Human Serpin F1/PEDF DuoSet ELISA

Catalog # Availability Size / Price Qty
DY1177-05
Ancillary Products Available
Human Serpin F1 / PEDF ELISA Standard Curve
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Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (1)

Human Serpin F1/PEDF DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Serpin F1 / PEDF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Human Serpin F1 / PEDF ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin F1/PEDF

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Long Name:
Serpine F1/Pigment Epithelium-derived Factor
Entrez Gene IDs:
5176 (Human); 20317 (Mouse); 287526 (Rat)
Alternate Names:
Cell proliferation-inducing gene 35 protein; EPC-1; EPC-1PIG35; PEDF; PEDFpigment epithelium-derived factor; pigment epithelium derived factor), member 1; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1

Citations for Human Serpin F1/PEDF DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Conditioned Medium from Human Mesenchymal Stromal Cells: Towards the Clinical Translation
    Authors: G Sagaradze, O Grigorieva, P Nimiritsky, N Basalova, N Kalinina, Z Akopyan, A Efimenko
    Int J Mol Sci, 2019;20(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Zika virus induces strong inflammatory responses and impairs homeostasis and function of the human retinal pigment epithelium
    Authors: Y Simonin, N Erkilic, K Damodar, M Clé, C Desmetz, K Bolloré, M Taleb, S Torriano, J Barthelemy, G Dubois, AD Lajoix, V Foulongne, E Tuaillon, P Van de Per, V Kalatzis, S Salinas
    EBioMedicine, 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Mutant Best1 Expression and Impaired Phagocytosis in an iPSC Model of Autosomal Recessive Bestrophinopathy
    Authors: AD Marmorstei, AA Johnson, LA Bachman, C Andrews-Pf, T Knudsen, BJ Gilles, M Hill, JK Gandhi, LY Marmorstei, JS Pulido
    Sci Rep, 2018;8(1):4487.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Novel mutations of the SERPINF1 and FKBP10 genes in Chinese families with autosomal recessive osteogenesis imperfecta
    Authors: H Zhang, Y Xu, H Yue, C Wang, J Gu, J He, W Fu, W Hu, Z Zhang
    Int. J. Mol. Med., 2018;0(0):.
    Species: Human
    Sample Types: Serum
  5. Corneal Mesenchymal Stromal Cells Are Directly Antiangiogenic via PEDF and sFLT-1
    Authors: M Eslani, I Putra, X Shen, J Hamouie, N Afsharkham, S Besharat, MI Rosenblatt, R Dana, P Hematti, AR Djalilian
    Invest. Ophthalmol. Vis. Sci., 2017;58(12):5507-5517.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Effects of Ranibizumab and Aflibercept on Human M�ller Cells and Photoreceptors under Stress Conditions
    Authors: W Shen, B Yau, SR Lee, L Zhu, M Yam, MC Gillies
    Int J Mol Sci, 2017;18(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Altered bioenergetics and enhanced resistance to oxidative stress in human retinal pigment epithelial cells from donors with age-related macular degeneration
    Authors: DA Ferrington, MC Ebeling, RJ Kapphahn, MR Terluk, CR Fisher, JR Polanco, H Roehrich, MM Leary, Z Geng, JR Dutton, SR Montezuma
    Redox Biol, 2017;13(0):255-265.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Promising Nanocarriers for PEDF Gene Targeting Delivery to Cervical Cancer Cells Mediated by the Over-expressing FR?
    Sci Rep, 2016;6(0):32427.
    Species: Mouse
    Sample Types: Serum
  9. Porous poly(epsilon-caprolactone) scaffolds for retinal pigment epithelium transplantation.
    Authors: McHugh K, Tao S, Saint-Geniez M
    Invest Ophthalmol Vis Sci, 2014;55(3):1754-62.
    Species: Human
    Sample Types: Cell Culture Supernates

FAQs

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Reviews for Human Serpin F1/PEDF DuoSet ELISA

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Human Serpin F1/PEDF DuoSet ELISA
By Anonymous on 09/02/2019
Sample Tested: Tissue Culture Media

Assayed culture effluent (supernatant) from ex vivo human thyroid tissue