Human SOX17 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC1924A
Detection of SOX17 in Definitive Endoderm-differentiated BG01V Human Stem Cells by Flow Cytometry.
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Product Details
Citations (15)
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Human SOX17 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX2, rhSOX6, and rhSOX15 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of SOX17 antibody in Definitive Endoderm-differentiated BG01V Human Stem Cells antibody by Flow Cytometry. View Larger

Detection of SOX17 in Definitive Endoderm-differentiated BG01V Human Stem Cells by Flow Cytometry. Definitive endoderm-differentiated (Catalog # SC019) BG01V human embryonic stem cells were stained with Goat Anti-Human SOX17 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1924A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Flow Cytometry Detection of Human SOX17 by Flow Cytometry View Larger

Detection of Human SOX17 by Flow Cytometry Addition of DMSO to Activin A/low serum-based medium increases the efficiency of definitive endoderm differentiation.(A) Flow cytometry analysis of hESC and DE cells double-stained for the pluripotency marker OCT4 and the endoderm marker SOX17. Pluripotency and differentiation status in all culture conditions tested are shown as contour plots. Only upon addition of DMSO (0.5%, 1%) to Activin A-driven differentiation of hESC was the number of OCT4-positive cells significantly down regulated. Culture with 1% of DMSO yielded the highest number of SOX17+, OCT4- cells. ‘Null’ represents spontaneous differentiation with no differentiating growth factors/reagents added. Each contour plot represents 3 biological replicates pooled together. (B) Immunofluorescence staining of cells at day 2 of DE formation for OCT4 and SOX17 markers with DAPI nuclear staining. Cells cultured in Activin A alone were clearly separated into two widespread populations of SOX17- or OCT4-positive cells (B-A). DMSO in the absence of Activin A does not prime cells to acquire endoderm identity (B-B). Addition of 0.5% of DMSO to Activin A-based, DE-priming medium results in significant decrease of OCT4-positive clusters of cells as early as 48h after DE differentiation was initiated (B-C). Scale bar 100μm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0117689), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SOX17

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2). In Western blot, AF1924 detects a 60 kDa band of endogenous SOX17 which is higher than predicted molecular weight but consistent with the current literature (3).

 

References
  1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
  2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
  3. Sohn, J. et al. (2006) J. Neuroscience 26:9722.
Long Name
Transcription Factor SOX17
Entrez Gene IDs
64321 (Human)
Alternate Names
FLJ22252; SOX17; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3

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Citations for Human SOX17 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Chemically defined generation of human definitive hematopoietic stem and progenitor cells
    Authors: Juhyung Jung, Yun Chang, Gyuhyung Jin, Qing Deng, Xiaojun Lance Lian, Xiaoping Bao
    STAR Protocols
  2. Pulmonary fibrosis model using micro-CT analyzable human PSC–derived alveolar organoids containing alveolar macrophage-like cells
    Authors: Seri Choi, Jiwoong Choi, Seongmin Cheon, Jihong Song, Seung-Yeon Kim, Ji-eun Kim et al.
    Cell Biology and Toxicology
  3. Engineering chimeric antigen receptor neutrophils from human pluripotent stem cells for targeted cancer immunotherapy
    Authors: Yun Chang, Ramizah Syahirah, Xuepeng Wang, Gyuhyung Jin, Sandra Torregrosa-Allen, Bennett D. Elzey et al.
    Cell Reports
  4. Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification
    Authors: Grace A. Christopher, Rebecca J. Noort, Jessica L. Esseltine
    Biomolecules
  5. Repair of acute liver damage with immune evasive hESC derived hepato-blasts
    Authors: J Liu, T Pan, Y Chen, Y Liu, F Yang, Q Chen, N Abbas, M Zhong, Q Zhang, Y Xu, YX Li
    Stem Cell Res, 2020-09-26;49(0):102010.
    Species: Human, Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  6. Generation of pancreatic ? cells from CD177(+) anterior definitive endoderm
    Authors: Mahaddalkar PU, Scheibner K, Pfluger S et al.
    Nature Biotechnology
  7. Human iPSCs can be differentiated into notochordal cells that reduce intervertebral disc degeneration in a porcine model
    Authors: D Sheyn, S Ben-David, W Tawackoli, Z Zhou, K Salehi, M Bez, S De Mel, V Chan, J Roth, P Avalos, JC Giaconi, H Yameen, L Hazanov, D Seliktar, D Li, D Gazit, Z Gazit
    Theranostics, 2019-10-12;9(25):7506-7524.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Single-cell RNA sequencing reveals metallothionein heterogeneity during hESC differentiation to definitive endoderm
    Authors: J Lu, A Baccei, E Lummertz d, C Guillermie, S McManus, LA Finney, C Zhang, ML Steinhause, H Li, PH Lerou
    Stem Cell Res, 2018-01-31;28(0):48-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Mesendogen, a novel inhibitor of TRPM6, promotes mesoderm and definitive endoderm differentiation of human embryonic stem cells through alteration of magnesium homeostasis
    Authors: Yijie Geng, Bradley Feng
    Heliyon
  10. DMSO efficiently down regulates pluripotency genes in human embryonic stem cells during definitive endoderm derivation and increases the proficiency of hepatic differentiation.
    Authors: Czysz, Katherin, Minger, Stephen, Thomas, Nick
    PLoS ONE, 2015-02-06;10(2):e0117689.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells.
    Authors: Wong, Amy P, Chin, Stephani, Xia, Sunny, Garner, Jodi, Bear, Christin, Rossant, Janet
    Nat Protoc, 2015-02-05;10(3):363-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture.
    Authors: Takeuchi, Hiroki, Nakatsuji, Norio, Suemori, Hirofumi
    Sci Rep, 2014-03-27;4(0):4488.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Alternative functional in vitro models of human intestinal epithelia
    Authors: Amanda L. Kauffman, Alexandra V. Gyurdieva, John R. Mabus, Chrissa Ferguson, Zhengyin Yan, Pamela J. Hornby
    Frontiers in Pharmacology
  14. BRACHYURY and CDX2 mediate BMP-induced differentiation of human and mouse pluripotent stem cells into embryonic and extraembryonic lineages.
    Authors: Bernardo AS, Faial T, Gardner L, Niakan KK, Ortmann D, Senner CE, Callery EM, Trotter MW, Hemberger M, Smith JC, Bardwell L, Moffett A, Pedersen RA
    Cell Stem Cell, 2011-08-05;9(2):144-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency
    Authors: N Bredenkamp, J Yang, J Clarke, GG Stirparo, F von Meyenn, S Dietmann, D Baker, R Drummond, Y Ren, D Li, C Wu, M Rostovskay, S Eminli-Mei, A Smith, G Guo
    Stem Cell Reports, 2019-11-07;13(6):1083-1098.

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