Human ST2/IL-33 R DuoSet ELISA

  (13 citations)
(2 Reviews)
    
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Citations (13)
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    • Assay Type
      Solid Phase Sandwich ELISA
    • Format
      96-well strip plate
    • Sample Volume Required
      100 µL
    • Sufficient Materials
      For five 96-well plates*
    • Specificity
      Please see the product datasheet
    * Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
    Ancillary Reagent Kit Available
    DY008 , DuoSet ELISA Ancillary Reagent Kit 2 - Please Inquire
    This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human ST2 / IL-33 R. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

    Product Features
    • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
    • Development protocols are provided to guide further assay optimization
    • Assay can be customized to your specific needs
    • Economical alternative to complete kits
    Kit Content
    • Capture Antibody
    • Detection Antibody
    • Recombinant Standard
    • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
    Other Reagents Required

    PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

    Wash Buffer: (Catalog # WA126), or equivalent

    Reagent Diluent*

    Blocking Buffer*

    Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

    Stop Solution: 2 N H2SO4 (Catalog # DY994)

    Microplates: R&D Systems (Catalog # DY990), or equivalent

    Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

    *For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

    Product Datasheets

    Certificate of Analysis Lookup

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Certificate of Analysis Lookup

    Certificate of Analysis Request Form

    To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Preparation and Storage
    • Stability & Storage
      Store the unopened product at 2 - 8 °C. Do not use past expiration date.
    Background: ST2/IL-33 R
    ST2, also known as IL-1 R4, is a component of the receptor for the proinflammatory cytokine Interleukin-33. The IL-33 receptor additionally includes IL-1 RAcp which also associates with IL-1 RI and IL-1 R rp2/IL-1 R6. A soluble form of ST2 functions as a decoy receptor. IL-33 promotes Th2-biased immune responses, resulting in eosinophilia, mast cell activation, and allergic inflammation. In addition, IL-33 enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. Soluble ST2 is elevated in the serum under inflammatory conditions including allergic asthma, sepsis, trauma, dengue fever, pulmonary disease, and lupus. It is also associated aortic stenosis, congestive cardiomyopathy, and risk of cardiovascular heart failure and death.
    • Long Name:
      Interleukin 33 Receptor
    • Entrez Gene IDs:
      9173 (Human); 17082 (Mouse)
    • Alternate Names:
      DER4; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33R; Ly84; ST2; ST2L; ST2V; suppression of tumorigenicity 2; T1
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Sample Type
  1. Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
    Authors: LM Scott, AH Bryant, A Rees, B Down, RH Jones, CA Thornton
    Cytokine, 2017;0(0):.
    Species: Human
    Sample Type: Tissue Culture Supernates
  2. Soluble plasma proteins ST2 and CD163 as early biomarkers of nephropathy in Swedish patients with diabetes, 15-34�years of age: a prospective cohort study
    Authors: M Samuelsson, J Dereke, MK Svensson, M Landin-Ols, M Hillman, on the beh
    Diabetol Metab Syndr, 2017;9(0):41.
    Species: Human
    Sample Type: Plasma
  3. A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis
    Authors: D Díaz-Jimén, L Núñez, M De la Fuen, K Dubois-Cam, H Sepúlveda, M Montecino, A Torres-Riq, P García-Gon, J Chnaiderma, A Vossenkamp, TT MacDonald, D Simian, MJ González, JA Cidlowski, R Quera, MA Hermoso
    Sci Rep, 2017;7(1):10180.
    Species: Human
    Sample Type: Serum
  4. Predictive value of low interleukin-33 in critically ill patients
    Authors: KA Krychtiuk, S Stojkovic, M Lenz, M Brekalo, K Huber, J Wojta, G Heinz, S Demyanets, WS Speidl
    Cytokine, 2017;0(0):.
    Species: Human
    Sample Type: Serum
  5. Aqueous two-phase system patterning of detection antibody solutions for cross-reaction-free multiplex ELISA.
    Authors: Frampton, John P, White, Joshua B, Simon, Arlyne B, Tsuei, Michael, Paczesny, Sophie, Takayama, Shuichi
    Sci Rep, 2014;4(0):4878.
    Species: Human
    Sample Type: Plasma
  6. Soluble ST2 Associates with Diabetes but Not Established Cardiovascular Risk Factors: A New Inflammatory Pathway of Relevance to Diabetes?
    Authors: Miller A, Purves D, McConnachie A, Asquith D, Batty G, Burns H, Cavanagh J, Ford I, McLean J, Packard C, Shiels P, Turner H, Velupillai Y, Deans K, Welsh P, McInnes I, Sattar N
    PLoS ONE, 2012;7(10):e47830.
    Species: Human
    Sample Type: Plasma
  7. Daily exposure to dust alters innate immunity.
    Authors: Sahlander K, Larsson K, Palmberg L
    PLoS ONE, 2012;7(2):e31646.
    Species: Human
    Sample Type: Serum
  8. Soluble IL-1RII and IL-18 are associated with incipient upper extremity soft tissue disorders.
    Authors: Rechardt M, Shiri R, Matikainen S, Viikari-Juntura E, Karppinen J, Alenius H
    Cytokine, 2011;54(2):149-53.
    Species: Human
    Sample Type: Serum
  9. Serum soluble ST2: a potential novel mediator in left ventricular and infarct remodeling after acute myocardial infarction.
    Authors: Weir RA, Miller AM, Murphy GE, Clements S, Steedman T, Connell JM, McInnes IB, Dargie HJ, McMurray JJ
    J. Am. Coll. Cardiol., 2010;55(3):243-50.
    Species: Human
    Sample Type: Serum
  10. Primary sources and immunological prerequisites for sST2 secretion in humans.
    Authors: Mildner M, Storka A, Lichtenauer M
    Cardiovasc. Res., 2010;87(4):769-77.
    Species: Human
    Sample Type: Cell Culture Supernates
  11. Potential role of soluble ST2 protein in idiopathic nephrotic syndrome recurrence following kidney transplantation.
    Authors: Bruneau S, Le Berre L, Herve C, Valanciute A, Kamal M, Naulet J, Tesson L, Foucher Y, Soulillou JP, Sahali D, Dantal J
    Am. J. Kidney Dis., 2009;54(3):522-32.
    Species: Human
    Sample Type: Serum
  12. Induction of IRAK-M is associated with lipopolysaccharide tolerance in a human endotoxemia model.
    Authors: van 't Veer C, van den Pangaart PS, van Zoelen MA, de Kruif M, Birjmohun RS, Stroes ES, de Vos AF, van der Poll T
    J. Immunol., 2007;179(10):7110-20.
    Species: Human
    Sample Type: Plasma
  13. Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
    Authors: Hong YS, Moon S, Moon SJ, Joo YB, Joo Y, Jeon C, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK
    J Korean Med Sci, 0;26(9):1132-9.
    Species: Human
    Sample Type: Serum
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Average Rating: 4.5 (Based on 2 reviews)

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 Human ST2/IL-33 R DuoSet ELISA DY523B-05 Array DY523B-05
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Excellent
  Anonymous 08/09/2017
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 Human ST2/IL-33 R DuoSet ELISA DY523B-05
: Human ST2/IL-33 R DuoSet ELISA [DY523B-05].

Summary

Sample Tested EDTA Plasma

Other Experimental Details

Other Experimental Details We ran this ELISA with human plasma samples at a 1:20 dilution reaching high precision.
 Human ST2/IL-33 R DuoSet ELISA DY523B-05 Array DY523B-05
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Very Good
  Anonymous 05/08/2017
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 Human ST2/IL-33 R DuoSet ELISA DY523B-05
: Human ST2/IL-33 R DuoSet ELISA [DY523B-05].

Summary

Sample Tested HUMAN SERUM

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