Human Serpin A1/ alpha 1-Antitrypsin Antibody

(2 citations)
(3 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Serpin A1/ alpha 1‑Antitrypsin in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant human Serpin A3, A4, A5, C1, F1, recombinant mouse Serpin D1, or F2 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 202808
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Serpin A1/ alpha 1‑Antitrypsin
    Glu25-Lys418
    Accession # P01009
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human Serpin A1/ alpha 1-Antitrypsin (Catalog # 1268-PI), see our available Western blot detection antibodies
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Serpin A1/ alpha 1‑Antitrypsin by Western Blot. Western blot shows human plasma and lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin A1/ alpha 1‑Antitrypsin Monoclonal Antibody (Catalog # MAB1268) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Serpin A1/ alpha 1‑Antitrypsin in HepG2 Human Cell Line.

Serpin A1/ alpha 1‑Antitrypsin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Serpin A1/
alpha 1‑Antitrypsin Monoclonal Antibody (Catalog # MAB1268) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Serpin A1/ alpha 1‑Antitrypsin by Simple WesternTM. Simple Western lane view shows lysates of human plasma, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 65 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Serpin A1/ alpha 1‑Antitrypsin Monoclonal Antibody (Catalog # MAB1268). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.        
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A1/alpha 1-Antitrypsin

Serpin A1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors (1). As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in the liver and secreted into the bloodstream with the major function to protect tissues against neutrophil elastase. A severe Serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma (2). The deficiency is caused by point mutations in naturally occurring Serpin A1 variants (over 70 are known). For example, the Z variant (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe Serpin A1 deficiency (3).

  • References:
    1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
    2. Barbour, K.W. et al. (2002) Genomics 80:515.
    3. Lomas, D.A. et al. (2002) Biochem. Soc. Trans. 30:89.
  • Entrez Gene IDs:
    5265 (Human)
  • Alternate Names:
    A1A; A1AT; AATMGC23330; alpha 1-Antitrypsin; alpha 1-Proteinase Inhibitor; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; alpha-1-antitrypsin; antitrypsin), member 1; member 1; PIMGC9222; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; serine (or cysteine) proteinase inhibitor, clade A, member 1; Serpin A1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Modified serpinA1 as risk marker for Parkinson's disease dementia: Analysis of baseline data
    Sci Rep, 2016;6(0):26145.
    Species: Human
    Sample Type: CSF
    Application: WB
  2. Maggot chymotrypsin I from Lucilia sericata is resistant to endogenous wound protease inhibitors.
    Authors: Telford G, Brown AP, Kind A, English JS, Pritchard DI
    Br. J. Dermatol., 2011;164(1):192-6.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 14
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ExactaChIP Chromatin Immunoprecipitation Kits
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ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
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Isotype Controls
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Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Mouse IgG2A Antibody

Flow MAB0032  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Average Rating: 4.7 (Based on 3 reviews)

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Read what people are saying who have used Human Serpin A1/ alpha 1-Antitrypsin Antibody.

We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 2 applications: ELISA, Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
  Very Good
 ELISA Human Anonymous 12/05/2017
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Summary

ApplicationELISA
Sample TestedSera
SpeciesHuman
Western Blot Human Serpin A1/ alpha 1-Antitrypsin Antibody MAB1268
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Excellent
 WB Human Anonymous 05/12/2017
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Western Blot Human Serpin A1/ alpha 1-Antitrypsin Antibody MAB1268
Western Blot: Human Serpin A1/ alpha 1-Antitrypsin Antibody [MAB1268]

Summary

ApplicationWestern Blot
Sample TestedPurified protein
SpeciesHuman
  Excellent
 ELISA Human Anonymous 04/26/2016
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Summary

ApplicationELISA
Sample TestedCell Lysates
SpeciesHuman

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