Human Semaphorin 4A Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Semaphorin 4A in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse Semaphorin 4A is observed, and less than 1% cross-reactivity with recombinant human (rh) Semaphorin 4B, rhSemaphorin 4C, and rhSemaphorin 4D is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4A
    Gly32-His683
    Accession # Q9H3S1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Semaphorin 4A by Western Blot. Western blot shows lysates of human mature dendritic cells. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Semaphorin 4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4694) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 4A at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 4A

Sema4A (Semaphorin 4A; also Sema B) is a class IV member of the semaphorin family of proteins. It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells. It reportedly binds to Tim-2 and Plexin D1 and B1. In the immune system, it serves to activate naïve T cells and induce a Th1 phenotype. In the nervous system, it induces growth cone collapse in neurons. Mature human Sema4A is an 80 kDa (predicted) type I transmembrane glycoprotein that is 729 amino acids (aa) in length (aa 33-761). It contains a 651 aa extracellular region (aa 33-683) that is characterized by the presence of one Sema domain (aa 36-494), a PSI region (aa 496-528), and an Ig-like C2-type domain (aa 573-631). The 57 aa cytoplasmic region contains two potential phosphorylation sites at Thr744 and Ser747. There are two potential splice variants. One contains a 37 aa substitution for aa 293-328, while a second contains an alternative start site at Met100 coupled to a deletion of aa 122-154. Sema4A is reportedly cleaved to generate both a monomeric and a  dimeric soluble isoforms. Over aa 32-683, human Sema4A shares 86% aa identity with mouse Sema4A.

  • Entrez Gene IDs:
    64218 (Human); 20351 (Mouse); 310630 (Rat)
  • Alternate Names:
    CORD10; RP35; Sema B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4A; SEMA4A; SEMAB; Semaphorin 4A; semaphorin-4A; semaphorin-B; SEMB; SEMBFLJ12287
Related Research Areas
Isotype Controls
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Secondary Antibodies
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Donkey Anti-Sheep IgG Biotinylated Antibody

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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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