Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Bioassay, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 20409
View all SLPI Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SLPI
Accession # P03973

Specificity

Detects recombinant human SLPI in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant mouse SLPI is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human SLPI Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human SLPI (Catalog # 1274-PI) under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLPI

SLPI is a member of the Serpin family and is primarily expressed in secretory glandular epithelial cells. It is found in various secretory fluids and functions as a potent inhibitor of many proteolytic enzymes. SLPI can modulate cell proliferation and has been shown to have anti-inflammatory, anti-viral, and anti-bacterial activities (1, 2).

References

  1. Zhang, D. et al. (2002) J. Biol. Chem. 277:29999.
  2. Taggart, C.C. et al. (2002) J. Biol. Chem. 277:33648.

Long Name

Secretory Leukocyte Protease Inhibitor

Alternate Names

ALK1, ALPProtease inhibitor WAP4, antileukoproteinase, BLPISeminal proteinase inhibitor, HUSI, HUSI-I, Mucus proteinase inhibitor, secretory leukocyte peptidase inhibitor, Secretory leukocyte protease inhibitor, secretory leukocyte protease inhibitor (antileukoproteinase), WAP four-disulfide core domain protein 4, WAP4HUSI-1, WFDC4MPI

Entrez Gene IDs

6590 (Human); 20568 (Mouse)

Gene Symbol

SLPI

UniProt

P03973

Additional SLPI Products

Product Documents for Human SLPI Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human SLPI Antibody

For research use only

Citations for Human SLPI Antibody

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