Human SIRP alpha /CD172a
Accession # P78324
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human SIRP alpha /CD172a
by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, A549 human lung carcinoma cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SIRP alpha /CD172a
Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4546) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for SIRP alpha /CD172a
at approximately 70-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Human SIRP alpha /CD172a by Simple WesternTM. Simple Western lane view shows lysates of U2OS human osteosarcoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SIRPa/CD172a at approximately 113-116 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human SIRPa/CD172a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4546) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SIRP alpha/CD172a
Signal regulatory protein alpha (SIRP alpha, designated CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), is a monomeric ~90 kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily (1‑4). SIRPs are paired receptors, with similar extracellular domains but differing C-termini and functions (1, 2). The 503 amino acid (aa) human SIRP alpha contains a 342 aa extracellular domain (ECD), with one V-type, and two C1 type Ig domains, and three potential N glycosylation sites. It has a 110 aa cytoplasmic sequence with ITIM motifs that recruit tyrosine phosphatases SHP-1 and SHP-2 when phosphorylated (4). Human SIRP alpha has more than 40 described polymorphisms, including the prominent BIT (Brain Ig like molecule with Tyrosine-based activation motifs, also called SIRP alpha 2 or PTPNS) (5). One reported isoform lacks aa 1‑101, which eliminates most of the V type Ig domain. Human SIRP alpha ECD shares 61%, 60%, 71%, 72% and 73% aa identity with mouse, rat, porcine, bovine and equine SIRP alpha, respectively; it shares 84% and 76% aa identity with human SIRP beta 1 and SIRP gamma, respectively (2). SIRP alpha is expressed mainly on myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells (3‑6). It is also found on neurons, smooth muscle and endothelial cells (7‑9). SIRP alpha shows adhesion to the ubiquitous CD47/IAP (integrin associated protein), while SIRP gamma binds more weakly and SIRP alpha 1 does not bind at all (1, 2). Mouse and human SIRP alpha -CD47 binding only cross-reacts for specific polymorphisms and influences engraftment of xenotransplanted stem cells (6, 10). SIRP alpha engagement generally produces a negative regulatory signal (4). Low SIRP alpha recognition of CD47, which occurs on aged erythrocytes or platelets or xenogenic cells, promotes clearance of CD47low cells from circulation (11, 13). SIRP alpha recognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production (14). The CD47 integrin-SIRP alpha interaction is reported to promote macrophage fusion during osteoclastogenesis (15).
- Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457.
- vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
- Liu, Y. et al. (2005) J. Biol. Chem. 280:36132.
- Kharitonenkov, A. et al. (1997) Nature 386:181.
- Swissprot Accession # P78324.
- Miyashita, M. et al. (2004) Mol. Biol. Cell 15:3950.
- Wang, X.X. & K.H. Pfenninger (2005) J. Cell Sci. 119:172.
- Maile, L.A. et al. (2003) Mol. Biol. Cell 14:3519.
- Johansen, M.L. & E.J. Brown (2007) J. Biol. Chem. 282:24219.
- Takenaka, K. et al. (2007) Nat. Immunol. 8:1313.
- Ishikawa-Sekigami, T. et al. (2006) Biochem. Biophys. Res. Commun. 343:1197.
- Olsson, M. et al. (2005) Blood 105:3577.
- Ide, K. et al. (2007) Proc. Natl. Acad. Sci. USA 104:5062.
- Gardai, S.J. et al. (2003) Cell 115:13.
- Lundberg, P. et al. (2007) Biochem. Biophys. Res. Commun. 352:444.
Citation for Human SIRP alpha /CD172a
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
A New Serum Macrophage Checkpoint Biomarker for Innate Immunotherapy: Soluble Signal-Regulatory Protein Alpha (sSIRPalpha)
Authors: YV Vladimirov, MK Mølmer, KW Antonsen, N Møller, N Rittig, MC Nielsen, HJ Møller
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
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