Human S100A9 DuoSet ELISA
Human S100A9 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
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Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: S100A9
Human S100A9 (also MRP-14, Calgranulin-B, and p14) is a 14 kDa member of the S100 family of EF-hand calcium-binding proteins. It is 114 amino acids (aa) in length and contains short sequential modules. There is an N-terminal helical region, followed by a calcium-binding EF-hand domain, two more helical regions, a second EF-hand domain, and three additional helical regions. S100A9 will noncovalently heterodimerize with S100A8. In the presence of calcium, this heterodimer will form a heterotetramer. S100A9 is expressed in granulocytes, monocytes, and macrophages during acute and chronic inflammation. Human S100A9 shares 62% and 57% aa identity with rat and mouse S100A9, respectively.
Citations for Human S100A9 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism
Authors: C Monteiro, L Miarka, M Perea-Garc, N Priego, P García-Góm, L Álvaro-Esp, A de Pablos-, N Yebra, D Retana, P Baena, C Fustero-To, O Graña-Cast, K Troulé, E Caleiras, P Tezanos, P Muela, E Cintado, JL Trejo, JM Sepúlveda, P González-L, L Jiménez-Ro, LM Moreno, O Esteban, Á Pérez-Núñe, A Hernández-, J Mazarico G, I Ferrer, R Suárez, EM Garrido-Ma, L Paz-Ares, C Dalmasso, E Cohen-Jona, A Siegfried, A Hegarty, S Keelan, D Varešlija, LS Young, M Mohme, Y Goy, H Wikman, J Fernández-, G Blasco, L Alcázar, C Cabañuz, SI Grivenniko, A Ianus, N Shemesh, CC Faria, R Lee, P Lorigan, E Le Rhun, M Weller, R Soffietti, L Bertero, U Ricardi, J Bosch-Barr, E Sais, E Teixidor, A Hernández-, A Calvo, J Aristu, SM Martin, A Gonzalez, O Adler, N Erez, RENACER, M Valiente
Nature Medicine, 2022;28(4):752-765.
Species: Human
Sample Types: Cell Culture Supernates
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Using tears as a non-invasive source for early detection of breast cancer
Authors: A Daily, P Ravishanka, S Harms, VS Klimberg
PLoS ONE, 2022;17(4):e0267676.
Species: Human
Sample Types: Tears
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Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation
Authors: H Xiang, F Guo, X Tao, Q Zhou, S Xia, D Deng, L Li, D Shang
Theranostics, 2021;11(9):4467-4482.
Species: Human
Sample Types: Cell Culture Supernates
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Clinical Response to the CD95-Ligand Inhibitor Asunercept Is Defined by a Pro-Inflammatory Serum Cytokine Profile
Authors: A Radujkovic, T Boch, F Nolte, D Nowak, C Kunz, A Gieffers, C Müller-Tid, P Dreger, WK Hofmann, T Luft
Cancers, 2020;12(12):.
Species: Human
Sample Types: Serum
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S100A9 promotes prostate cancer cell invasion by activating TLR4/NF-&kappaB/integrin &beta1/FAK signaling
Authors: Z Lv, W Li, X Wei
OncoTargets and Therapy, 2020;13(0):6443-6452.
Species: Human
Sample Types: Cell Culture Supernates
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Ubiquitination of Listeria Virulence Factor InlC Contributes to the Host Response to Infection
Authors: E Gouin, D Balestrino, O Rasid, MA Nahori, V Villiers, F Impens, S Volant, T Vogl, Y Jacob, O Dussurget, P Cossart
MBio, 2019;10(6):.
Species: Human
Sample Types: Cell Culture Supernates
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Loss of the Immune Checkpoint CD85j/LILRB1 on Malignant Plasma Cells Contributes to Immune Escape in Multiple Myeloma.
Authors: E Lozano, Lozano E, Diaz T, T Díaz, MP Mena, Mena M, Sune G, G Suñe, X Calvo, M Calderón, Calderon M, Perez-Amill L, L Pérez-Amil, V Rodríguez, P Pérez-Galá, G Roué, MT Cibeira, Cibeira M, Rosinol L, L Rosiñol, I Isola, Isola I, Rodriguez-Lobato L, LG Rodríguez-, B Martin-Ant, J Bladé, Blade J, C Fernández
J Immunol, 0;200(8):2581-2591.
Species: Human
Sample Types: Plasma
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