Detection of Human SOCS‑5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOCS‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4796) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SOCS‑5 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SOCS (suppressor of cytokine signaling) proteins, also called CIS (cytokine-inducible SH2-domain containing) proteins, are a family of eight adaptor molecules active in signaling pathways that regulate innate and adaptive immune responses. All SOCS contain a single SH2 domain and a SOCS box, which recruits E3 ubiquitin ligase complexes, thus enhancing destruction of the molecules bound by SOCS proteins. SOCS proteins influence macrophage and dendritic cell activation and are essential for T cell development and differentiation.
Suppressor Of Cytokine Signaling 5
Entrez Gene IDs:
9655 (Human); 56468 (Mouse); 500616 (Rat)
CIS6; CIS6Cytokine-inducible SH2 protein 6; CISH5; CISH6; CISH6CIS-6; KIAA0671Cytokine-inducible SH2-containing protein 5; SOCS5; SOCS-5CISH5; suppressor of cytokine signaling 5
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