Human RSK4 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human RSK4 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) RSK1, rhRSK2, or rhRSK3 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 374521
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human RSK4
    Met1-Leu745
    Accession # Q9UK32
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human RSK4 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL Rat Anti-Human RSK4 Monoclonal Antibody (Catalog # MAB3916) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for RSK4 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human RSK4 by Western Blot.

Western blot shows Recombinant Human Active RSK1 (Catalog # 992-KS), recombinant human RSK2, recombinant human RSK3, and recombinant human RSK4 (2 ng/lane). PVDF membrane was probed with 2 µg/mL Rat Anti-Human RSK4 Monoclonal Antibody (Catalog # MAB3916) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for RSK4 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RSK4

The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases frequently activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSK4, also known as RPS6KA6, is commonly deleted in patients with complex X-linked mental retardation. Unlike other RSKs, RSK4 is constitutively active in serum-starved cells, and may participate in non-growth factor signaling.

  • Long Name:
    Ribosomal Protein S6 Kinase IV
  • Entrez Gene IDs:
    27330 (Human); 67071 (Mouse); 317203 (Rat)
  • Alternate Names:
    EC 2.7.11; EC 2.7.11.1; p90-RSK 6; p90RSK6; pp90RSK4; PP90RSK4,90 kDa ribosomal protein S6 kinase 6; ribosomal protein S6 kinase alpha-6; ribosomal protein S6 kinase, 90kDa, polypeptide 6; Ribosomal S6 kinase 4; RPS6KA6; RSK4; RSK-4; RSK4ribosomal protein S6 kinase, 90kD, polypeptide 6; S6K-alpha 6; S6K-alpha-6
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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