Human SOX17 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC1924A
Human SOX17 APC-conjugated Antibody in Intracellular Staining by Flow Cytometry
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Product Details
Citations (6)
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Human SOX17 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX2, rhSOX6, and rhSOX15 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of SOX17 in Definitive Endoderm-differentiated BG01V Human Stem Cells by Flow Cytometry. View Larger

Detection of SOX17 in Definitive Endoderm-differentiated BG01V Human Stem Cells by Flow Cytometry. Definitive endoderm-differentiated (Catalog # SC019) BG01V human embryonic stem cells were stained with Goat Anti-Human SOX17 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1924A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SOX17

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2). In Western blot, AF1924 detects a 60 kDa band of endogenous SOX17 which is higher than predicted molecular weight but consistent with the current literature (3).

 

References
  1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
  2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
  3. Sohn, J. et al. (2006) J. Neuroscience 26:9722.
Long Name
Transcription Factor SOX17
Entrez Gene IDs
64321 (Human)
Alternate Names
FLJ22252; SOX17; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3

Product Datasheets

Citations for Human SOX17 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Human iPSCs can be differentiated into notochordal cells that reduce intervertebral disc degeneration in a porcine model
    Authors: D Sheyn, S Ben-David, W Tawackoli, Z Zhou, K Salehi, M Bez, S De Mel, V Chan, J Roth, P Avalos, JC Giaconi, H Yameen, L Hazanov, D Seliktar, D Li, D Gazit, Z Gazit
    Theranostics, 2019;9(25):7506-7524.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Single-cell RNA sequencing reveals metallothionein heterogeneity during hESC differentiation to definitive endoderm
    Authors: J Lu, A Baccei, E Lummertz d, C Guillermie, S McManus, LA Finney, C Zhang, ML Steinhause, H Li, PH Lerou
    Stem Cell Res, 2018;28(0):48-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells.
    Authors: Wong, Amy P, Chin, Stephani, Xia, Sunny, Garner, Jodi, Bear, Christin, Rossant, Janet
    Nat Protoc, 2015;10(3):363-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. DMSO efficiently down regulates pluripotency genes in human embryonic stem cells during definitive endoderm derivation and increases the proficiency of hepatic differentiation.
    Authors: Czysz, Katherin, Minger, Stephen, Thomas, Nick
    PLoS ONE, 2015;10(2):e0117689.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture.
    Authors: Takeuchi, Hiroki, Nakatsuji, Norio, Suemori, Hirofumi
    Sci Rep, 2014;4(0):4488.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. BRACHYURY and CDX2 mediate BMP-induced differentiation of human and mouse pluripotent stem cells into embryonic and extraembryonic lineages.
    Authors: Bernardo AS, Faial T, Gardner L, Niakan KK, Ortmann D, Senner CE, Callery EM, Trotter MW, Hemberger M, Smith JC, Bardwell L, Moffett A, Pedersen RA
    Cell Stem Cell, 2010;9(2):144-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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