Human ROR alpha /NR1F1 Antibody

Catalog # Availability Size / Price Qty
MAB8924
MAB8924-SP
Detection of ROR alpha /NR1F1 in Human PBMCs by Flow Cytometry.
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Human ROR alpha /NR1F1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ROR alpha /NR1F1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 784652
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ROR alpha /NR1F1
Arg47-His163
Accession # P35398
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of ROR alpha /NR1F1 in Human PBMCs by Flow Cytometry. View Larger

Detection of ROR alpha /NR1F1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th17 cells were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human ROR alpha /NR1F1 Monoclonal Antibody (Catalog # MAB8924) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR alpha/NR1F1

RORa (Retinoid-related Orphan Receptor alpha ) is a member of the NR1 nuclear hormone receptor family. ROR alpha is a DNA binding transcription factor, and can bind as a monomer or homodimer to hormone response elements to enhance expression.  Knockout mice implicate ROR alpha as being essential in the development of the cerebellum. It has also been reported to regulate lymphocyte development and play a role in Th17 differentiation. Human ROR alpha shares 99% amino sequence identity with mouse ROR alpha.

Long Name
Retinoic Acid-Related Orphan Receptor alpha
Entrez Gene IDs
6095 (Human)
Alternate Names
DKFZp686M2414; MGC119326; NR1F1; NR1F1MGC119329; nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR-related orphan receptor A; RAR-related orphan receptor alpha; retinoic acid receptor-related orphan receptor alpha; retinoid-related orphan receptor alpha; Retinoid-related orphan receptor-alpha; ROR alpha; ROR1; ROR2; ROR3; RORA; RZRA; RZR-ALPHA; RZRAROR-alpha; transcription factor RZR-alpha

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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