Home / ST6GALNAC4 / Human ST6GALNAC4 Antibody

Human ST6GALNAC4 Antibody

Catalog #: AF6876 Datasheet / COA / SDS

Discontinued Product

AF6876 has been discontinued.
View all ST6GALNAC4 products.
ST6GALNAC4 in MCF‑7 Human Cell Line.
1 Image
Product Details
FAQs
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ST6GALNAC4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ST6GALNAC4 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human ST6GALNAC5 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ST6GALNAC4
Thr36-Arg302
Accession # Q9H4F1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry ST6GALNAC4 antibody in MCF‑7 Human Cell Line by Immunocytochemistry (ICC). View Larger

ST6GALNAC4 in MCF‑7 Human Cell Line. ST6GALNAC4 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human ST6GALNAC4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6876) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST6GALNAC4

ST6GALNAC4 is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates (1). This enzyme works on both glycoproteins and glycolipids and has strict substrate specificity, utilizing only the trisaccharide sequence Neu5Ac alpha 2-3Gal beta 1-3GalNAc of glycoproteins and glycolipids (2). In particular, this enzyme is involved in the synthesis of ganglioside GD1A from GM1B (3). GD1A is the target pathogenic antigen in the autoimmune disease, Guillain-Barre Syndrome (4, 5). The activity of this enzyme has been measured using a phosphatase-coupled method (6).

References
  1. Harduin-Lepers, A. et al. (2005) Glycobiology 15:805.
  2. Harduin-Lepers, A. et al. (2000) Biochem. J. 352:37.
  3. Lee, Y. C. et al. (1999) J. Biol. Chem. 274:11958.
  4. Kusunoki, S. et al. (1994) Ann. Neurol. 35:570.
  5. Goodfellow, J.A. et al. (2005) J. Neurosci. 25:1620.
  6. Wu, Z.L. et al. (2011) Glycobiology 21:727.
Entrez Gene IDs
27090 (Human); 20448 (Mouse)
Alternate Names
EC 2.4.99; EC 2.4.99.7; NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc-alpha-2,6-sialyltransferase IV; NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc-alpha-2,6-sialyltransferase; Sialyltransferase 3C; Sialyltransferase 7D; sialyltransferase 7D((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase); SIAT3C; SIAT3-C; SIAT3C6-sialyltransferasealpha2,6-sialyltransferase; SIAT7D; SIAT7-D; ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 4; ST6GalNAc IV; ST6GALNAC4; ST6GalNAcIV

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human ST6GALNAC4 Antibody

There are currently no reviews for this product. Be the first to review Human ST6GALNAC4 Antibody and earn rewards!

Have you used Human ST6GALNAC4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review