Human RUNX2/CBFA1 Antibody
Human RUNX2/CBFA1 Antibody Summary
Lys219-Tyr404
Accession # Q13950
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of RUNX2/CBFA1-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. RUNX2/CBFA1/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human RUNX2/CBFA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2006) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. TheVEGFpromoter was detected by standard PCR.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RUNX2/CBFA1
Core Binding Factor A1 (CBFA1; also called AML-3, PEBP-2 alpha A, OSF-2, RUNX2) is a 56 kDa DNA-binding protein that belongs to the Runt-related family of transcription factors. It is synthesized as a 521 amino acid (aa) protein with multiple domains. From the N-terminus, there is a 19 aa activation domain (AD1), a 41 aa glutamine/alanine (Q/A) repeat segment (AD2), a 128 aa Runt/DNA-binding region, a 9 aa NLS and a 285 aa ProSerThr (PST)-rich sequence that is needed for gene activation. Two isoforms exist, one with a truncated AD1 domain and another with a 22 aa deletion in the AD3 section of PST-rich sequence. Human CBFA1 is 98% aa identical to mouse CBFA1.
Product Datasheets
Citations for Human RUNX2/CBFA1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Physiologic Mechanical Stress Directly Induces Bone Formation by Activating Glucose Transporter 1 (Glut 1) in Osteoblasts, Inducing Signaling via NAD+-Dependent Deacetylase (Sirtuin 1) and Runt-Related Transcription Factor 2 (Runx2)
Authors: S Somemura, T Kumai, K Yatabe, C Sasaki, H Fujiya, H Niki, K Yudoh
International Journal of Molecular Sciences, 2021;22(16):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Pleiotropic Roles of NOTCH1 Signaling in the Loss of Maturational Arrest of Human Osteoarthritic Chondrocytes
Authors: M Minguzzi, V Panichi, S D'Adamo, S Cetrullo, L Cattini, F Flamigni, E Mariani, RM Borzì
International Journal of Molecular Sciences, 2021;22(21):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Histone Deacetylase Inhibitors Repress Tumoral Expression of the Proinvasive Factor RUNX2.
Authors: Sancisi V, Gandolfi G, Ambrosetti D, Ciarrocchi A
Cancer Res, 2015;75(9):1868-82.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Differential requirements for IKKalpha and IKKbeta in the differentiation of primary human osteoarthritic chondrocytes.
Authors: Olivotto E, Borzi RM, Vitellozzi R, Pagani S, Facchini A, Battistelli M, Penzo M, Li X, Flamigni F, Li J, Falcieri E, Facchini A, Marcu KB
Arthritis Rheum., 2008;58(1):227-39.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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