Human sL-Selectin/CD62L ELISA Kit

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  (5 citations)
(1 Review)
    
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (5)
FAQs
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    2 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
  • Sensitivity
    0.3 ng/mL
  • Assay Range
    1.0 - 58 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human sL-Selectin
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Human sL-Selectin Immunoassay is a 2 hour solid phase ELISA that is designed to measure sL-Selectin in cell culture supernates, serum, and plasma. It contains recombinant human sL-Selectin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sL-Selectin showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human sL-Selectin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 21 21 21
Mean 210 881 3025 209 882 3083
Standard Deviation 0 0 0 0 0 0
CV% 5 2.5 4.1 11.9 8.8 7.1

Recovery

The recovery of sL-Selectin, spiked to three different levels ranging from 475-3784 ng/mL in various matrices, was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 100 92-106
EDTA Plasma (n=5) 102 93-109
Serum (n=5) 103 88-114
Linearity
To assess linearity of the assay two cell culture supernates and five spiked serum and plasma samples were diluted in Sample Diluent and assayed in three batches of kits.
 L-Selectin/CD62L [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

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Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: L-Selectin/CD62L
The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.
  • Entrez Gene IDs:
    6402 (Human); 20343 (Mouse); 29259 (Rat)
  • Alternate Names:
    CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, samples, and Control as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Standard, Control, or sample
  4.   Add 100 µL of Standard, Control, or sample to each well. Ensure sample addition is uninterrupted and completed within 15 minutes. Cover the plate with a plate sealer, and incubate at room temperature for 1 hour.

  5. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Tap the plate gently to mix. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
  7.   Aspirate or decant each well and wash, repeating the process 5 times for a total of 6 washes.

  8. 100 µL Substrate
  9.   Add 100 µL of Substrate to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.

  10. 100 µL Stop Solution
  11.   Add 100 µL Stop Solution to each well. The Stop Solution should be added to the wells in the same order as the Substrate. Read at 450 nm within 30 minutes. Set wavelength correction to 620 nm or 650 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Sample Type
  1. Inhibition of myeloperoxidase decreases vascular oxidative stress and increases vasodilatation in sickle cell disease mice.
    Authors: Zhang H, Xu H, Weihrauch D, Jones D, Jing X, Shi Y, Gourlay D, Oldham K, Hillery C, Pritchard K
    J Lipid Res, 2013;54(11):3009-15.
    Species: Human
    Sample Type: Plasma
  2. Prehospital resuscitation with hypertonic saline-dextran modulates inflammatory, coagulation and endothelial activation marker profiles in severe traumatic brain injured patients.
    Authors: Rhind SG, Crnko NT, Baker AJ
    J Neuroinflammation, 2010;7(0):5.
    Species: Human
    Sample Type: Serum
  3. Expression and regulation of the metalloproteinase ADAM-8 during human neutrophil pathophysiological activation and its catalytic activity on L-selectin shedding.
    Authors: Gomez-Gaviro M, Dominguez-Luis M, Canchado J, Calafat J, Janssen H, Lara-Pezzi E, Fourie A, Tugores A, Valenzuela-Fernandez A, Mollinedo F, Sanchez-Madrid F, Diaz-Gonzalez F
    J. Immunol., 2007;178(12):8053-63.
    Species: Human
    Sample Type: Cell Culture Supernates
  4. Regulation of peritoneal and systemic neutrophil-derived tumor necrosis factor-alpha release in patients with severe peritonitis: role of tumor necrosis factor-alpha converting enzyme cleavage.
    Authors: Kermarrec N, Selloum S, Plantefeve G, Chosidow D, Paoletti X, Lopez A, Mantz J, Desmonts JM, Gougerot-Pocidalo MA, Chollet-Martin S
    Crit. Care Med., 2005;33(6):1359-64.
    Species: Human
    Sample Type: Cell Culture Supernates
  5. Soluble adhesion molecules in juvenile rheumatoid arthritis.
    Authors: Bloom BJ, Miller LC, Tucker LB, Schaller JG, Blier PR
    J. Rheumatol., 1999;26(9):2044-8.
    Species: Human
    Sample Type: Serum

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Average Rating: 5 (Based on 1 review)

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