Human SLAM/CD150 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1642P
Detection of SLAM/CD150 in Human Lymphocytes by Flow Cytometry.
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Product Details
Citations (1)
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Human SLAM/CD150 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human SLAM/CD150 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 542301
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SLAM/CD150
Ala21-Pro237
Accession # Q13291
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of SLAM/CD150 in Human Lymphocytes by Flow Cytometry. View Larger

Detection of SLAM/CD150 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human SLAM/CD150 PE-conjugated Monoclonal Antibody (Catalog # FAB1642P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: SLAM/CD150

Signaling Lymphocytic Activation Molecule (SLAM), also known as SLAMF1 and CD150, was the first identified family member of type I transmembrane (TM) lymphocyte activating receptors. SLAM homotypic adhesion bidirectionally stimulates T and B cells. SLAM is also expressed by hematopoietic stem cells, dendritic cells and platelets and is a T cell measles virus receptor. The 70 kDa glycoprotein contains a 216 amino acid (aa) extracellular domain (ECD) with one C2 type and one V type Ig-like domain, a 20 aa TM sequence and a 76 aa SH2-binding cytoplasmic domain. One splice variant has a shorter cytoplasmic tail and another lacks the TM sequence and is secreted. Human and mouse SLAM ECD share 60% aa identity.

Long Name
Signaling Lymphocytic Activation Molecule
Entrez Gene IDs
6504 (Human); 27218 (Mouse); 102135470 (Cynomolgus Monkey)
Alternate Names
CD 150; CD150; CD150IPO-3; CDw150; IPO-3; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule; SLAM; SLAMCD150 antigen; SLAMF1

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Citation for Human SLAM/CD150 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Mev-stealth: A cd46-specific oncolytic measles virus resistant to neutralization by measles-immune human serum
    Authors: MÁ Muñoz-Alía, RA Nace, A Tischer, L Zhang, ES Bah, M Auton, SJ Russell
    PloS Pathogens, 2021;17(2):e1009283.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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