Human TNF RI/TNFRSF1A Quantikine QuicKit ELISA Summary
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL), Urine (13 uL)
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Natural and recombinant human TNF RI.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine® QuicKit™ Human TNF RI/TNFRSF1A Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human TNF RI levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human TNF RI and antibodies raised against the recombinant protein. Results obtained for natural human TNF RI showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit™ standards. These results indicate that this kit can be used to determine relative mass values for natural human TNF RI.
Intra-Assay Precision (Precision within an assay) <div>Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
The recovery of human TNF RI spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
||Average % Recovery
|Cell Culture Media (n=4)
|EDTA Plasma (n=2)
|Heparin Plasma (n=2)
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human TNF RI were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human TNF RI ELISA Standard Curve
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TNF RI/TNFRSF1A
TNF RI (TNF receptor 1; TNF R-p55/p60) is a transmembrane receptor for TNF-alpha and Lymphotoxin-alpha/TNF-beta. A soluble decoy form of the receptor is shed by ADAM-17/TACE in response to inflammatory stimulation. TNF RI signaling through NFkB is essential for the development of lymph node germinal centers and Peyer’s patches and for combating intracellular pathogens such as Listeria.
Tumor Necrosis Factor Receptor I
Entrez Gene IDs:
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor