Detection of Human Semaphorin 4C by Western Blot.
Western blot shows lysates of MCF‑7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human Semaphorin 4C Monoclonal Antibody (Catalog # MAB6125) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Semaphorin 4C at approximately 100 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 4C
Semaphorin 4C (Sema4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature human Semaphorin 4C is a type I transmembrane glycoprotein that is 813 amino acids (aa) in length. It contains a 643 aa extracellular region (aa 21-663) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499-551), and an Ig-like C2-type domain (aa 556-644). The cytoplasmic region interacts with PZD-domain containing proteins. There are three potential splice variants. One demonstrates an alternative start site at Met324, a second shows a deletion of aa 173-211, and a third contains an 89 aa substitution for aa 1-36. Over aa 21-663, human Semaphorin 4C shares 85% aa identity with mouse Semaphorin 4C.
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