Detection of Human Serpin A6 by Western Blot. Western blot shows lysates of human plasma and human serum. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Serpin A6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9026) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Serpin A6 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A6
Serpin A6, also known as Corticosteroid-binding Globulin/CBG and Transcortin, is a 45‑60 kDa secreted glycoprotein member of the clade A serpin family of protease inhibitors. Although it is a serpin family member, it shows no protease inhibition. Serpin A6 is produced principally by hepatocytes, but is also expressed by hypothalamic neurons and anterior pituitary endocrine cells. Serpin A6 transports progesterone and > 90% of circulating cortisol, and likely has three functions: first it can be cleaved, releasing cortisol to the tissues; second, it regulates levels of free hormone available over time; and third, it likely binds to specific cell-surface receptors when complexed to cortisol. Human Serpin A6 is 405 amino acids (aa) in length. Over aa 23‑396, human Serpin A6 shares 57% and 60% aa sequence identity with mouse and rat Serpin A6, respectively.
Entrez Gene IDs:
866 (Human); 12401 (Mouse); 299270 (Rat)
antitrypsin), member 6; CBG; CBGcorticosteroid-binding globulin; corticosteroid binding globulin; member 6; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A6; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); transcortin
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