Detection of Human SOCS‑5 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SOCS‑5 Monoclonal Antibody (Catalog # MAB4796) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS‑5 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SOCS-5 (Suppressor of cytokine signaling 5; also CIS-6) is a 55‑60 kDa member of the SOCS family of proteins. It is an SH2-domain containing cytoplasmic adaptor protein expressed by Th1 cells that is known to reduce the expression of the EGF receptor and block signaling by the IL-4 R alpha chain. Human SOCS-5 is 536 amino acids (aa) in length. It contains one SH2 domain (aa 381‑476) and a SOCS box (aa 471‑520) that mediates interaction with E3 ubiquitin ligase complexes. Full-length SOCS-5 is 94% aa identical to mouse SOCS-5.
Suppressor Of Cytokine Signaling 5
Entrez Gene IDs:
9655 (Human); 56468 (Mouse); 500616 (Rat)
CIS6; CIS6Cytokine-inducible SH2 protein 6; CISH5; CISH6; CISH6CIS-6; KIAA0671Cytokine-inducible SH2-containing protein 5; SOCS5; SOCS-5; SOCS-5CISH5; suppressor of cytokine signaling 5
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