Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all SPINK1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SPINK1
Asp24-Cys79
Accession # P00995

Specificity

Detects human SPINK1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human SPINK1 Antibody

Detection of Human SPINK1 antibody by Western Blot.

Detection of Human SPINK1 by Western Blot.

Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SPINK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7496) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPINK1 at approximately 6 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human SPINK1 antibody by Simple WesternTM.

Detection of Human SPINK1 by Simple WesternTM.

Simple Western lane view shows lysates of human pancreas tissue, loaded at 0.2 mg/mL. A specific band was detected for SPINK1 at approximately 5 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human SPINK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7496) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human SPINK1 antibody by Simple WesternTM.

Detection of Human SPINK1 by Simple WesternTM.

Simple Western lane view shows lysates of undifferentiated Ileum stem cells and day 12 differentiation Ileum stem cells, loaded at 0.2 mg/mL. A specific band was detected for SPINK1 at approximately 6 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human SPINK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7496) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Applications for Human SPINK1 Antibody

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: Human pancreas tissue and Day12 differentiation Ileum stem cells

Western Blot

0.5 µg/mL
Sample: Human pancreas tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SPINK1

SPINK1 (Serine Protease Inhibitor Kazal-type 1; also TATI and PST1) is a 6-7 kDa secreted polypeptide initially identified as a tumor-derived trypsin inhibitor. It is widely expressed, and found in cells diverse as pancreatic acinar cells, columnar cells of the stomach, renal collecting duct epithelium, and ureteric transitional plus breast epithelium. SPINK1 is known to be secreted with pancreatic zymogens, and apparently inactivates prematurely-activated trypsin, thus protecting the pancreas from trypsin-mediated enzyme activation. It also is reported to regulate cell migration and proliferation, the latter effect attributed to its structural resemblance to EGF, and its ability to bind to activate the EGFR. Mature human SPINK1 is 56 amino acids (aa) in length (aa 24-79). It contains one Kazal-like domain (aa 26-79) that possesses a potential proteolytic cleavage site between Lys41-Ile42. An 11-12 kDa form in SDS-PAGE has been reported for SPINK1, possibly reflecting dimerization. Mature human SPINK1 shares 66% aa sequence identity with mouse Spink3, the mouse equivalent to human SPINK1.

Long Name

Serine Peptidase Inhibitor, Kazal Type 1

Alternate Names

PCTT, PSTI, Spink3, TATI

Entrez Gene IDs

6690 (Human); 20730 (Mouse); 266602 (Rat)

Gene Symbol

SPINK1

UniProt

P00995

Additional SPINK1 Products

Product Documents for Human SPINK1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SPINK1 Antibody

For research use only

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