Human T-bet/TBX21 Alexa Fluor® 488-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC53851G-025
Detection of T‑bet/TBX21 in Human PBMCs by Flow Cytometry.
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Human T-bet/TBX21 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human T‑bet/TBX21 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 525831
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human T‑bet/TBX21
Glu326-Asn535
Accession # Q9UL17
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of T‑bet/TBX21 in Human PBMCs by Flow Cytometry. View Larger

Detection of T‑bet/TBX21 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD8 alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB1509A) and either (A) Mouse Anti-Human T‑bet/TBX21 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC53851G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # IC002G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of T‑bet/TBX21 in CD4 + Human PBMCs Stimulated to Induce Th1 Cells by Flow Cytometry. View Larger

Detection of T‑bet/TBX21 in CD4 + Human PBMCs Stimulated to Induce Th1 Cells by Flow Cytometry. CD4+ Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA) and 10 ng/mL Recombinant Human IL‑12 (Catalog # 219-IL) for 5 days to induce Th1 cell development for were stained with Mouse Anti-Human IFN‑ gamma PE‑conjugated Monoclonal Antibody (Catalog # IC285P) and either (A) Mouse Anti-Human T‑bet/TBX21 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC53851G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # IC002G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: T-bet/TBX21

T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1 cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN-gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.

Long Name
T-box transcription factor TBX21
Entrez Gene IDs
30009 (Human); 57765 (Mouse); 303496 (Rat)
Alternate Names
Tbet; T-bet; TBETT-PET; TBLYM; TBLYMT-cell-specific T-box transcription factor T-bet; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; TBX21; T-PET; Transcription factor TBLYM

Product Datasheets

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