Transferrin Receptor (TfR, TfR-1, CD71) is a transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a disulfide-linked homodimer. As the major mediator of cellular iron uptake, it binds and internalizes diferric transferrin, allowing iron release at the low pH of the endosome. Proteolytic shedding of TfR into the circulation is enhanced by iron deficiency. The hereditary hemochromatosis protein HFE competes with diferric transferrin for binding to TfR and targets TfR for degradation rather than recycling. Cancer cells can express up to 5-fold more TfR than quiescent cells in the surrounding tissue. TfR carries out ferritin-independent functions in T cell development, immunological synapse formation and Galectin-3-mediated cell death, and it serves as a cell entry receptor for New World hemorrhagic fever arenaviruses.
Human sTfR Quantikine IVD ELISA Kit
R&D Systems | Catalog # DTFR1
Key Product Details
Assay Length
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Summary for Human sTfR Quantikine IVD ELISA Kit
Product Specifications
Assay Type
Format
Measurement
Detection Method
Conjugate
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 60 | 60 | 60 |
| Mean (nmol/L) | 6.9 | 18.2 | 40.2 | 10.9 | 25.7 | 67.2 |
| Standard Deviation | 0.3 | 1.3 | 2.5 | 0.7 | 1.4 | 3.8 |
| CV% | 4.3 | 7.1 | 6.2 | 6.4 | 5.4 | 5.7 |
Recovery for Human sTfR Quantikine IVD ELISA Kit
The recovery of sTfR spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Citrate Plasma (n=5) | 112 | 105-121 |
| EDTA Plasma (n=5) | 103 | 97-112 |
| Heparin Plasma (n=5) | 106 | 101-112 |
| Serum (n=5) | 97 | 95-100 |
Linearity
To assess the linearity of the assay, five samples containing high concentrations of sTfR were diluted with Specimen Diluent and evaluated.
Scientific Data Images for Human sTfR Quantikine IVD ELISA Kit
Human TfR (Transferrin R) In Vitro Diagnostic ELISA Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: TfR (Transferrin R)
Long Name
Alternate Names
Gene Symbol
Additional TfR (Transferrin R) Products
Product Documents for Human sTfR Quantikine IVD ELISA Kit
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Citations for Human sTfR Quantikine IVD ELISA Kit
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Protocols
View specific protocols for Human sTfR Quantikine IVD ELISA Kit (DTFR1):
Refer to the product datasheet for the complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 20 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
- Aspirate and wash 4 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
20 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
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