Detection of Human Skp2 by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human Skp2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6817) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Skp2 at approximately 45 and 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Skp2 (S-phase kinase-associated protein 2; also F-box/LRR protein 1) is a 45-49 kDa member of the F-box protein family. It is known to be part of the SCF (Skp1/Cul1/F-box protein) complex. The SCF complex functions as an E3 ubiquitin-protein ligase that mediates the transfer of ubiquitin from an E2 enzyme to a target substrate. The specificity of the target is determined by Skp2. Molecules targeted for degradation include cell-cycle regulatory proteins that are involved in the G1-S phase transition. Human Skp2 is 424 amino acids (aa) in length. It contains one F-box (aa 94-140) followed by nine consecutive Leu-rich repeats (aa 142-369). There are two potential splice forms that contain substitutions for the same aa sequence. One contains a 4 aa substitution, while a second contains a 56 aa substitution for aa 354-424. Over aa 130-350, human Skp2 shares 84% aa identity with mouse Skp2.
S Phase Kinase-associated Protein 2
Entrez Gene IDs:
6502 (Human); 27401 (Mouse); 294790 (Rat)
CDK2/cyclin A-associated protein p45; cyclin A/CDK2-associated protein p45; Cyclin-A/CDK2-associated protein p45; FBL1; F-box protein Skp2; F-box/LRR-repeat protein 1; FBXL1; FBXL1MGC1366; FLB1; p45skp2; Skp2; S-phase kinase-associated protein 2 (p45); S-phase kinase-associated protein 2
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