Human SOX17 APC-conjugated Antibody
Human SOX17 APC-conjugated Antibody Summary
Asp177-Val414
Accession # Q9H6I2
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of SOX17 in Definitive Endoderm-differentiated BG01V Human Stem Cells by Flow Cytometry. Definitive endoderm-differentiated (Catalog # SC019) BG01V human embryonic stem cells were stained with Goat Anti-Human SOX17 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1924A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SOX17
SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2). In Western blot, AF1924 detects a 60 kDa band of endogenous SOX17 which is higher than predicted molecular weight but consistent with the current literature (3).
- Kanai-Azuma, M. et al. (2002) Development 129:2367.
- Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
- Sohn, J. et al. (2006) J. Neuroscience 26:9722.
Product Datasheets
Citations for Human SOX17 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Repair of acute liver damage with immune evasive hESC derived hepato-blasts
Authors: J Liu, T Pan, Y Chen, Y Liu, F Yang, Q Chen, N Abbas, M Zhong, Q Zhang, Y Xu, YX Li
Stem Cell Res, 2020;49(0):102010.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Human iPSCs can be differentiated into notochordal cells that reduce intervertebral disc degeneration in a porcine model
Authors: D Sheyn, S Ben-David, W Tawackoli, Z Zhou, K Salehi, M Bez, S De Mel, V Chan, J Roth, P Avalos, JC Giaconi, H Yameen, L Hazanov, D Seliktar, D Li, D Gazit, Z Gazit
Theranostics, 2019;9(25):7506-7524.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Single-cell RNA sequencing reveals metallothionein heterogeneity during hESC differentiation to definitive endoderm
Authors: J Lu, A Baccei, E Lummertz d, C Guillermie, S McManus, LA Finney, C Zhang, ML Steinhause, H Li, PH Lerou
Stem Cell Res, 2018;28(0):48-55.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells.
Authors: Wong, Amy P, Chin, Stephani, Xia, Sunny, Garner, Jodi, Bear, Christin, Rossant, Janet
Nat Protoc, 2015;10(3):363-81.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
DMSO efficiently down regulates pluripotency genes in human embryonic stem cells during definitive endoderm derivation and increases the proficiency of hepatic differentiation.
Authors: Czysz, Katherin, Minger, Stephen, Thomas, Nick
PLoS ONE, 2015;10(2):e0117689.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture.
Authors: Takeuchi, Hiroki, Nakatsuji, Norio, Suemori, Hirofumi
Sci Rep, 2014;4(0):4488.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
BRACHYURY and CDX2 mediate BMP-induced differentiation of human and mouse pluripotent stem cells into embryonic and extraembryonic lineages.
Authors: Bernardo AS, Faial T, Gardner L, Niakan KK, Ortmann D, Senner CE, Callery EM, Trotter MW, Hemberger M, Smith JC, Bardwell L, Moffett A, Pedersen RA
Cell Stem Cell, 2010;9(2):144-55.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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