Human Serpin A1/ alpha 1-Antitrypsin Antibody
Human Serpin A1/ alpha 1-Antitrypsin Antibody Summary
Glu25-Lys418
Accession # P01009
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Serpin A1/ alpha 1‑Antitrypsin by Western Blot. Western blot shows human plasma and lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin A1/a1-Antitrypsin Monoclonal Antibody (Catalog # MAB1268) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Serpin A1/ alpha 1‑Antitrypsin in HepG2 Human Cell Line. Serpin A1/a1-Antitrypsin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Serpin A1/ a1-Antitrypsin Monoclonal Antibody (Catalog # MAB1268) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human Serpin A1/ alpha 1‑Antitrypsin by Simple WesternTM. Simple Western lane view shows lysates of human plasma, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 65 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Serpin A1/ alpha 1‑Antitrypsin Monoclonal Antibody (Catalog # MAB1268). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A1/alpha 1-Antitrypsin
Serpin A1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors (1). As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in the liver and secreted into the bloodstream with the major function to protect tissues against neutrophil elastase. A severe Serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma (2). The deficiency is caused by point mutations in naturally occurring Serpin A1 variants (over 70 are known). For example, the Z variant (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe Serpin A1 deficiency (3).
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Barbour, K.W. et al. (2002) Genomics 80:515.
- Lomas, D.A. et al. (2002) Biochem. Soc. Trans. 30:89.
Product Datasheets
Citations for Human Serpin A1/ alpha 1-Antitrypsin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Protease-anti-protease compartmentalization in SARS-CoV-2 ARDS: Therapeutic implications
Authors: OF McElvaney, T Asakura, SL Meinig, JL Torres-Cas, RS Hagan, C Gabillard, MP Murphy, LB Thorne, A Borczuk, EP Reeves, RE Zumwalt, Y Mikami, TP Carroll, K Okuda, G Hogan, OJ McElvaney, J Clarke, NL McEvoy, PW Mallon, C McCarthy, G Curley, MC Wolfgang, RC Boucher, NG McElvaney
EBioMedicine, 2022;77(0):103894.
Species: Human
Sample Types: Plasma
Applications: Immunoprecipitation -
Fecal host biomarkers predicting severity of Clostridioides difficile infection
Authors: M Golizeh, K Winter, L Roussel, M Landekic, M Langelier, VG Loo, M Ndao, DC Vinh
JCI Insight, 2020;0(0):.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Modified serpinA1 as risk marker for Parkinson's disease dementia: Analysis of baseline data
Sci Rep, 2016;6(0):26145.
Species: Human
Sample Types: CSF
Applications: Western Blot -
Maggot chymotrypsin I from Lucilia sericata is resistant to endogenous wound protease inhibitors.
Authors: Telford G, Brown AP, Kind A, English JS, Pritchard DI
Br. J. Dermatol., 2011;164(1):192-6.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
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