Human SPARC-like 1/SPARCL1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human SPARC‑like 1/SPARCL1 by Western Blot.
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Product Details
Citations (5)
Supplemental Products
Reviews (4)

Human SPARC-like 1/SPARCL1 Antibody Summary

Species Reactivity
Detects human SPARC-like 1/SPARCL1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse (rm) SPARC-like 1/SPARCL1 is observed and less than 1% cross-reactivity with recombinant human SPARC is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human SPARC-like 1/SPARCL1 (R&D Systems, Catalog # 2728-SL)
Accession # Q8N4S1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SPARC-like 1/SPARCL1 antibody by Western Blot. View Larger

Detection of Human SPARC‑like 1/SPARCL1 by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SPARC-like 1/ SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SPARC-like 1/SPARCL1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry SPARC-like 1/SPARCL1 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

SPARC‑like 1/SPARCL1 in Human Brain. SPARC-like 1/SPARCL1 was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human SPARC-like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Intracellular Staining by Flow Cytometry Detection of SPARC-like 1/SPARCL1 antibody in HL-60 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of SPARC‑like 1/SPARCL1 in HL‑60 Human Cell Line by Flow Cytometry. HL-60 human acute promyelocytic leukemia cell line was stained with Goat Anti-Human SPARC-like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SPARC-like 1/SPARCL1

SPARCL1 (Secreted Protein, Acidic and Rich in Cysteines-like 1), also known as hevin, SC1 or MAST9, is a member of the SPARC family of extracellular glycoproteins (1, 2). SPARCL1 is an anti-adhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver (3, 4). Human SPARCL1 contains a 16 amino acid (aa) signal sequence and a 648 aa mature region with four domains: a 416 aa N-terminal acidic region, a 23 aa follistatin-like domain, a 55 aa kazal-like segment and a 48 aa EF-hand/calcium-binding domain (3, 4). SPARCL1 is predicted at 75 kDa, but migrates at ~130 kDa, which has been explained either by disulfide-linked homodimerization or by glycosylation and high acidity (3-5). Some truncated forms have been reported. In mouse, a 55 kDa C-terminal fragment is the only form in kidney and represents a portion of SPARCL1 in other tissues (6). In humans, a 25 kDa form is increased in liver tumors that are encapsulated, while the full-length form is downregulated in many epithelial cell-derived tumors (7, 8). SPARCL1 inhibits adhesion and spreading on a variety of substrates (5, 9). It is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma (10). In tonsillar high endothelial venules (HEV), SPARCL1 may induce endothelial cell dissociation, promoting extravasation (3). SPARCL1 binds collagen; in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin (6, 11). Human mature SPARCL1 shares 67%, 69%, 78%, 76%, 72%, and 72% aa identity with mouse, rat, equine, canine, porcine and bovine SPARCL1, respectively. The follistatin-like, kazal-like and calcium-binding domains of SPARCL1 show 61% aa identity with corresponding regions of SPARC.

  1. Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
  2. Sullivan, M.M. and E.H. Sage (2004) Int. J. Biochem. Cell Biol. 36:991.
  3. Girard, J.P. and T.A. Springer (1995) Immunity 2:113.
  4. Bendik, I. et al. (1998) Cancer Res. 58:626.
  5. Brekken, R.A. et al. (2004) J. Histochem. Cytochem. 52:735.
  6. Hambrock, H.O. et al. (2003) J. Biol. Chem. 278:11351.
  7. Lau, C.P. et al. (2006) J. Pathol. 210:469.
  8. Isler, S.G. et al. (2001) Int. J. Oncol. 18:521.
  9. Girard, J.P. and T.A. Springer (1996) J. Biol. Chem. 271:4511.
  10. Gongidi, V. et al. (2004) Neuron 41:57.
  11. Sullivan, M.M. et al. (2006) J. Biol. Chem. 281:27621.
Entrez Gene IDs
8404 (Human); 13602 (Mouse)
Alternate Names
Hevin; High endothelial venule protein; MAST 9; MAST9; PIG33; proliferation-inducing protein 33; SC1; SPARC like 1; SPARCL1; SPARC-like 1 (hevin); SPARC-like 1 (mast9, hevin); SPARC-like 1; SPARC-like protein 1

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Citations for Human SPARC-like 1/SPARCL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Heterogeneity of glial progenitor cells during the neurogenesis-to-gliogenesis switch in the developing human cerebral cortex
    Authors: Y Fu, M Yang, H Yu, Y Wang, X Wu, J Yong, Y Mao, Y Cui, X Fan, L Wen, J Qiao, F Tang
    Cell Reports, 2021;34(9):108788.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Species-, organ- and cell-type-dependent expression of SPARCL1 in human and mouse tissues
    Authors: A Klingler, D Regensburg, C Tenkerian, N Britzen-La, A Hartmann, M Stürzl, E Naschberge
    PLoS ONE, 2020;15(5):e0233422.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. SPARCL1, a Novel Prognostic Predictive Factor for GI Malignancies: a Meta-Analysis
    Authors: H Hu, W Cai, S Zheng, W Ge
    Cell. Physiol. Biochem., 2017;44(4):1485-1496.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Down-regulated SPARCL1 is associated with clinical significance in human gastric cancer.
    Authors: Li P, Qian J, Yu G, Chen Y, Liu K, Li J, Wang J
    J Surg Oncol, 2012;105(1):31-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Molecular resistance fingerprint of pemetrexed and platinum in a long-term survivor of mesothelioma.
    Authors: Roe O, Szulkin A, Anderssen E, Flatberg A, Sandeck H, Amundsen T, Erlandsen S, Dobra K, Sundstrom S
    PLoS ONE, 2012;7(8):e40521.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P


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Reviews for Human SPARC-like 1/SPARCL1 Antibody

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Human SPARC-like 1/SPARCL1 Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human SPARC-like 1/SPARCL1 Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human SPARC-like 1/SPARCL1 Antibody
By Anonymous on 10/09/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human SPARC-like 1/SPARCL1 Antibody
By Min Hyung Kang on 10/05/2017
Application: WB Sample Tested: Trabecular meshwork tissue Species: Human