Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 401513
View all SUMO3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SUMO3
Met1-Phe103
Accession # P55854

Specificity

Detects human SUMO3 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human SUMO3 Antibody

SUMO3 antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC).

SUMO3 in HEK293 Human Cell Line.

SUMO3 was detected in immersion fixed HEK293 human embryonic kidney cell line using Rat Anti-Human SUMO3 Monoclonal Antibody (Catalog # MAB2959) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human SUMO3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Reviewed Applications

Read 1 review rated 4 using MAB2959 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SUMO3

Small Ubiquitin-like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation. Unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All SUMO proteins share the conserved ubiquitin domain and the C‑terminal diglycine cleavage/attachment site. Human SUMO3, also known as SMT3A, is synthesized as a 103 amino acid (aa), 11.5 kDa propeptide that contains an 11 aa C‑terminal prosegment. Following prosegment cleavage, the C‑terminal glycine is enzymatically attached to a lysine on a target protein. Human SUMO3 shares 83% sequence identity with SUMO3 from mouse. SUMO3 also has very high sequence homology to SUMO2 and SUMO4, 87 % and 75%, respectively. SUMO3 shares only 47% sequence identity to SUMO1.

Long Name

Small Ubiquitin-like Modifier 3

Alternate Names

SMT3A, SMT3H1

Entrez Gene IDs

6612 (Human)

Gene Symbol

SUMO3

UniProt

P55854

Additional SUMO3 Products

Product Documents for Human SUMO3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SUMO3 Antibody

For research use only

Customer Reviews for Human SUMO3 Antibody (1)

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  • Human SUMO3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: recombinant human protein
    Species: Human
    Verified Customer | Posted 03/31/2021
    Western blot: left to right: sumo3 conjugated-protein,5ng,10ng,50ng,Sumo3 protein(unconjugated) 5ng,10ng,50ng, protein size marker
    1/1000 primary antibody dilution
    Human SUMO3 Antibody MAB2959

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