Human RUNX2/CBFA1 Antibody

Catalog # Availability Size / Price Qty
Detection of RUNX2/CBFA1-regulated Genes by Chromatin Immunoprecipitation.
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Product Details
Citations (4)
Supplemental Products

Human RUNX2/CBFA1 Antibody Summary

Species Reactivity
Detects human RUNX2/CBFA1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) RUNX1 and rhRUNX3 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human RUNX2/CBFA1 isoform 2
Accession # Q13950
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human RUNX2/CBFA1
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
5-15 µg/mL
Immersion fixed MC3T3-E1 mouse preosteoblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Chromatin Immunoprecipitation (ChIP) Detection of RUNX2/CBFA1-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of RUNX2/CBFA1-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. RUNX2/CBFA1/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human RUNX2/CBFA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2006) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. TheVEGFpromoter was detected by standard PCR.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RUNX2/CBFA1

Core Binding Factor A1 (CBFA1; also called AML-3, PEBP-2 alpha A, OSF-2, RUNX2) is a 56 kDa DNA-binding protein that belongs to the Runt-related family of transcription factors. It is synthesized as a 521 amino acid (aa) protein with multiple domains. From the N-terminus, there is a 19 aa activation domain (AD1), a 41 aa glutamine/alanine (Q/A) repeat segment (AD2), a 128 aa Runt/DNA-binding region, a 9 aa NLS and a 285 aa ProSerThr (PST)-rich sequence that is needed for gene activation. Two isoforms exist, one with a truncated AD1 domain and another with a 22 aa deletion in the AD3 section of PST-rich sequence. Human CBFA1 is 98% aa identical to mouse CBFA1.

Long Name
Runt-related Transcription Factor 2
Entrez Gene IDs
860 (Human)
Alternate Names
Acute myeloid leukemia 3 protein; CBFA1; CBF-alpha-1; CCD1; CCDAML3; CLCD; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; MGC120023; ML3; oncogene AML-3; OSF2; OSF-2; osteoblast-specific transcription factor 2; PEA2aA; PEA2-alpha A; PEBP2A; PEBP2aA; PEBP2-alpha A; polyomavirus enhancer-binding protein 2 alpha A subunit; runt domain, alpha subunit 1; runt related transcription factor 2; runt-related transcription factor 2; RUNX2; SL3/AKV core-binding factor alpha A subunit; SL3-3 enhancer factor 1 alpha A subunit

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Citations for Human RUNX2/CBFA1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Physiologic Mechanical Stress Directly Induces Bone Formation by Activating Glucose Transporter 1 (Glut 1) in Osteoblasts, Inducing Signaling via NAD+-Dependent Deacetylase (Sirtuin 1) and Runt-Related Transcription Factor 2 (Runx2)
    Authors: S Somemura, T Kumai, K Yatabe, C Sasaki, H Fujiya, H Niki, K Yudoh
    International Journal of Molecular Sciences, 2021;22(16):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Pleiotropic Roles of NOTCH1 Signaling in the Loss of Maturational Arrest of Human Osteoarthritic Chondrocytes
    Authors: M Minguzzi, V Panichi, S D'Adamo, S Cetrullo, L Cattini, F Flamigni, E Mariani, RM Borzì
    International Journal of Molecular Sciences, 2021;22(21):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Histone Deacetylase Inhibitors Repress Tumoral Expression of the Proinvasive Factor RUNX2.
    Authors: Sancisi V, Gandolfi G, Ambrosetti D, Ciarrocchi A
    Cancer Res, 2015;75(9):1868-82.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Differential requirements for IKKalpha and IKKbeta in the differentiation of primary human osteoarthritic chondrocytes.
    Authors: Olivotto E, Borzi RM, Vitellozzi R, Pagani S, Facchini A, Battistelli M, Penzo M, Li X, Flamigni F, Li J, Falcieri E, Facchini A, Marcu KB
    Arthritis Rheum., 2008;58(1):227-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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