Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

ELISA Detection

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 660016
View all Semaphorin 4C Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4C
Ala21-Gly663 (predicted)
Accession # Q9C0C4

Specificity

Detects human Semaphorin 4C in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 3C, 3D, 3E, 3F, 4A, 4B, 4D, 4G, 5A, 5B, 6A, 6B, 6C, 6D, 7A, recombinant mouse Semaphorin 3B, 3C, 3E, 3F, 4A, 4B, 4C, 4D, 4F, 4G, 6A, 6B, 6C, 6D, or 7A is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Semaphorin 4C Antibody

Detection of Human Semaphorin 4C antibody by Western Blot.

Detection of Human Semaphorin 4C by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human Semaphorin 4C Monoclonal Antibody (Catalog # MAB6125) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Semaphorin 4C at approximately 100 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Semaphorin 4C Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 4C

Semaphorin 4C (Sema4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins.  It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms.  Mature human Semaphorin 4C is a type I transmembrane glycoprotein that is 813 amino acids (aa) in length.  It contains a 643 aa extracellular region (aa 21-663) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499-551), and an Ig-like C2-type domain (aa 556-644).  The cytoplasmic region interacts with PZD-domain containing proteins.  There are three potential splice variants.  One demonstrates an alternative start site at Met324, a second shows a deletion of aa 173-211, and a third contains an 89 aa substitution for aa 1-36.  Over aa 21-663, human Semaphorin 4C shares 85% aa identity with mouse Semaphorin 4C.                       

Alternate Names

SEMA4C, SEMACL1, Semaf, SEMAI

Entrez Gene IDs

54910 (Human); 20353 (Mouse); 301346 (Rat)

Gene Symbol

SEMA4C

UniProt

Q9C0C4 (Human)

Additional Semaphorin 4C Products

Product Documents for Human Semaphorin 4C Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Semaphorin 4C Antibody

For research use only

Related Research Areas

Proteoglycans

Citations for Human Semaphorin 4C Antibody

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