Detection of Human Syntaxin 12 by Western Blot.
Western blot shows lysates of CCD‑1070Sk human foreskin fibroblast cell line, LNCaP human prostate cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human Syntaxin 12 Monoclonal Antibody (Catalog # MAB6617) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Syntaxin 12 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Syntaxin 12
Syntaxin 12/STX12 is a 39 kDa member of the Syntaxin family. It is a SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network. Human STX12 is 276 amino acids (aa) in length, and is a type IV single-pass transmembrane protein. The cytoplasmic region extends from aa 1-248. A coiled-coil region extends from aa 33-131 within that region. From aa 178-240, there is a t-SNARE coiled-coil homology domain. The transmembrane region is located at 249-269, and a vesicular area is located at aa 270-276. Human STX12 shares 92% aa sequence identity with mouse STX12.
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