Human ROR alpha /NR1F1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC8924P-025
IC8924P-100
Detection of ROR alpha /NR1F1 in Th17 Differentiated Human PBMCs by Flow Cytometry.
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Human ROR alpha /NR1F1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ROR alpha /NR1F1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 784652
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ROR alpha /NR1F1
Arg47-His163
Accession # P35398
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of ROR alpha /NR1F1 in Th17 Differentiated Human PBMCs by Flow Cytometry. View Larger

Detection of ROR alpha /NR1F1 in Th17 Differentiated Human PBMCs by Flow Cytometry. Th17 differentiated human peripheral blood mononuclear cells (PBMCs, panel A) or resting PBMCs (panel B) were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and Mouse Anti-Human ROR alpha /NR1F1 PE‑conjugated Monoclonal Antibody (Catalog # IC8924P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ROR alpha/NR1F1

RORa (Retinoid-related Orphan Receptor alpha ) is a member of the NR1 nuclear hormone receptor family. ROR alpha is a DNA binding transcription factor, and can bind as a monomer or homodimer to hormone response elements to enhance expression.  Knockout mice implicate ROR alpha as being essential in the development of the cerebellum. It has also been reported to regulate lymphocyte development and play a role in Th17 differentiation. Human ROR alpha shares 99% amino sequence identity with mouse ROR alpha.

Long Name
Retinoic Acid-Related Orphan Receptor alpha
Entrez Gene IDs
6095 (Human)
Alternate Names
DKFZp686M2414; MGC119326; NR1F1; NR1F1MGC119329; nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR-related orphan receptor A; RAR-related orphan receptor alpha; retinoic acid receptor-related orphan receptor alpha; retinoid-related orphan receptor alpha; Retinoid-related orphan receptor-alpha; ROR alpha; ROR1; ROR2; ROR3; RORA; RZRA; RZR-ALPHA; RZRAROR-alpha; transcription factor RZR-alpha

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