Human Siglec-1/CD169 APC-conjugated Antibody

Recombinant Monoclonal Antibody.
  • Species Reactivity
  • Specificity
    Detects human Siglec‑1/CD169 in direct ELISAs.
  • Source
    Recombinant Monoclonal Mouse IgG1 Clone # 908102
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Siglec‑1/CD169
    Accession # Q9BZZ2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Siglec‑1/CD169 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs), (A) resting, or (B) treated with 20 ng/mL Recombinant Human IFN‑ gamma (Catalog # 285-IF) for 24 hours, were stained with Mouse Anti-Human Siglec‑1/CD169 APC‑conjugated Monoclonal Antibody (Catalog # FAB5197A) and Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Siglec-1/CD169

Siglecs are sialic acid specific I-type lectins that belong to the immunoglobulin superfamily. Structurally, they are transmembrane proteins with an N-terminal Ig-like V‑set domain followed by varying numbers of Ig-like C2-set domains (1, 2). Human Siglec-1, also known as sialoadhesin and CD169, is a 175‑185 kDa glycoprotein. It contains a 1622 amino acid (aa) extracellular domain (ECD) with one Ig-like V‑set domain and 16 Ig-like C2-set domains, a 21 aa transmembrane segment, and a 47 aa cytoplasmic domain (3). Within the ECD, human Siglec-1 shares approximately 70% aa sequence identity with mouse and rat Siglec-1. Alternate splicing generates a potentially soluble form of the ECD, while a second isoform shows a substituted cytoplasmic domain. Siglec-1 expression occurs on monocytes, dendritic cells, and macrophages of various organs and tissues, including liver, spleen, lung, and gut. The adhesive function of Siglec-1 is supported by the N-terminal Ig-like domain which shows a selectivity for alpha 2,3‑linked sialic acid residues (3‑5). Siglec-1 binds a number of sialylated molecules including the mannose receptor, MGL1, MUC1, PSGL-1, and different glycoforms of CD43 (6‑9). Its binding capacity can be masked by endogenous sialylated molecules (10, 11). The sialylated and sulfated N-linked carbohydrates that modify Siglec-1 itself are required for ligand binding (6, 7). Siglec-1 is expressed on dendritic cells following rhinovirus exposure, and these DC promote T cell anergy (12). It is also induced on circulating monocytes during systemic sclerosis and HIV-1 infection (13‑15). Siglec-1 can trap HIV-1 particles for trans infection of permissive cells (14).

  • References:
    1. Varki, A. and T. Angata (2006) Glycobiology 16:1R.
    2. Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
    3. Hartnell, A. et al. (2001) Blood 97:288.
    4. Nath, D. et al. (1995) J. Biol. Chem. 270:26184.
    5. Crocker, P.R. et al. (1991) EMBO J. 10:1661.
    6. Martinez-Pomares, L. et al. (1999) J. Biol. Chem. 274:35211.
    7. Kumamoto, Y. et al. (2004) J. Biol. Chem. 279:49274.
    8. Nath, D. et al. (1999) Immunology 98:213.
    9. van den Berg, T.K. et al. (2001) J. Immunol. 166:3637.
    10. Nakamura, K. et al. (2002) Glycobiology 12:209.
    11. Barnes, Y.C. et al. (1999) Blood 93:1245.
    12. Kirchberger, S. et al. (2005) J. Immunol. 175:1145.
    13. York, M.R. et al. (2007) Arthritis Rheum. 56:1010.
    14. Rempel, H. et al. (2008) PloS ONE 3:e1967.
    15. van der Kuyl, A.C. et al. (2007) Plos ONE 2:e257.
  • Long Name:
    Sialic Acid Binding Ig-like Lectin 1
  • Entrez Gene IDs:
    6614 (Human); 20612 (Mouse); 311426 (Rat)
  • Alternate Names:
    C19orf75; CD169; Siglec1; Siglec-1; SIGLECL1 SIGLEC family like 1
Related Research Areas
Isotype Controls
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Mouse IgG1 APC-conjugated Antibody

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