Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 525831
View all T-bet/TBX21 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human T‑bet/TBX21
Glu326-Asn535
Accession # Q9UL17

Specificity

Detects human T‑bet/TBX21 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human T-bet/TBX21 MAb (Clone 525831)

Detection of T‑bet/TBX21 antibody in Human PBMCs Stimulated to Induce Th1 Cells antibody by Flow Cytometry.

Detection of T‑bet/TBX21 in Human PBMCs Stimulated to Induce Th1 Cells by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA; 20 µg/mL) and 10 ng/mL Recombinant Human IL-12 (Catalog # 219-IL) for 5 days to induce Th1 cell development were stained with Mouse Anti-Human T-bet/TBX21 Monoclonal Antibody (Catalog # MAB53851) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # MAB002). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Applications for Human T-bet/TBX21 MAb (Clone 525831)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA; 20 μg/mL) and Recombinant Human IL‑12 (Catalog # 219-IL;10 ng/mL) for 5 days to induce Th1 cell development were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Reviewed Applications

Read 1 review rated 5 using MAB53851 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: T-bet/TBX21

T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1 cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN-gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.

Long Name

T-box transcription factor TBX21

Alternate Names

T-PET, Tbet, TBLYM, TBX21

Entrez Gene IDs

30009 (Human); 57765 (Mouse); 303496 (Rat)

Gene Symbol

TBX21

UniProt

Q9UL17 (Human)

Additional T-bet/TBX21 Products

Product Documents for Human T-bet/TBX21 MAb (Clone 525831)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human T-bet/TBX21 MAb (Clone 525831)

For research use only

Customer Reviews for Human T-bet/TBX21 MAb (Clone 525831) (1)

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Customer Images

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  • Human T-bet/TBX21 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Tonsil
    Species: Human
    Verified Customer | Posted 03/30/2020
    Tbet 1:100 on tonsil
    Human T-bet/TBX21 MAb (Clone 525831) MAB53851

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