR alpha.
  • Long Name:
    Retinoic Acid-Related Orphan Receptor alpha
  • Entrez Gene IDs:
    6095 (Human)
  • Alternate Names:
    DKFZp686M2414; MGC119326; NR1F1; NR1F1MGC119329; nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR-related orphan receptor A; RAR-related orphan receptor alpha; retinoic acid receptor-related orphan receptor alpha; retinoid-related orphan receptor alpha; Retinoid-related orphan receptor-alpha; ROR1; ROR2; ROR3; RORA; RZRA; RZR-ALPHA; RZRAROR-alpha; transcription factor RZR-alpha
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 12  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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