Human Serpin F1/PEDF DuoSet ELISA

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Human Serpin F1 / PEDF ELISA Standard Curve
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Product Details
Citations (16)
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Human Serpin F1/PEDF DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
78.1 - 5,000 pg/mL
Sufficient Materials
For five 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Serpin F1 / PEDF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human Serpin F1 / PEDF ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin F1/PEDF

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Long Name:
Serpine F1/Pigment Epithelium-derived Factor
Entrez Gene IDs:
5176 (Human); 20317 (Mouse); 287526 (Rat)
Alternate Names:
Cell proliferation-inducing gene 35 protein; EPC-1; EPC-1PIG35; PEDF; PEDFpigment epithelium-derived factor; pigment epithelium derived factor), member 1; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1

Citations for Human Serpin F1/PEDF DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Oxidative stress differentially impacts apical and basolateral secretion of angiogenic factors from human iPSC-derived retinal pigment epithelium cells
    Authors: L Chen, ND Perera, AJ Karoukis, KL Feathers, RR Ali, DA Thompson, AT Fahim
    Scientific Reports, 2022;12(1):12694.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Inflammasome Activation in Retinal Pigment Epithelium from Human Donors with Age-Related Macular Degeneration
    Authors: MC Ebeling, CR Fisher, RJ Kapphahn, MR Stahl, S Shen, J Qu, SR Montezuma, DA Ferrington
    Cells, 2022;11(13):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Impaired Mitochondrial Function in iPSC-Retinal Pigment Epithelium with the Complement Factor H Polymorphism for Age-Related Macular Degeneration
    Authors: MC Ebeling, Z Geng, RJ Kapphahn, H Roehrich, SR Montezuma, JR Dutton, DA Ferrington
    Cells, 2021;10(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Identification of soluble tissue-derived biomarkers from human thyroid tissue explants maintained on a microfluidic device
    Authors: A Riley, H Jones, J England, D Kuvshinov, V Green, J Greenman
    Oncology Letters, 2021;22(5):780.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Conditioned Medium from Human Mesenchymal Stromal Cells: Towards the Clinical Translation
    Authors: G Sagaradze, O Grigorieva, P Nimiritsky, N Basalova, N Kalinina, Z Akopyan, A Efimenko
    Int J Mol Sci, 2019;20(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. The contrary intracellular and extracellular functions of PEDF in HCC development
    Authors: C Li, Z Huang, L Zhu, X Yu, T Gao, J Feng, H Hong, H Yin, T Zhou, W Qi, Z Yang, C Liu, X Yang, G Gao
    Cell Death Dis, 2019;10(10):742.
    Species: Xenograft
    Sample Types: Serum
  7. Cancer-Targeted PEDF-DNA Therapy for Metastatic Colorectal Cancer
    Authors: X Bao, J Zeng, H Huang, C Ma, L Wang, F Wang, X Liao, X Song
    Int J Pharm, 2019;0(0):118999.
    Species: Mouse
    Sample Types: Serum
  8. Pigment Epithelial-Derived Factor Deficiency Accelerates Atherosclerosis Development via Promoting Endothelial Fatty Acid Uptake in Mice With Hyperlipidemia
    Authors: H Wang, Y Yang, M Yang, X Li, J Tan, Y Wu, Y Zhang, Y Li, B Hu, S Deng, F Yang, S Gao, H Li, Z Yang, H Chen, W Cai
    J Am Heart Assoc, 2019;8(22):e013028.
    Species: Human
    Sample Types: Serum
  9. Zika virus induces strong inflammatory responses and impairs homeostasis and function of the human retinal pigment epithelium
    Authors: Y Simonin, N Erkilic, K Damodar, M Clé, C Desmetz, K Bolloré, M Taleb, S Torriano, J Barthelemy, G Dubois, AD Lajoix, V Foulongne, E Tuaillon, P Van de Per, V Kalatzis, S Salinas
    EBioMedicine, 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Mutant Best1 Expression and Impaired Phagocytosis in an iPSC Model of Autosomal Recessive Bestrophinopathy
    Authors: AD Marmorstei, AA Johnson, LA Bachman, C Andrews-Pf, T Knudsen, BJ Gilles, M Hill, JK Gandhi, LY Marmorstei, JS Pulido
    Sci Rep, 2018;8(1):4487.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Novel mutations of the SERPINF1 and FKBP10 genes in Chinese families with autosomal recessive osteogenesis imperfecta
    Authors: H Zhang, Y Xu, H Yue, C Wang, J Gu, J He, W Fu, W Hu, Z Zhang
    Int. J. Mol. Med., 2018;0(0):.
    Species: Human
    Sample Types: Serum
  12. Corneal Mesenchymal Stromal Cells Are Directly Antiangiogenic via PEDF and sFLT-1
    Authors: M Eslani, I Putra, X Shen, J Hamouie, N Afsharkham, S Besharat, MI Rosenblatt, R Dana, P Hematti, AR Djalilian
    Invest. Ophthalmol. Vis. Sci., 2017;58(12):5507-5517.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Effects of Ranibizumab and Aflibercept on Human M�ller Cells and Photoreceptors under Stress Conditions
    Authors: W Shen, B Yau, SR Lee, L Zhu, M Yam, MC Gillies
    Int J Mol Sci, 2017;18(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Altered bioenergetics and enhanced resistance to oxidative stress in human retinal pigment epithelial cells from donors with age-related macular degeneration
    Authors: DA Ferrington, MC Ebeling, RJ Kapphahn, MR Terluk, CR Fisher, JR Polanco, H Roehrich, MM Leary, Z Geng, JR Dutton, SR Montezuma
    Redox Biol, 2017;13(0):255-265.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Promising Nanocarriers for PEDF Gene Targeting Delivery to Cervical Cancer Cells Mediated by the Over-expressing FR?
    Sci Rep, 2016;6(0):32427.
    Species: Mouse
    Sample Types: Serum
  16. Porous poly(epsilon-caprolactone) scaffolds for retinal pigment epithelium transplantation.
    Authors: McHugh K, Tao S, Saint-Geniez M
    Invest Ophthalmol Vis Sci, 2014;55(3):1754-62.
    Species: Human
    Sample Types: Cell Culture Supernates


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Human Serpin F1/PEDF DuoSet ELISA
By Andrew Riley on 09/02/2019
Sample Tested: Tissue Culture Media

Assayed culture effluent (supernatant) from ex vivo human thyroid tissue