Human SOD1/Cu-Zn SOD Antibody

Catalog # Availability Size / Price Qty
MAB3418
MAB3418-SP
Detection of Human SOD1/ Cu‑Zn SOD by Western Blot.
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Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (2)

Human SOD1/Cu-Zn SOD Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOD1/Cu‑Zn SOD in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) SOD2 or rhSOD3 is observed.
Source
Monoclonal Mouse IgG2A Clone # 348808
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOD1/Cu‑Zn SOD
Met1-Gln154
Accession # P00441
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
15-25 µg/mL
See below
Knockout Validated
SOD1/Cu-Zn SOD is specifically detected in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. 
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SOD1/ Cu-Zn SOD antibody by Western Blot. View Larger

Detection of Human SOD1/ Cu‑Zn SOD by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human SOD1/Cu-Zn SOD Monoclonal Antibody (Catalog # MAB3418) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). For additional reference, recombinant human SOD1, SOD2, and SOD3 (1 ng) were included. A specific band for SOD1/Cu-Zn SOD was detected at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry SOD1/Cu-Zn SOD antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

SOD1/Cu‑Zn SOD in HeLa Human Cell Line. SOD1/Cu-Zn SOD was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human SOD1/Cu-Zn SOD Monoclonal Antibody (Catalog # MAB3418) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Knockout Validated View Larger

Western Blot Shows Human SOD1/Cu‑Zn SOD Specificity Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and SOD1/Cu‑Zn SOD knockout HeLa cell line (KO). Nitrocellulose membrane was probed with 0.5 µg/mL of Mouse Anti-Human SOD1/Cu‑Zn SOD Monoclonal Antibody (Catalog # MAB3418) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for SOD1/Cu‑Zn SOD at approximately 16 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOD1/Cu-Zn SOD

Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 1, SOD1, also known as Cu/Zn SOD, soluble SOD and IPO-A, is a soluble, cytoplasmic 16 kDa homodimer. Each SOD1 monomer binds one Cu2+ and one Zn2+ ion. Three isozymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD1 shares 23% and 27% sequence identity with SOD2 and SOD3, respectively. Mutations in SOD1 have been implicated as causes of familial amyotrophic lateral sclerosis (ALS). The ALS-causing mutations of SOD1 are scattered throughout the protein and provide no clear functional or structural clues to the underlying disease mechanism. The oligomerization hypothesis suggests that mutant SOD1 proteins become misfolded and consequently oligomerize into high molecular weight aggregates that result in the death of motor neurons. The oxidative damage hypothesis suggests that loss of function mutation in SOD1 protein results in the accumulation of cellular superoxide radical, leading to free radical-mediated damage, the release of cytochrome c, and apoptosis.

Long Name
Superoxide Dismutase-1
Entrez Gene IDs
6647 (Human); 20655 (Mouse); 24786 (Rat)
Alternate Names
ALS; ALS1; amyotrophic lateral sclerosis 1 (adult); Cu; Cu/Zn superoxide dismutase; CuZn SOD; Cu-Zn SOD; EC 1.15.1.1; homodimer; hSod1; indophenoloxidase A; Ipo1; IPOA; SOD; SOD, cytosolic; SOD, Soluble; SOD1; superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; superoxide dismutase 1, soluble; Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic

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Citations for Human SOD1/Cu-Zn SOD Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Pharmacological Bypass of Cockayne Syndrome B Function in Neuronal Differentiation.
    Authors: Wang Y, Jones-Tabah J, Chakravarty P et al.
    Cell Rep.
  2. Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1 (SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence
    Authors: Ayoubi, R;Alshafie, W;You, Z;Southern, K;McPherson, PS;Laflamme, C;
    F1000Research
    Species: Human
    Sample Types: Cell Lysates, Transfected Whole Cells, Whole Cells
    Applications: Western Blot, ICC, Immunoprecipitation
  3. Neurotrophic interactions between neurons and astrocytes following AAV1‐Rheb(S16H) transduction in the hippocampus in vivo
    Authors: Min‐Tae Jeon, Gyeong Joon Moon, Sehwan Kim, Minji Choi, Yong‐Seok Oh, Dong Woon Kim et al.
    British Journal of Pharmacology
  4. A Brainstem-Spinal Cord Inhibitory Circuit for Mechanical Pain Modulation by GABA and Enkephalins
    Authors: Amaury François, Sarah A. Low, Elizabeth I. Sypek, Amelia J. Christensen, Chaudy Sotoudeh, Kevin T. Beier et al.
    Neuron
  5. Activation of EGFR by small compounds through coupling the generation of hydrogen peroxide to stable dimerization of Cu/Zn SOD1.
    Authors: Sakanyan V, Hulin P, Alves de Sousa R, Silva V, Hambardzumyan A, Nedellec S, Tomasoni C, Loge C, Pineau C, Roussakis C, Fleury F, Artaud I
    Sci Rep, 2016-02-17;6(0):21088.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. An integrative proteomics method identifies a regulator of translation during stem cell maintenance and differentiation
    Authors: P Sabatier, CM Beusch, AA Saei, M Aoun, N Moruzzi, A Coelho, N Leijten, M Nordenskjö, P Micke, D Maltseva, AG Tonevitsky, V Millischer, J Carlos Vil, S Kadekar, M Gaetani, K Altynbekov, A Kel, PO Berggren, O Simonson, KH Grinnemo, R Holmdahl, S Rodin, RA Zubarev
    Nature Communications, 2021-11-12;12(1):6558.
  7. Activation of STAT3 Regulates Reactive Astrogliosis and Neuronal Death Induced by A beta O Neurotoxicity
    Authors: Toral-Rios D, PatiNo-LOpez G, GOmez-Lira G et al.
    Int J Mol Sci
  8. The c-Abl inhibitor, Radotinib HCl, is neuroprotective in a preclinical Parkinson's disease mouse model
    Authors: Lee S, Kim S, Park Y et al.
    Hum Mol Genet.

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Reviews for Human SOD1/Cu-Zn SOD Antibody

Average Rating: 4 (Based on 2 Reviews)

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Human SOD1/Cu-Zn SOD Antibody
By Anonymous on 03/17/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human SOD1/Cu-Zn SOD Antibody
By Juliana Redondo on 11/24/2016
Application: WB Sample Tested: Mesenchymal stem cells Species: Human

Used 1:2000 in milk (5% for blocking solution and 2% for antibody solution)