Serpin B5, also known as Maspin, is a secreted member of the Serpin family although it has no known serine protease inhibitor activity. It is produced by mammary epithelial cells and functions as a tumor suppressor. Serpin B5 is a valuable biomarker of tumor progression and overall prognosis. The amino acid sequence of human Serpin B5 is 99%, 92%, 89%, 88% and 64% identical to that of chimpanzee, dog, mouse, rat and chicken.
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Knockout Validated, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 305339
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Product Specifications
Immunogen
E. coli-derived recombinant human Serpin B5
Met1-Pro375
Accession # P36952
Met1-Pro375
Accession # P36952
Specificity
Detects human Serpin B5/Maspin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Serpin A5, B6, B8 or B9 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human Serpin B5/Maspin Antibody
Detection of Human Serpin B5/Maspin by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-3 human prostate cancer cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Serpin B5/Maspin Monoclonal Antibody (Catalog # MAB2218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Serpin B5/Maspin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human Serpin B5/Maspin Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Serpin B5/Maspin knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Serpin B5/Maspin Monoclonal Antibody (Catalog # MAB2218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Serpin B5/Maspin at approximately 40 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Serpin B5/Maspin Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin B5/Maspin, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin B5/Maspin, see our available Western blot detection antibodies
Knockout Validated
Serpin B5/Maspin
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
Serpin B5/Maspin knockout HeLa cell line.
Western Blot
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, PC‑3 human prostate cancer cell line, and RT-4 human bladder carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, PC‑3 human prostate cancer cell line, and RT-4 human bladder carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Serpin B5/Maspin
Additional Serpin B5/Maspin Products
Product Documents for Human Serpin B5/Maspin Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Serpin B5/Maspin Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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