Human Serpin A1 DuoSet ELISA

R&D Systems | Catalog # DY1268

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

125-8000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human Serpin A1 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all Serpin A1/alpha 1-Antitrypsin ELISA Kits »

Product Summary for Human Serpin A1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Serpin A1 / alpha 1-Antitrypsin. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human Serpin A1 DuoSet ELISA

Human Serpin A1 / alpha 1-Antitrypsin ELISA Standard Curve

Human Serpin A1 / alpha 1-Antitrypsin ELISA Standard Curve

Kit Contents for Human Serpin A1 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS:

(Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin A1/alpha 1-Antitrypsin

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Alternate Names

A1AT, alpha 1-Antitrypsin, alpha 1-Proteinase Inhibitor

Entrez Gene IDs

5265 (Human)

Gene Symbol

SERPINA1

Additional Serpin A1/alpha 1-Antitrypsin Products

Product Documents for Human Serpin A1 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Serpin A1 DuoSet ELISA

For research use only

Citations for Human Serpin A1 DuoSet ELISA

Customer Reviews for Human Serpin A1 DuoSet ELISA (3)

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  • Human Serpin A1 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Whole blood
    Verified Customer | Posted 11/16/2025
  • Human Serpin A1 DuoSet ELISA
    Name: Anonymous
    Sample Tested: 293T human embryonic kidney cell line
    Verified Customer | Posted 10/20/2021
    I used this kit to detect the human AAT expressed in mice and it worked like a charm. No background with the mice AAT as reported. The kit is very sensitive and the procedure is straightforward.
  • Human Serpin A1 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 09/28/2020
    Kit is very sensitive and samples had to be diluted a lot in a serial dilution. The kit is specific for human A1AT and no cross-reactivity with mouse A1AT was observed. The kit is very reproducible. We used Thermo Fisher's QuantaBlu substrate for detection.
    Human Serpin A1 DuoSet ELISA DY1268

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