Human SMPD1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human SMPD1 by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Human SMPD1 Antibody Summary

Species Reactivity
Detects human SMPD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse SMPD1 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant human SMPD1
Accession # NP_000534
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
Human brain (cerebellum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SMPD1 antibody by Western Blot. View Larger

Detection of Human SMPD1 by Western Blot. Western blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SMPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5348) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SMPD1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western View Larger

Detection of Human SMPD1 by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum), loaded at 0.2 mg/mL. A specific band was detected for SMPD1 at approximately 87 kDa (as indicated) using 50 µg/mL of Goat Anti-Human SMPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5348). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMPD1

Sphingomyelin phosphodiesterase, also known as acid sphingomyelinase and encoded by the SMPD1 gene, is a lysosomal phosphodiesterase which belongs to the acid sphingomyelinase family (1). SMPD1 catalyzes the hydrolysis of sphingomyelin to ceramide and phosphorylcholine. Ceramide, a bioactive lipid, has emerged as an important signaling molecule involved in a variety of cellular processes such as cell differentiation, apoptosis, and proliferation (2). Activation of SMPD1 occurs by the removal, chemical modification or dimerization of its C-terminal cysteine residue (3). Deficiencies of SMPD1 result in a lysosomal storage disorder referred to as Niemann-Pick disease (4). rhSMPD1 was expressed without the last three C-terminal residues, and is therefore constitutively active.

  1. Schuchman, E.H. et al. (1991) J. Biol. Chem. 266:8531.
  2. Melendez, A.J. et al. (2008) Biochim. Biophys. Acta 1784:66.
  3. Qiu, H. et al. (2003) J. Biol. Chem. 278:32744.
  4. Smith, E.L. and Schuchman, E.H. (2008) FASEB J. 22:3419.
Long Name
Sphingomyelin Phosphodiesterase 1, Acid Lysosomal
Entrez Gene IDs
6609 (Human); 20597 (Mouse); 308909 (Rat)
Alternate Names
Acid sphingomyelinase; aSMase; ASMsphingomyelin phosphodiesterase; EC; NPD; SMPD1; sphingomyelin phosphodiesterase 1, acid lysosomal

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Citation for Human SMPD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Enrichment of NPC1-deficient cells with the lipid LBPA stimulates autophagy, improves lysosomal function, and reduces cholesterol storage
    Authors: O Ilnytska, K Lai, K Gorshkov, ML Schultz, BN Tran, M Jeziorek, TJ Kunkel, RD Azaria, HS McLoughlin, M Waghalter, Y Xu, M Schlame, N Altan-Bonn, W Zheng, AP Lieberman, R Dobrowolsk, J Storch
    The Journal of Biological Chemistry, 2021;0(0):100813.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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