Human SOX18 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human SOX18 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SOX18
    Ala276-Gly384
    Accession # P35713
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human SOX18 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HUVEC human umbilical vein endothelial cells, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human SOX18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5077) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SOX18 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX18

SOX18 (SRY-related HMG box) is a 41 kDa (predicted) Group F member of the SOX family of DNA-binding proteins. Human SOX18 is 384 amino acids (aa) in length and contains an N-terminal DNA-binding HMG box (aa 85‑153), a central TAD region (aa 168‑260) and a highly charged sequence that contains a nine aa transactivation motif (aa 323‑331). SOX18 is expressed by endothelium and vascular smooth muscle, and apparently promotes the formation and organization of new capillaries. Over aa 276‑384, human SOX18 shares 90% and 89% aa identity with mouse and porcine SOX18, respectively.

  • Long Name:
    SRY-related HMG-box 18
  • Entrez Gene IDs:
    54345 (Human); 20672 (Mouse); 311723 (Rat)
  • Alternate Names:
    HLTS; SOX18; SRY (sex determining region Y)-box 18; SRY-box 18; transcription factor SOX-18
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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