Human T-bet/TBX21 Alexa Fluor® 488-conjugated Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human T‑bet/TBX21 in direct ELISAs.
  • Source
    Recombinant Monoclonal Mouse IgG1 Clone # 525831
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    E. coli-derived recombinant human T‑bet/TBX21
    Glu326-Asn535
    Accession # Q9UL17
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of T‑bet/TBX21 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD8 alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB1509A) and either (A) Mouse Anti-Human T‑bet/TBX21 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC53851G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # IC002G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of T‑bet/TBX21 in CD4 + Human PBMCs Stimulated to Induce Th1 Cells by Flow Cytometry. CD4+ Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA) and 10 ng/mL Recombinant Human IL‑12 (Catalog # 219-IL) for 5 days to induce Th1 cell development for were stained with Mouse Anti-Human IFN‑ gamma PE‑conjugated Monoclonal Antibody (Catalog # IC285P) and either (A) Mouse Anti-Human T‑bet/TBX21 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC53851G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # IC002G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: T-bet/TBX21

T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1 cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN-gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.

  • Long Name:
    T-box transcription factor TBX21
  • Entrez Gene IDs:
    30009 (Human); 57765 (Mouse); 303496 (Rat)
  • Alternate Names:
    Tbet; T-bet; TBETT-PET; TBLYM; TBLYMT-cell-specific T-box transcription factor T-bet; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; TBX21; T-PET; Transcription factor TBLYM
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

Ctrl IC002G 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human T-bet/TBX21 Alexa Fluor® 488-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Associated Pathways

Customers Who Viewed
This Item Also Viewed

Feedback

Customer Information