Human SOCS-5 Antibody Summary
Accession # O75159
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human SOCS‑5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOCS-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4796) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SOCS-5 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
SOCS‑5 in Jurkat Human Cell Line. SOCS-5 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human SOCS-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4796) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
SOCS-5 (Suppressor of cytokine signaling 5; also CIS-6) is a 55‑60 kDa member of the SOCS family of proteins. It is an SH2-domain containing cytoplasmic adaptor protein expressed by Th1 cells that is known to reduce the expression of the EGF receptor and block signaling by the IL-4 R alpha chain. Human SOCS-5 is 536 amino acids (aa) in length. It contains one SH2 domain (aa 381‑476) and a SOCS box (aa 471‑520) that mediates interaction with E3 ubiquitin ligase complexes. Full-length SOCS-5 is 94% aa identical to mouse SOCS-5.
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