Human SLAM/CD150 Antibody

Catalog # Availability Size / Price Qty
MAB1642
MAB1642-SP
Detection of Human SLAM/CD150 by Western Blot.
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Product Details
Citations (2)
FAQs
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Human SLAM/CD150 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SLAM/CD150 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 542301
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SLAM/CD150
Ala21-Pro237
Accession # Q13291
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB16421).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human SLAM/CD150 DuoSet ELISA Kit (Catalog # DY164) for convenient development of a sandwich ELISA.

 
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human SLAM/CD150 by Western Blot. View Larger

Detection of Human SLAM/CD150 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with 10 ng/mL PMA and 200 ng/mL ionomycin for 48 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SLAM/CD150 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of SLAM/CD150 in Human Lymphocytes by Flow Cytometry. View Larger

Detection of SLAM/CD150 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Human SLAM/CD150 ELISA Standard Curve. Recombinant Human SLAM/CD150 protein was serially diluted 2-fold and captured by Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB16421) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLAM/CD150

Signaling lymphocytic activation molecule (SLAM, SLAMF1; CD150) was the first identified of a family of type I transmembrane (TM) lymphocyte activating receptors. SLAM homotypic adhesion bidirectionally stimulates T and B cells. SLAM is also expressed by hematopoietic stem cells, dendritic cells and platelets and is a T cell measles virus receptor. The 70 kDa glycoprotein contains a 216 amino acid (aa) extracellular domain (ECD) with one C2 type and one V type Ig-like domain, a 20 aa TM sequence and a 76 aa SH2-binding cytoplasmic domain. One splice variant has a shorter cytoplasmic tail and another lacks the TM sequence and is secreted. Human and mouse SLAM ECD share 60% aa identity.

Long Name
Signaling Lymphocytic Activation Molecule
Entrez Gene IDs
6504 (Human); 27218 (Mouse); 102135470 (Cynomolgus Monkey)
Alternate Names
CD 150; CD150; CD150IPO-3; CDw150; IPO-3; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule; SLAM; SLAMCD150 antigen; SLAMF1

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Citations for Human SLAM/CD150 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. SLAMF receptors negatively regulate B cell receptor signaling in chronic lymphocytic leukemia via recruitment of prohibitin-2
    Authors: L von Wenser, C Schulthei beta, S Bolz, S Schliffke, D Simnica, E Willscher, H Gerull, G Wolters-Ei, K Riecken, B Fehse, M Altfeld, P Nollau, M Binder
    Leukemia, 2020;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. SLAMF receptors negatively regulate B cell receptor signaling in chronic lymphocytic leukemia via recruitment of prohibitin-2
    Authors: L von Wenser, C Schulthei beta, S Bolz, S Schliffke, D Simnica, E Willscher, H Gerull, G Wolters-Ei, K Riecken, B Fehse, M Altfeld, P Nollau, M Binder
    Leukemia, 2020;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Co-Immunoprecipitation

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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