Human TNF RI/TNFRSF1A Quantikine QuicKit ELISA

Catalog # Availability Size / Price Qty
QK225
Control Products Available
Human TNF RI ELISA Standard Curve
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Product Details
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Human TNF RI/TNFRSF1A Quantikine QuicKit ELISA Summary

Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL), Urine (13 uL)
Sensitivity
1.69 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human TNF RI.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® QuicKit™ Human TNF RI/TNFRSF1A Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human TNF RI levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human TNF RI and antibodies raised against the recombinant protein. Results obtained for natural human TNF RI showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit™ standards. These results indicate that this kit can be used to determine relative mass values for natural human TNF RI.

Precision

Intra-Assay Precision (Precision within an assay) <div>Two samples of known concentration were tested twenty times on one plate to assess&nbsp;intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 1
n 20 20 10 10
Mean 100 624 619 99.9
Standard Deviation 1.75 17 39.3 6.78
CV% 1.8 2.7 6.3 6.8

Recovery

The recovery of human TNF RI spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 92-109
EDTA Plasma (n=2) 98 88-109
Heparin Plasma (n=2) 98 85-109
Serum (n=2) 99 91-113
Urine (n=2) 96 92-100

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human TNF RI were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human TNF RI ELISA Linearity

Data Examples

Human TNF RI ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TNF RI/TNFRSF1A

TNF RI (TNF receptor 1; TNF R-p55/p60) is a transmembrane receptor for TNF-alpha and Lymphotoxin-alpha/TNF-beta. A soluble decoy form of the receptor is shed by ADAM-17/TACE in response to inflammatory stimulation. TNF RI signaling through NFkB is essential for the development of lymph node germinal centers and Peyer’s patches and for combating intracellular pathogens such as Listeria.

Long Name:
Tumor Necrosis Factor Receptor I
Entrez Gene IDs:
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names:
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

FAQs

No product specific FAQs exist for this product, however you may

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