Human Serpin A1 DuoSet ELISA

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Human Serpin A1 / alpha 1-Antitrypsin ELISA Standard Curve
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Product Details
Citations (10)
Reviews (2)

Human Serpin A1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Serpin A1 / alpha 1-Antitrypsin. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Human Serpin A1 / alpha 1-Antitrypsin ELISA Standard Curve

Product Datasheets

Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin A1/alpha 1-Antitrypsin

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Entrez Gene IDs:
5265 (Human)
Alternate Names:
A1A; A1AT; AATMGC23330; alpha 1-Antitrypsin; alpha 1-Proteinase Inhibitor; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; alpha-1-antitrypsin; antitrypsin), member 1; member 1; PIMGC9222; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; serine (or cysteine) proteinase inhibitor, clade A, member 1; Serpin A1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Citations for Human Serpin A1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Alpha-1 antitrypsin deficiency impairs lung antibacterial immunity in mice
    Authors: L Ostermann, R Maus, J Stolper, L Schütte, K Katsarou, S Tumpara, A Pich, C Mueller, S Janciauski, T Welte, UA Maus
    JCI Insight, 2021;6(3):.
    Species: Mouse
    Sample Types: Plasma
  2. Reduced concentrations of the B cell cytokine Interleukin 38 are associated with cardiovascular disease risk in overweight subjects
    Authors: DM de Graaf, M Jaeger, ICL van den Mu, RT Horst, K Schraa, J Zwaag, M Kox, M Fujita, T Yamauchi, L Mercurio, S Madonna, JHW Rutten, J de Graaf, NP Riksen, FL van de Vee, MG Netea, LAB Joosten, CA Dinarello
    Eur J Immunol, 2020;0(0):.
    Species: Human
    Sample Types: Plasma
  3. Tri-antennary tri-sialylated mono-fucosylated glycan of alpha-1 antitrypsin as a non-invasive biomarker for non-alcoholic steatohepatitis: a novel glycobiomarker for non-alcoholic steatohepatitis
    Authors: K Ogawa, T Kobayashi, JI Furukawa, H Hanamatsu, A Nakamura, K Suzuki, N Kawagishi, M Ohara, M Umemura, M Nakai, T Sho, G Suda, K Morikawa, M Baba, K Furuya, K Terashita, T Kobayashi, M Onodera, T Horimoto, K Shinada, S Tsunematsu, I Tsunematsu, T Meguro, T Mitsuhashi, M Hato, K Higashino, Y Shinohara, N Sakamoto
    Sci Rep, 2020;10(1):321.
    Species: Human
    Sample Types: Serum
  4. Concerted actions by MMPs, ADAMTS and serine proteases during remodeling of the cartilage callus into bone during osseointegration of hip implants
    Authors: J Cassuto, A Folestad, J Göthlin, H Malchau, J Kärrholm
    Bone Rep, 2020;13(0):100715.
    Species: Human
    Sample Types: Plasma
  5. Fecal host biomarkers predicting severity of Clostridioides difficile infection
    Authors: M Golizeh, K Winter, L Roussel, M Landekic, M Langelier, VG Loo, M Ndao, DC Vinh
    JCI Insight, 2020;0(0):.
    Species: Mouse
    Sample Types: Feces
  6. Circulating Truncated Alpha-1 Antitrypsin Glycoprotein in Patient Plasma Retains Anti-Inflammatory Capacity
    Authors: EP Reeves, DM Dunlea, K McQuillan, CA O'Dwyer, TP Carroll, R Saldova, PR Akepati, MR Wormald, OJ McElvaney, V Shutchaida, M Henry, P Meleady, J Keenan, DC Liberti, DN Kotton, PM Rudd, AA Wilson, NG McElvaney
    J. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Differential bronchial epithelial response regulated by ?Np63: a functional understanding of the epithelial shedding found in asthma
    Authors: T Kubo, M Tsujiwaki, Y Hirohashi, T Tsukahara, T Kanaseki, M Nakatsugaw, T Hasegawa, T Torigoe
    Lab. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Linking the Human Gut Microbiome to Inflammatory Cytokine Production Capacity
    Authors: Ramnik J Xavier
    Cell, 2016;167(4):1125-1136.e8.
    Species: Human
    Sample Types: Plasma
  9. Host and Environmental Factors Influencing Individual Human Cytokine Responses
    Authors: Mihai G Netea
    Cell, 2016;167(4):1111-1124.e13.
    Species: Human
    Sample Types: Plasma
  10. Rapid renal alpha-1 antitrypsin gene induction in experimental and clinical acute kidney injury.
    Authors: Zager R, Johnson A, Frostad K
    PLoS ONE, 2014;9(5):e98380.
    Species: Human
    Sample Types: Urine


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Reviews for Human Serpin A1 DuoSet ELISA

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Human Serpin A1 DuoSet ELISA
By Anonymous on 10/20/2021
Application: Sample Tested: 293T human embryonic kidney cell line

I used this kit to detect the human AAT expressed in mice and it worked like a charm. No background with the mice AAT as reported. The kit is very sensitive and the procedure is straightforward.

Human Serpin A1 DuoSet ELISA
By Anonymous on 09/28/2020
Application: Sample Tested: Serum

Kit is very sensitive and samples had to be diluted a lot in a serial dilution.
The kit is specific for human A1AT and no cross-reactivity with mouse A1AT was observed. The kit is very reproducible.
We used Thermo Fisher's QuantaBlu substrate for detection.