Human sTfR Quantikine IVD ELISA Kit

For In Vitro Diagnostic Use.
(21 citations)
(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    2.5 hours
  • Sample Type & Volume Required Per Well
    Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Citrate Plasma (20 uL)
  • Sensitivity
    0.5 nmol/L
  • Assay Range
    3.0 - 80 nmol/L (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Measures human sTfR levels in various samples
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    Interference observed with other substances.
Product Summary
The Quantikine IVD Human sTfR Immunoassay is designed for the quantitative determination of soluble transferrin receptor (sTfR) concentrations in human serum and plasma as an aid in the diagnosis of Iron-Deficiency Anemia (IDA), especially for the differential diagnosis of IDA and Anemia of Chronic Disease (ACD). For In Vitro Diagnostic Use.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020606060
Mean6.918.240.210.925.767.2
Standard Deviation0.31.32.50.71.43.8
CV%4.37.16.26.45.45.7

Recovery

The recovery of sTfR spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Citrate Plasma (n=5) 112 105-121
EDTA Plasma (n=5) 103 97-112
Heparin Plasma (n=5) 106 101-112
Serum (n=5) 97 95-100
Linearity
To assess the linearity of the assay, five samples containing high concentrations of sTfR were diluted with Specimen Diluent and evaluated.
Human sTfR Quantikine IVD ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TfR (Transferrin R)
Transferrin receptors are transmembrane, homodimeric proteins that play a role in iron transport, uptake, and storage. TfR is an essential receptor, found on almost all cell types, regulated by intracellular iron concentration, and has a high affiinity for transferrin. Soluble TfR (sTfR) arises from the proteolysis of TfR into monomers that can be measured in plasma and serum. TfR2 has limited tissue distribution (hepatocytes and enterocytes), is regulated by the cell cycle, and binds transferrin poorly.
    • Long Name
      Transferrin Receptor
    • Entrez Gene IDs
      7037 (Human); 22042 (Mouse); 64678 (Rat);
    • Alternate Names
      CD71 antigen; CD71; CD71TRFR; p90; T9; TfR (Transferrin R); TfR (TransferrinR); TfR; TFR1; TFRC; TR; transferrin receptor (p90, CD71); transferrin receptor protein 1; TRFR;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 20 µL Standard, Control, or Sample
    6.   Add 20 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    10.   Aspirate and wash 4 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    21 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Fibroblast growth factor 23, iron and inflammation - are they related in early stages of chronic kidney disease?
      Authors: E Lukaszyk, M Lukaszyk, E Koc-Zoraws, A Bodzenta-L, J Malyszko
      Arch Med Sci, 2017;13(4):845-850.
      Species: Human
      Sample Type: Serum
    2. The impact of maternal obesity on iron status, placental transferrin receptor expression and hepcidin expression in human pregnancy.
      Authors: Garcia-Valdes L, Campoy C, Hayes H, Florido J, Rusanova I, Miranda M, McArdle H
      Int J Obes (Lond), 2015;39(4):571-8.
      Species: Human
      Sample Type: Serum
    3. Host iron redistribution as a risk factor for incident tuberculosis in HIV infection: an 11-year retrospective cohort study.
      Authors: McDermid, Joann M, Hennig, Branwen, van der Sande, Marianne, Hill, Adrian V, Whittle, Hilton C, Jaye, Assan, Prentice, Andrew M
      BMC Infect Dis, 2013;13(0):48.
      Species: Human
      Sample Type: Plasma
    4. Regulation of hepcidin expression at high altitude.
      Authors: Talbot NP, Lakhal S, Smith TG, Privat C, Nickol AH, Rivera-Ch M, Leon-Velarde F, Dorrington KL, Mole DR, Robbins PA
      Blood, 2012;119(3):857-60.
      Species: Human
      Sample Type: Plasma
    5. Comparison between one and three doses a week of recombinant erythropoietin in very low birth weight infants.
      Authors: Vazquez Lopez MA, Llamas MA, Galera R, Sanchez AR, Lendinez F, Gonzalez-Ripoll M, Diez J, Bonillo A
      J Perinatol, 2011;31(0):118-24.
      Species: Human
      Sample Type: Serum
    6. Modulation of hepcidin production during hypoxia-induced erythropoiesis in humans in vivo: data from the HIGHCARE project.
      Authors: Piperno A, Galimberti S, Mariani R
      Blood, 2011;117(10):2953-9.
      Species: Human
      Sample Type: Serum
    7. Randomized, double-blind, placebo-controlled trial of an iron-fortified food product in female soldiers during military training: relations between iron status, serum hepcidin, and inflammation.
      Authors: Karl JP, Lieberman HR, Cable SJ, Williams KW, Young AJ, McClung JP
      Am. J. Clin. Nutr., 2010;92(1):93-100.
      Species: Human
      Sample Type: Serum
    8. Body iron excretion by healthy men and women.
      Authors: Hunt JR, Zito CA, Johnson LK
      Am. J. Clin. Nutr., 2009;89(6):1792-8.
      Species: Human
      Sample Type: Serum
    9. Time course of anemia during six months follow up following intensive care discharge and factors associated with impaired recovery of erythropoiesis.
      Authors: Bateman AP, McArdle F, Walsh TS
      Crit. Care Med., 2009;37(6):1906-12.
      Species: Human
      Sample Type: Serum
    10. Mortality in HIV infection is independently predicted by host iron status and SLC11A1 and HP genotypes, with new evidence of a gene-nutrient interaction.
      Authors: McDermid JM, van der Loeff MF, Jaye A, Hennig BJ, Bates C, Todd J, Sirugo G, Hill AV, Whittle HC, Prentice AM
      Am. J. Clin. Nutr., 2009;90(1):225-33.
      Species: Human
      Sample Type: Plasma
    11. Randomized, double-blind, placebo-controlled trial of iron supplementation in female soldiers during military training: effects on iron status, physical performance, and mood.
      Authors: McClung JP, Karl JP, Cable SJ, Williams KW, Nindl BC, Young AJ, Lieberman HR
      Am. J. Clin. Nutr., 2009;90(1):124-31.
      Species: Human
      Sample Type: Serum
    12. Retinol and riboflavin supplementation decreases the prevalence of anemia in chinese pregnant women taking iron and folic Acid supplements.
      Authors: Ma AG, Schouten EG, Zhang FZ, Kok FJ, Yang F, Jiang DC, Sun YY, Han XX
      J. Nutr., 2008;138(10):1946-50.
      Species: Human
      Sample Type: Plasma
    13. Tumor necrosis factor SNP haplotypes are associated with iron deficiency anemia in West African children.
      Authors: Atkinson SH, Rockett KA, Morgan G, Bejon PA, Sirugo G, O'Connell MA, Hanchard N, Kwiatkowski DP, Prentice AM
      Blood, 2008;112(10):4276-83.
      Species: Human
      Sample Type: Plasma
    14. Excessive body iron stores are not associated with risk of coronary heart disease in women.
      Authors: Sun Q, Ma J, Rifai N, Franco OH, Rexrode KM, Hu FB
      J. Nutr., 2008;138(12):2436-41.
      Species: Human
      Sample Type: Plasma
    15. Electrolytic iron or ferrous sulfate increase body iron in women with moderate to low iron stores.
      Authors: Swain JH, Johnson LK, Hunt JR
      J. Nutr., 2007;137(3):620-7.
      Species: Human
      Sample Type: Serum
    16. Lung injury after ozone exposure is iron dependent.
      Authors: Ghio AJ, Turi JL, Madden MC, Dailey LA, Richards JD, Stonehuerner JG, Morgan DL, Singleton S, Garrick LM, Garrick MD
      Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(1):L134-43.
      Species: Human
      Sample Type: BALF
    17. High levels of GDF15 in thalassemia suppress expression of the iron regulatory protein hepcidin.
      Authors: Tanno T, Bhanu NV, Oneal PA, Goh SH, Staker P, Lee YT, Moroney JW, Reed CH, Luban NL, Wang RH, Eling TE, Childs R, Ganz T, Leitman SF, Fucharoen S, Miller JL
      Nat. Med., 2007;13(9):1096-101.
      Species: Human
      Sample Type: Serum
    18. Association between transferrin receptor-ferritin index and conventional measures of iron responsiveness in hemodialysis patients.
      Authors: Chen YC, Hung SC, Tarng DC
      Am. J. Kidney Dis., 2006;47(6):1036-44.
      Species: Human
      Sample Type: Serum
    19. Clinical and laboratory manifestations of congenital dyserythropoietic anemia type I in young adults.
      Authors: Shalev H, Kapleushnik Y, Haeskelzon L, Degani O, Kransnov T, Sphilberg O, Moser A, Yaniv I, Tamary H
      Eur. J. Haematol., 2002;68(3):170-4.
      Species: Human
      Sample Type: Serum
    20. Evaluation of tumor necrosis factor-alpha and erythropoietin serum levels in B-cell chronic lymphocytic leukemia patients with anemia.
      Authors: Capalbo S, Battista C, Delia M, Ciancio A, De Santis G, Dargenio M, Diomede D, Liso V
      Acta Haematol., 2002;108(2):84-9.
      Species: Human
      Sample Type: Serum
    21. Erythroblast iron metabolism and serum soluble transferrin receptor values in the anemia of rheumatoid arthritis.
      Authors: Fitzsimons EJ, Houston T, Munro R, Sturrock RD, Speekenbrink AB, Brock JH
      Arthritis Rheum., 2002;47(2):166-71.
      Species: Human
      Sample Type: Serum
    Expand to show all 21 Citations
    Average Rating: 5 (Based on 1 review)

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