Human sTfR Quantikine IVD ELISA Kit

For In Vitro Diagnostic Use
  (22 citations)
(1 Review)
    
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (22)
FAQs
Reviews
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    2.5 hours
  • Sample Type & Volume Required Per Well
    Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Citrate Plasma (20 uL)
  • Sensitivity
    0.5 nmol/L
  • Assay Range
    3.0 - 80 nmol/L (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Measures human sTfR levels in various samples
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    Interference observed with other substances.
Product Summary
The Quantikine IVD Human sTfR Immunoassay is designed for the quantitative determination of soluble transferrin receptor (sTfR) concentrations in human serum and plasma as an aid in the diagnosis of Iron-Deficiency Anemia (IDA), especially for the differential diagnosis of IDA and Anemia of Chronic Disease (ACD). For In Vitro Diagnostic Use.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 60 60 60
Mean 6.9 18.2 40.2 10.9 25.7 67.2
Standard Deviation 0.3 1.3 2.5 0.7 1.4 3.8
CV% 4.3 7.1 6.2 6.4 5.4 5.7

Recovery

The recovery of sTfR spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Citrate Plasma (n=5) 112 105-121
EDTA Plasma (n=5) 103 97-112
Heparin Plasma (n=5) 106 101-112
Serum (n=5) 97 95-100
Linearity
To assess the linearity of the assay, five samples containing high concentrations of sTfR were diluted with Specimen Diluent and evaluated.
 TfR (Transferrin R) [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TfR (Transferrin R)
Transferrin receptors are transmembrane, homodimeric proteins that play a role in iron transport, uptake, and storage. TfR is an essential receptor, found on almost all cell types, regulated by intracellular iron concentration, and has a high affiinity for transferrin. Soluble TfR (sTfR) arises from the proteolysis of TfR into monomers that can be measured in plasma and serum. TfR2 has limited tissue distribution (hepatocytes and enterocytes), is regulated by the cell cycle, and binds transferrin poorly.
  • Long Name:
    Transferrin Receptor
  • Entrez Gene IDs:
    7037 (Human); 22042 (Mouse); 64678 (Rat)
  • Alternate Names:
    CD71 antigen; CD71; CD71TRFR; p90; T9; TfR (Transferrin R); TfR (TransferrinR); TfR; TFR1; TFRC; TR; transferrin receptor (p90, CD71); transferrin receptor protein 1; TRFR
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 20 µL Standard, Control, or Sample
  6.   Add 20 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  10.   Aspirate and wash 4 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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Species
Sample Type
  1. Hepcidin detects iron deficiency in Sri Lankan adolescents with a high burden of hemoglobinopathy: A diagnostic test accuracy study
    Authors: K Wray, A Allen, E Evans, C Fisher, A Premawardh, L Perera, R Rodrigo, G Goonathila, L Ramees, C Webster, AE Armitage, AM Prentice, DJ Weatherall, H Drakesmith, SR Pasricha
    Am. J. Hematol, 2017;92(2):196-203.
    Species: Human
    Sample Type: Serum
  2. Fibroblast growth factor 23, iron and inflammation - are they related in early stages of chronic kidney disease?
    Authors: E Lukaszyk, M Lukaszyk, E Koc-Zoraws, A Bodzenta-L, J Malyszko
    Arch Med Sci, 2017;13(4):845-850.
    Species: Human
    Sample Type: Serum
  3. The impact of maternal obesity on iron status, placental transferrin receptor expression and hepcidin expression in human pregnancy.
    Authors: Garcia-Valdes L, Campoy C, Hayes H, Florido J, Rusanova I, Miranda M, McArdle H
    Int J Obes (Lond), 2015;39(4):571-8.
    Species: Human
    Sample Type: Serum
  4. Host iron redistribution as a risk factor for incident tuberculosis in HIV infection: an 11-year retrospective cohort study.
    Authors: McDermid, Joann M, Hennig, Branwen, van der Sande, Marianne, Hill, Adrian V, Whittle, Hilton C, Jaye, Assan, Prentice, Andrew M
    BMC Infect Dis, 2013;13(0):48.
    Species: Human
    Sample Type: Plasma
  5. Regulation of hepcidin expression at high altitude.
    Authors: Talbot NP, Lakhal S, Smith TG, Privat C, Nickol AH, Rivera-Ch M, Leon-Velarde F, Dorrington KL, Mole DR, Robbins PA
    Blood, 2012;119(3):857-60.
    Species: Human
    Sample Type: Plasma
  6. Modulation of hepcidin production during hypoxia-induced erythropoiesis in humans in vivo: data from the HIGHCARE project.
    Authors: Piperno A, Galimberti S, Mariani R
    Blood, 2011;117(10):2953-9.
    Species: Human
    Sample Type: Serum
  7. Comparison between one and three doses a week of recombinant erythropoietin in very low birth weight infants.
    Authors: Vazquez Lopez MA, Llamas MA, Galera R, Sanchez AR, Lendinez F, Gonzalez-Ripoll M, Diez J, Bonillo A
    J Perinatol, 2011;31(0):118-24.
    Species: Human
    Sample Type: Serum
  8. Randomized, double-blind, placebo-controlled trial of an iron-fortified food product in female soldiers during military training: relations between iron status, serum hepcidin, and inflammation.
    Authors: Karl JP, Lieberman HR, Cable SJ, Williams KW, Young AJ, McClung JP
    Am. J. Clin. Nutr., 2010;92(1):93-100.
    Species: Human
    Sample Type: Serum
  9. Mortality in HIV infection is independently predicted by host iron status and SLC11A1 and HP genotypes, with new evidence of a gene-nutrient interaction.
    Authors: McDermid JM, van der Loeff MF, Jaye A, Hennig BJ, Bates C, Todd J, Sirugo G, Hill AV, Whittle HC, Prentice AM
    Am. J. Clin. Nutr., 2009;90(1):225-33.
    Species: Human
    Sample Type: Plasma
  10. Randomized, double-blind, placebo-controlled trial of iron supplementation in female soldiers during military training: effects on iron status, physical performance, and mood.
    Authors: McClung JP, Karl JP, Cable SJ, Williams KW, Nindl BC, Young AJ, Lieberman HR
    Am. J. Clin. Nutr., 2009;90(1):124-31.
    Species: Human
    Sample Type: Serum
  11. Body iron excretion by healthy men and women.
    Authors: Hunt JR, Zito CA, Johnson LK
    Am. J. Clin. Nutr., 2009;89(6):1792-8.
    Species: Human
    Sample Type: Serum
  12. Time course of anemia during six months follow up following intensive care discharge and factors associated with impaired recovery of erythropoiesis.
    Authors: Bateman AP, McArdle F, Walsh TS
    Crit. Care Med., 2009;37(6):1906-12.
    Species: Human
    Sample Type: Serum
  13. Excessive body iron stores are not associated with risk of coronary heart disease in women.
    Authors: Sun Q, Ma J, Rifai N, Franco OH, Rexrode KM, Hu FB
    J. Nutr., 2008;138(12):2436-41.
    Species: Human
    Sample Type: Plasma
  14. Tumor necrosis factor SNP haplotypes are associated with iron deficiency anemia in West African children.
    Authors: Atkinson SH, Rockett KA, Morgan G, Bejon PA, Sirugo G, O'Connell MA, Hanchard N, Kwiatkowski DP, Prentice AM
    Blood, 2008;112(10):4276-83.
    Species: Human
    Sample Type: Plasma
  15. Retinol and riboflavin supplementation decreases the prevalence of anemia in chinese pregnant women taking iron and folic Acid supplements.
    Authors: Ma AG, Schouten EG, Zhang FZ, Kok FJ, Yang F, Jiang DC, Sun YY, Han XX
    J. Nutr., 2008;138(10):1946-50.
    Species: Human
    Sample Type: Plasma
  16. Lung injury after ozone exposure is iron dependent.
    Authors: Ghio AJ, Turi JL, Madden MC, Dailey LA, Richards JD, Stonehuerner JG, Morgan DL, Singleton S, Garrick LM, Garrick MD
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(1):L134-43.
    Species: Human
    Sample Type: BALF
  17. Electrolytic iron or ferrous sulfate increase body iron in women with moderate to low iron stores.
    Authors: Swain JH, Johnson LK, Hunt JR
    J. Nutr., 2007;137(3):620-7.
    Species: Human
    Sample Type: Serum
  18. High levels of GDF15 in thalassemia suppress expression of the iron regulatory protein hepcidin.
    Authors: Tanno T, Bhanu NV, Oneal PA, Goh SH, Staker P, Lee YT, Moroney JW, Reed CH, Luban NL, Wang RH, Eling TE, Childs R, Ganz T, Leitman SF, Fucharoen S, Miller JL
    Nat. Med., 2007;13(9):1096-101.
    Species: Human
    Sample Type: Serum
  19. Association between transferrin receptor-ferritin index and conventional measures of iron responsiveness in hemodialysis patients.
    Authors: Chen YC, Hung SC, Tarng DC
    Am. J. Kidney Dis., 2006;47(6):1036-44.
    Species: Human
    Sample Type: Serum
  20. Evaluation of tumor necrosis factor-alpha and erythropoietin serum levels in B-cell chronic lymphocytic leukemia patients with anemia.
    Authors: Capalbo S, Battista C, Delia M, Ciancio A, De Santis G, Dargenio M, Diomede D, Liso V
    Acta Haematol., 2002;108(2):84-9.
    Species: Human
    Sample Type: Serum
  21. Clinical and laboratory manifestations of congenital dyserythropoietic anemia type I in young adults.
    Authors: Shalev H, Kapleushnik Y, Haeskelzon L, Degani O, Kransnov T, Sphilberg O, Moser A, Yaniv I, Tamary H
    Eur. J. Haematol., 2002;68(3):170-4.
    Species: Human
    Sample Type: Serum
  22. Erythroblast iron metabolism and serum soluble transferrin receptor values in the anemia of rheumatoid arthritis.
    Authors: Fitzsimons EJ, Houston T, Munro R, Sturrock RD, Speekenbrink AB, Brock JH
    Arthritis Rheum., 2002;47(2):166-71.
    Species: Human
    Sample Type: Serum
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