Human RUNX2/CBFA1 Biotinylated Antibody

Catalog #: BAF2006 Datasheet / COA / SDS

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BAF2006

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RUNX2/CBFA1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CBFA1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human RUNX2 isoform 2
Lys219-Tyr404
Accession # Q13950-2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human RUNX2/CBFA1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RUNX2/CBFA1

Core Binding Factor A1 (CBFA1; also called AML-3, PEBP-2 alpha A, OSF-2, RUNX2) is a 56 kDa DNA-binding protein that belongs to the Runt-related family of transcription factors. It is synthesized as a 521 amino acid (aa) protein with multiple domains. From the N-terminus, there is a 19 aa activation domain (AD1), a 41 aa glutamine/alanine (Q/A) repeat segment (AD2), a 128 aa Runt/DNA-binding region, a 9 aa NLS and a 285 aa ProSerThr (PST)-rich sequence that is needed for gene activation. Two isoforms exist, one with a truncated AD1 domain and another with a 22 aa deletion in the AD3 section of PST-rich sequence. Human CBFA1 is 98% aa identical to mouse CBFA1.

Long Name

Runt-related Transcription Factor 2

Entrez Gene IDs

860 (Human)

Alternate Names

Acute myeloid leukemia 3 protein; CBFA1; CBF-alpha-1; CCD1; CCDAML3; CLCD; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; MGC120023; ML3; oncogene AML-3; OSF2; OSF-2; osteoblast-specific transcription factor 2; PEA2aA; PEA2-alpha A; PEBP2A; PEBP2aA; PEBP2-alpha A; polyomavirus enhancer-binding protein 2 alpha A subunit; runt domain, alpha subunit 1; runt related transcription factor 2; runt-related transcription factor 2; RUNX2; SL3/AKV core-binding factor alpha A subunit; SL3-3 enhancer factor 1 alpha A subunit