Human STING/TMEM173 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human STING/TMEM173 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human STING/TMEM173
    Ala215-Ser379
    Accession # Q86WV6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human STING/TMEM173 by Western Blot. Western blot shows lysates of human peripheral blood lymphocytes (PBL). PVDF Membrane was probed with 1 µg/mL of Human/Mouse STING/TMEM173 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6516) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for STING/TMEM173 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STING/TMEM173

STING (Stimulator of interferon genes; also ERIS, MPYS, MITA and TMEM173) is a 40-42 kDa 4-transmembrane protein that mediates both antiviral and MHC-II antigen recognition responses. STING is found in the ER, mitochondrial outer membrane and plasma membrane. It acts as an adaptor protein for intracellular viral detection molecules, participating in the induction of type I interferon. It also may play a role in the initiation of apoptosis following MHC-II engagement. Cells known to express STING include B cells, dendritic cells, macrophages, and monocytes. Human STING is 379 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-20), four transmembrane segments (aa 21-173), and a C-terminal cytoplasmic domain (aa 174-379). Ubiquitination occurs at Lys150, and phosphorylation occurs at Ser358. STING forms 80 kDa homodimers. There are two potential splice forms, one that shows a 25 aa substitution for aa 1-173, and another that possesses an alternative start site at Met215, coupled to a premature truncation following Arg334. Over aa 215-379, human STING shares 76% aa identity with mouse STING.

  • Long Name:
    Stimulator of Interferon Genes Protein/Transmembrane protein 173
  • Entrez Gene IDs:
    340061 (Human); 72512 (Mouse)
  • Alternate Names:
    endoplasmic reticulum IFN stimulator; Endoplasmic reticulum interferon stimulator; ERIS; FLJ38577; hMITA; Mediator of IRF3 activation; MITA; mitochondrial mediator of IRF3 activation; MPYS; NET23; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; Stimulator of interferon genes protein; STING; STINGhSTING; TMEM173; transmembrane protein 173
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. IFI16 is required for DNA sensing in human macrophages by promoting production and function of cGAMP
    Authors: KL Jønsson, A Laustsen, C Krapp, KA Skipper, K Thavachelv, D Hotter, JH Egedal, M Kjolby, P Mohammadi, T Prabakaran, LK Sørensen, C Sun, SB Jensen, CK Holm, RJ Lebbink, M Johannsen, M Nyegaard, JG Mikkelsen, F Kirchhoff, SR Paludan, MR Jakobsen
    Nat Commun, 2017;8(0):14391.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. cGAS Senses Human Cytomegalovirus and Induces Type I Interferon Responses in Human Monocyte-Derived Cells
    Authors: J Paijo, M Döring, J Spanier, E Grabski, M Nooruzzama, T Schmidt, G Witte, M Messerle, V Hornung, V Kaever, U Kalinke
    PLoS Pathog, 2016;12(4):e1005546.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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