Human SPARC‑like 1/SPARCL1 Antibody

R&D Systems | Catalog # AF2728

R&D Systems
Discontinued Product
AF2728 has been discontinued. View all SPARC-like 1/SPARCL1 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry, FACS

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SPARC-like 1/SPARCL1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SPARC-like 1/SPARCL1 (R&D Systems, Catalog # 2728-SL)
Ile17-Phe664
Accession # Q8N4S1

Specificity

Detects human SPARC-like 1/SPARCL1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse (rm) SPARC-like 1/SPARCL1 is observed and less than 1% cross-reactivity with recombinant human SPARC is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SPARC‑like 1/SPARCL1 Antibody

Detection of Human SPARC-like 1/SPARCL1 antibody by Western Blot.

Detection of Human SPARC‑like 1/SPARCL1 by Western Blot.

Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SPARC-like 1/ SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SPARC-like 1/SPARCL1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SPARC-like 1/SPARCL1 antibody in Human Brain by Immunohistochemistry (IHC-P).

SPARC‑like 1/SPARCL1 in Human Brain.

SPARC-like 1/SPARCL1 was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human SPARC-like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of SPARC-like 1/SPARCL1 antibody in HL-60 Human Cell Line antibody by Flow Cytometry.

Detection of SPARC‑like 1/SPARCL1 in HL‑60 Human Cell Line by Flow Cytometry.

HL-60 human acute promyelocytic leukemia cell line was stained with Goat Anti-Human SPARC-like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human SPARC‑like 1/SPARCL1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample:

HL‑60 human acute promyelocytic leukemia cell line fixed with paraformaldehyde and permeabilized with saponin.

Western Blot

1 µg/mL
Sample: Human lung tissue

Reviewed Applications

Read 4 reviews rated 4.3 using AF2728 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: SPARC-like 1/SPARCL1

SPARCL1 (Secreted Protein, Acidic and Rich in Cysteines-like 1), also known as hevin, SC1 or MAST9, is a member of the SPARC family of extracellular glycoproteins (1, 2). SPARCL1 is an anti-adhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver (3, 4). Human SPARCL1 contains a 16 amino acid (aa) signal sequence and a 648 aa mature region with four domains: a 416 aa N-terminal acidic region, a 23 aa follistatin-like domain, a 55 aa kazal-like segment and a 48 aa EF-hand/calcium-binding domain (3, 4). SPARCL1 is predicted at 75 kDa, but migrates at ~130 kDa, which has been explained either by disulfide-linked homodimerization or by glycosylation and high acidity (3-5). Some truncated forms have been reported. In mouse, a 55 kDa C-terminal fragment is the only form in kidney and represents a portion of SPARCL1 in other tissues (6). In humans, a 25 kDa form is increased in liver tumors that are encapsulated, while the full-length form is downregulated in many epithelial cell-derived tumors (7, 8). SPARCL1 inhibits adhesion and spreading on a variety of substrates (5, 9). It is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma (10). In tonsillar high endothelial venules (HEV), SPARCL1 may induce endothelial cell dissociation, promoting extravasation (3). SPARCL1 binds collagen; in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin (6, 11). Human mature SPARCL1 shares 67%, 69%, 78%, 76%, 72%, and 72% aa identity with mouse, rat, equine, canine, porcine and bovine SPARCL1, respectively. The follistatin-like, kazal-like and calcium-binding domains of SPARCL1 show 61% aa identity with corresponding regions of SPARC.

References

  1. Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
  2. Sullivan, M.M. and E.H. Sage (2004) Int. J. Biochem. Cell Biol. 36:991.
  3. Girard, J.P. and T.A. Springer (1995) Immunity 2:113.
  4. Bendik, I. et al. (1998) Cancer Res. 58:626.
  5. Brekken, R.A. et al. (2004) J. Histochem. Cytochem. 52:735.
  6. Hambrock, H.O. et al. (2003) J. Biol. Chem. 278:11351.
  7. Lau, C.P. et al. (2006) J. Pathol. 210:469.
  8. Isler, S.G. et al. (2001) Int. J. Oncol. 18:521.
  9. Girard, J.P. and T.A. Springer (1996) J. Biol. Chem. 271:4511.
  10. Gongidi, V. et al. (2004) Neuron 41:57.
  11. Sullivan, M.M. et al. (2006) J. Biol. Chem. 281:27621.

Alternate Names

Hevin, MAST9, SC1, SPARC like 1, SPARCL1

Entrez Gene IDs

8404 (Human); 13602 (Mouse)

Gene Symbol

SPARCL1

UniProt

Q8N4S1

Additional SPARC-like 1/SPARCL1 Products

Product Documents for Human SPARC‑like 1/SPARCL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human SPARC‑like 1/SPARCL1 Antibody

For research use only

Citations for Human SPARC‑like 1/SPARCL1 Antibody

Customer Reviews for Human SPARC‑like 1/SPARCL1 Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human SPARC‑like 1/SPARCL1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • Human SPARC-like 1/SPARCL1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human SPARC‑like 1/SPARCL1 Antibody AF2728
  • Human SPARC-like 1/SPARCL1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018
  • Human SPARC-like 1/SPARCL1 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 10/09/2018
  • Human SPARC-like 1/SPARCL1 Antibody
    Name: Min Hyung Kang
    Application: Western Blot
    Sample Tested: Trabecular meshwork tissue
    Species: Human
    Verified Customer | Posted 10/05/2017
    Human SPARC‑like 1/SPARCL1 Antibody AF2728

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies