Human Serum TIM-1/KIM-1/HAVCR Quantikine ELISA Kit

  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    3.63 pg/mL
  • Assay Range
    10.9 - 700 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human TIM-1.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human Serum TIM-1/KIM-1/HAVCR Immunoassay is a 4.5 hour solid phase ELISA designed to measure human TIM-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human TIM-1 and antibodies raised against the recombinant factor. Natural human TIM-1 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative levels of natural human TIM-1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean48.914829551.1149292
Standard Deviation1.653.928.73.378.9719.6
CV%3.42.62.96.666.7

Recovery

The recovery of human TIM-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 94 89-102
EDTA Plasma (n=4) 93 80-108
Heparin Plasma (n=4) 91 81-104
Serum (n=4) 94 82-109
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human TIM-1 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Serum TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TIM-1/KIM-1/HAVCR
TIM-1 (T cell immunoglobulin and mucin domain 1), also known as KIM-1 and HAVcr1, is expressed on many immune cell types and epithelial cells. It binds to phosphatidylserine (PS), LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses. Its interaction with PS enables TIM-1 to mediate the phagocytosis and clearance of apoptotic cells. TIM-1 signaling costimulates T cell activation and enhances Th2 cytokine production. TIM-1 serves as a cellular entry receptor for hepatitis A virus, Ebolavirus and Marburgvirus. Polymorphisms are associated with susceptibility to atopy, autoimmunity, and severe hepatitis A virus infection in humans. A soluble form of TIM-1 is elevated in the urine during nephropathy.
    • Long Name
      T Cell Immunoglobulin Mucin-1
    • Entrez Gene IDs
      26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey);
    • Alternate Names
      CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1;
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