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Human SLC5A8/SMCT1 APC-conjugated Antibody

Catalog #: FAB8398A Datasheet / COA / SDS

Discontinued Product

FAB8398A has been discontinued.
View all SLC5A8/SMCT1 products.
Detection of SLC5A8/SMCT1 in MCF‑7 Human Cell Line by Flow Cytometry.
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Human SLC5A8/SMCT1 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects HEK293 human embryonic kidney cell line transfected with human SLC5A8/SMCT1 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
Source
Monoclonal Mouse IgG1 Clone # 903502
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human SLC5A8/SMCT1
Met1-Leu610
Accession # NP_666018
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of SLC5A8/SMCT1 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of SLC5A8/SMCT1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human SLC5A8/SMCT1 APC-conjugated Monoclonal Antibody (Catalog # FAB8398A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of SLC5A8/SMCT1 antibody in HEK293 Human Cell Line Transfected with Human SLC5A8/SMCT1 and eGFP antibody by Flow Cytometry. View Larger

Detection of SLC5A8/SMCT1 in HEK293 Human Cell Line Transfected with Human SLC5A8/SMCT1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human SLC5A8/SMCT1 and eGFP was stained with either (A) Mouse Anti-Human SLC5A8/SMCT1 APC-conjugated Monoclonal Antibody (Catalog # FAB8398A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SLC5A8/SMCT1

SLC5A8/SMCT1 (Solute Carrier family 5, member 8) is a member of the solute carrier family of multi-pass membrane proteins. SLC5A8 has been shown to transport iodide by a passive mechanism. It also transports monocarboxylates and short-chain fatty acids by a sodium-coupled mechanism. SLC5A8 nuclear translocation and loss of expression are associated with poor outcome in pancreatic ductal adenocarcinoma (1). It may be responsible for the absorption of D-lactate and monocarboxylate drugs from the intestinal tract.

References
  1. Helm, J. et al. (2012) Pancreas 41:904.
Long Name
Solute Carrier Family 5 Iodide Transporter), Member 8
Entrez Gene IDs
160728 (Human); 216225 (Mouse); 500820 (Rat)
Alternate Names
AIT; AITSolute carrier family 5 member 8; Apical iodide transporter; Electrogenic sodium monocarboxylate cotransporter; MGC125354; SLC5A8; SMCT; SMCTSMCT1; Sodium iodide-related cotransporter; sodium-coupled monocarboxylate transporter 1; solute carrier family 5 (iodide transporter), member 8

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