Human sTNF RI/TNFRSF1A Quantikine ELISA Kit

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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (200 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Citrate Plasma (20 uL), Urine (20 uL)
  • Sensitivity
    1.2 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
  • Specificity
    Natural and recombinant human sTNF RI
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC05, Quantikine Immunoassay Control Group 2 - Please Inquire
Product Summary
The Quantikine Human sTNF RI Immunoassay is a 4.5 hour solid phase ELISA designed to measure sTNF RI in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human sTNF RI, as well as antibodies raised against this polypeptide. The recombinant protein represents the non-glycosylated, N-terminal methionyl form of the naturally occurring human soluble Type I receptor for TNF with an apparent molecular weight of approximately 18.6 kDa. This immunoassay has been shown to accurately quantitate the recombinant sTNF RI. Results obtained on samples containing natural sTNF RI showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural sTNF RI. Since the measurement of human sTNF RI by this immunoassay is relatively insensitive to added TNF-alpha or TNF-beta, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples (i.e. the total amount of free receptor plus the total amount of receptor bound to TNF).

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation3.247.1717.84.89.320.6

Cell Culture Supernates, Urine
Intra-Assay Precision Inter-Assay Precision
Standard Deviation2.145.38112.79.723.3


The recovery of sTNF RI spiked to three levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 85 80-92
EDTA Plasma (n=8) 86 70-97
Heparin Plasma (n=8) 93 79-103
Serum (n=8) 90 77-103
Urine (n=8) 85 71-109
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of sTNF RI were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human sTNF RI/TNFRSF1A Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TNF RI/TNFRSF1A
TNF RI (TNF receptor 1; TNF R-p55/p60) is a transmembrane receptor for TNF-alpha and Lymphotoxin-alpha/TNF-beta. A soluble decoy form of the receptor is shed by ADAM-17/TACE in response to inflammatory stimulation. TNF RI signaling through NFkB is essential for the development of lymph node germinal centers and Peyer’s patches and for combating intracellular pathogens such as Listeria.
    • Long Name
      Tumor Necrosis Factor Receptor I
    • Entrez Gene IDs
      7132 (Human); 21937 (Mouse); 25625 (Rat);
    • Alternate Names
      CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 200 µL Standard, Control, or Sample
    6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 3 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 38
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    Sample Type
    1. Local TNF causes NFATc1-dependent cholesterol-mediated podocyte injury
      J Clin Invest, 2016;0(0):.
      Species: Human
      Sample Type: Serum
    2. T. vaginalis Infection Is Associated with Increased IL-8 and TNFr1 Levels but with the Absence of CD38 and HLADR Activation in the Cervix of ESN.
      Authors: Jarrett O, Brady K, Modur S, Plants J, Landay A, Ghassemi M, Golub E, Spear G, Novak R
      PLoS ONE, 2015;10(6):e0130146.
      Species: Human
      Sample Type: Cervical Fluid
    3. Circulating TNF receptors 1 and 2 are associated with the severity of renal interstitial fibrosis in IgA nephropathy.
      Authors: Sonoda Y, Gohda T, Suzuki Y, Omote K, Ishizaka M, Matsuoka J, Tomino Y
      PLoS ONE, 2015;10(4):e0122212.
      Species: Human
      Sample Type: Serum
    4. Plasma TNF-alpha and Soluble TNF Receptor Levels after Doxorubicin with or without Co-Administration of Mesna-A Randomized, Cross-Over Clinical Study.
      Authors: Hayslip J, Dressler E, Weiss H, Taylor T, Chambers M, Noel T, Miriyala S, Keeney J, Ren X, Sultana R, Vore M, Butterfield D, St Clair D, Moscow J
      PLoS ONE, 2015;10(4):e0124988.
      Species: Human
      Sample Type: Plasma
    5. Late Antiretroviral Therapy (ART) Initiation Is Associated with Long-Term Persistence of Systemic Inflammation and Metabolic Abnormalities.
      Authors: Ghislain M, Bastard J, Meyer L, Capeau J, Fellahi S, Gerard L, May T, Simon A, Vigouroux C, Goujard C
      PLoS ONE, 2015;10(12):e0144317.
      Species: Human
      Sample Type: Serum
    6. A case-control study of pre-operative levels of serum neutrophil gelatinase-associated lipocalin and other potential inflammatory markers in colorectal cancer.
      Authors: Duvillard L, Ortega-Deballon P, Bourredjem A, Scherrer M, Mantion G, Delhorme J, Deguelte-Lardiere S, Petit J, Bonithon-Kopp C
      BMC Cancer, 2014;14(0):912.
      Species: Human
      Sample Type: Serum
    7. Dynamics of immune reconstitution and activation markers in HIV+ treatment-naive patients treated with raltegravir, tenofovir disoproxil fumarate and emtricitabine.
      Authors: Funderburg N, Andrade A, Chan E, Rosenkranz S, Lu D, Clagett B, Pilch-Cooper H, Rodriguez B, Feinberg J, Daar E, Mellors J, Kuritzkes D, Jacobson J, Lederman M
      PLoS ONE, 2013;8(12):e83514.
      Species: Human
      Sample Type: Plasma
    8. Inflammation marker and risk of pancreatic cancer: a nested case-control study within the EPIC cohort.
      Authors: Grote VA, Kaaks R, Nieters A, Tjonneland A, Halkjaer J, Overvad K, Skjelbo Nielsen MR, Boutron-Ruault MC, Clavel-Chapelon F, Racine A, Teucher B, Becker S, Pischon T, Boeing H, Trichopoulou A, Cassapa C, Stratigakou V, Palli D, Krogh V, Tumino R, Vineis P, Panico S, Rodriguez L, Duell EJ, Sanchez MJ, Dorronsoro M, Navarro C, Gurrea AB, Siersema PD, Peeters PH, Ye W, Sund M, Lindkvist B, Johansen D, Khaw KT, Wareham N, Allen NE, Travis RC, Fedirko V, Jenab M, Michaud DS, Chuang SC, Romaguera D, Bueno-de-Mesquita HB, Rohrmann S
      Br. J. Cancer, 2012;106(11):1866-74.
      Species: Human
      Sample Type: Serum
    9. Inflammatory markers and the risk of hip fracture: the Women's Health Initiative.
      Authors: Barbour KE, Boudreau R, Danielson ME, Youk AO, Wactawski-Wende J, Greep NC, LaCroix AZ, Jackson RD, Wallace RB, Bauer DC, Allison MA, Cauley JA
      J. Bone Miner. Res., 2012;27(5):1167-76.
      Species: Human
      Sample Type: Serum
    10. Compensated inflammation in systemic juvenile idiopathic arthritis: role of alternatively activated macrophages.
      Authors: Shimizu M, Yachie A
      Cytokine, 2012;60(1):226-32.
      Species: Human
      Sample Type: Serum
    11. Cytokine Response Patterns in Severe Pandemic 2009 H1N1 and Seasonal Influenza among Hospitalized Adults.
      Authors: Lee N, Wong CK, Chan PK, Chan MC, Wong RY, Lun SW, Ngai KL, Lui GC, Wong BC, Lee SK, Choi KW, Hui DS
      PLoS ONE, 2011;6(10):e26050.
      Species: Human
      Sample Type: Plasma
    12. Elevated CSF levels of TACE activity and soluble TNF receptors in subjects with mild cognitive impairment and patients with Alzheimer's disease.
      Authors: Jiang H, Hampel H, Prvulovic D, Wallin A, Blennow K, Li R, Shen Y
      Mol Neurodegener, 2011;6(0):69.
      Species: Human
      Sample Type: CSF
    13. Matrix metalloproteinase-9 and plasminogen activator inhibitor-1 are associated with right ventricular structure and function: the MESA-RV Study.
      Authors: Kawut SM, Barr RG, Johnson WC
      Biomarkers, 2010;15(8):731-8.
      Species: Human
      Sample Type: Plasma
    14. Adrenomedullin and endothelin-1 are related to inflammation in chronic heart failure.
      Authors: Gombos T, Forhecz Z, Pozsonyi Z, Wallentin S, Papassotiriou J, Kunde J, Morgenthaler NG, Janoskuti L, Prohaszka Z
      Inflamm. Res., 2009;58(6):298-305.
      Species: Human
      Sample Type: Serum
    15. Comparing Type D personality and older age as correlates of tumor necrosis factor-alpha dysregulation in chronic heart failure.
      Authors: Denollet J, Vrints CJ, Conraads VM
      Brain Behav. Immun., 2008;22(5):736-43.
      Species: Human
      Sample Type: Plasma
    16. Change in plasma tumor necrosis factor receptor 1 levels in the first week after myeloablative allogeneic transplantation correlates with severity and incidence of GVHD and survival.
      Authors: Choi SW, Kitko CL, Braun T, Paczesny S, Yanik G, Mineishi S, Krijanovski O, Jones D, Whitfield J, Cooke K, Hutchinson RJ, Ferrara JL, Levine JE
      Blood, 2008;112(4):1539-42.
      Species: Human
      Sample Type: Plasma
    17. Selective effects of CPAP on sleep apnoea-associated manifestations.
      Authors: Vgontzas AN, Zoumakis E, Bixler EO, Lin HM, Collins B, Basta M, Pejovic S, Chrousos GP
      Eur. J. Clin. Invest., 2008;38(8):585-95.
      Species: Human
      Sample Type: Plasma
    18. Microparticles of human atherosclerotic plaques enhance the shedding of the tumor necrosis factor-alpha converting enzyme/ADAM17 substrates, tumor necrosis factor and tumor necrosis factor receptor-1.
      Authors: Canault M, Leroyer AS, Peiretti F, Leseche G, Tedgui A, Bonardo B, Alessi MC, Boulanger CM, Nalbone G
      Am. J. Pathol., 2007;171(5):1713-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Selective functional exhaustion of hematopoietic progenitor cells in the bone marrow of patients with postinfarction heart failure.
      Authors: Kissel CK, Lehmann R, Assmus B, Aicher A, Honold J, Fischer-Rasokat U, Heeschen C, Spyridopoulos I, Dimmeler S, Zeiher AM
      J. Am. Coll. Cardiol., 2007;49(24):2341-9.
      Species: Human
      Sample Type: Serum
    20. Inflammatory markers and incident fracture risk in older men and women: the Health Aging and Body Composition Study.
      Authors: Cauley JA, Danielson ME, Boudreau RM, Forrest KY, Zmuda JM, Pahor M, Tylavsky FA, Cummings SR, Harris TB, Newman AB
      J. Bone Miner. Res., 2007;22(7):1088-95.
      Species: Human
      Sample Type: Serum
    21. Serum interleukin-18 and soluble tumour necrosis factor receptor 2 are associated with disease severity in patients with paracoccidioidomycosis.
      Authors: Corvino CL, Mamoni RL, Fagundes GZ, Blotta MH
      Clin. Exp. Immunol., 2007;147(3):483-90.
      Species: Human
      Sample Type: Serum
    22. Muscle atrophy and hypertrophy signaling in patients with chronic obstructive pulmonary disease.
      Authors: Doucet M, Russell AP, Leger B, Debigare R, Joanisse DR, Caron MA, LeBlanc P, Maltais F
      Am. J. Respir. Crit. Care Med., 2007;176(3):261-9.
      Species: Human
      Sample Type: Plasma
    23. Neutrophils from patients with TNFRSF1A mutations display resistance to tumor necrosis factor-induced apoptosis: pathogenetic and clinical implications.
      Authors: D&amp;amp;apos;Osualdo A
      Arthritis Rheum., 2006;54(3):998-1008.
      Species: Human
      Sample Type: Cell Culture Supernates
    24. Proinflammatory cytokines, insulin resistance, and insulin secretion in chronic hepatitis C patients: A case-control study.
      Authors: Lecube A, Hernandez C, Genesca J, Simo R
      Diabetes Care, 2006;29(5):1096-101.
      Species: Human
      Sample Type: Serum
    25. Experimental heatstroke in baboon: analysis of the systemic inflammatory response.
      Authors: Bouchama A, Ollivier V, Roberts G, Al Mohanna F, de Prost D, Eldali A, Saussereau E, El-Sayed R, Chollet-Martin S
      Shock, 2005;24(4):332-5.
      Species: Primate – Papio hamadryas (Hamadyras Baboon)
      Sample Type: Plasma
    26. Growth hormone-induced reduction of soluble apoptosis mediators is associated with reverse cardiac remodelling and improvement of exercise capacity in patients with idiopathic dilated cardiomyopathy.
      Authors: Parissis JT, Adamopoulos S, Karatzas D, Paraskevaidis J, Livanis E, Kremastinos D
      Eur J Cardiovasc Prev Rehabil, 2005;12(2):164-8.
      Species: Human
      Sample Type: Plasma
    27. Analysis of tumor necrosis factor-alpha, interleukin-6, interleukin-1beta, soluble tumor necrosis factor receptors I and II, interleukin-6 soluble receptor, interleukin-1 soluble receptor type II, interleukin-1 receptor antagonist, and protein in the synovial fluid of patients with temporomandibular joint disorders.
      Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
      Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005;99(3):276-84.
      Species: Human
      Sample Type: Synovial Fluid
    28. Levels of soluble cytokine factors in temporomandibular joint effusions seen on magnetic resonance images.
      Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
      Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005;99(4):411-8.
      Species: Human
      Sample Type: Synovial Fluid
    29. Regulation of peritoneal and systemic neutrophil-derived tumor necrosis factor-alpha release in patients with severe peritonitis: role of tumor necrosis factor-alpha converting enzyme cleavage.
      Authors: Kermarrec N, Selloum S, Plantefeve G, Chosidow D, Paoletti X, Lopez A, Mantz J, Desmonts JM, Gougerot-Pocidalo MA, Chollet-Martin S
      Crit. Care Med., 2005;33(6):1359-64.
      Species: Human
      Sample Type: Cell Culture Supernates
    30. Effects of the active metabolite of leflunomide, A77 1726, on cytokine release and the MAPK signalling pathway in human rheumatoid arthritis synoviocytes.
      Authors: Vergne-Salle&lt;/LastName&gt;&lt;F P&lt;/Initial, Vergne-Salle P, Leger DY, Bertin P, Treves R, Beneytout JL, Liagre B
      Cytokine, 2005;31(5):335-48.
      Species: Human
      Sample Type: Cell Culture Supernates
    31. Pro- and anti-inflammatory responses in respiratory syncytial virus bronchiolitis.
      Authors: McNamara PS, Flanagan BF, Selby AM, Hart CA, Smyth RL
      Eur. Respir. J., 2004;23(1):106-12.
      Species: Human
      Sample Type: BALF
    32. Gene transfer of tumor necrosis factor inhibitor improves the function of lung allografts.
      Authors: Tagawa T, Kozower BD, Kanaan SA, Daddi N, Muraoka M, Oka T, Ritter JH, Patterson GA
      J. Thorac. Cardiovasc. Surg., 2004;127(6):1558-63.
      Species: Human
      Sample Type: Tissue Homogenates
    33. Release of full-length 55-kDa TNF receptor 1 in exosome-like vesicles: a mechanism for generation of soluble cytokine receptors.
      Authors: Hawari FI, Rouhani FN, Cui X, Yu ZX, Buckley C, Kaler M, Levine SJ
      Proc. Natl. Acad. Sci. U.S.A., 2004;101(5):1297-302.
      Species: Human
      Sample Type: Cell Culture Supernates
    34. Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
      Authors: Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
      J. Virol., 2003;77(3):1727-37.
      Species: Primate - Macaca mulatta (Rhesus monkey)
      Sample Type: Serum
    35. Age-related autoantibody production in a nonhuman primate model.
      Authors: Attanasio R, Brasky KM, Robbins SH, Jayashankar L, Nash RJ, Butler TM
      Clin. Exp. Immunol., 2001;123(3):361-5.
      Species: Primate-Papio cynocephalus anubis (baboon)
      Sample Type: Serum
    36. Tumour necrosis factor alpha and its soluble receptors in juvenile chronic arthritis.
      Authors: Rooney M, Varsani H, Martin K, Lombard PR, Dayer JM, Woo P
      Rheumatology (Oxford), 2000;39(4):432-8.
      Species: Human
      Sample Type: Serum
    37. Proinflammatory cytokines increase in sepsis after anti-adhesion molecule therapy.
      Authors: Welty-Wolf KE, Carraway MS, Ghio A, Kantrow SP, Huang YC, Piantadosi CA
      Shock, 2000;13(5):404-9.
      Species: Primate – Papio cynocephalus (Baboon)
      Sample Type: Serum
    38. An analysis of 14 molecular markers for monitoring osteoarthritis: segregation of the markers into clusters and distinguishing osteoarthritis at baseline.
      Authors: Otterness IG, Swindell AC, Zimmerer RO, Poole AR, Ionescu M, Weiner E
      Osteoarthr. Cartil., 2000;8(3):180-5.
      Species: Human
      Sample Type: Serum
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