Human TBX2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human TBX2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TBX3, rhTBX6, or rhT‑bet is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 393720
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human TBX2
    Thr593-Arg702
    Accession # Q13207
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
TBX2 in MCF‑7 Human Cell Line. TBX2 was detected in immersion fixed MCF‑7 human breast cancer cell line using Rat Anti-Human TBX2 Monoclonal Antibody (Catalog # MAB50401) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TBX2

T-box protein 2 (TBX2) is a member of the T-box family of transcription factors and functions in the transcriptional regulation of genes required for mesoderm differentiation and limb bud pattern formation. The 74 kDa (predicted MW) nuclear protein, like other T-box family members, contains a T-domain which is involved in DNA binding and dimerization of the protein (aa 99‑277). Human TBX2 shows 96%, 95%, and 71% amino acid identity with dog, mouse, and Xenopus tropicalis TBX2, respectively.

  • Long Name:
    T-box Transcription Factor 2
  • Entrez Gene IDs:
    6909 (Human); 21385 (Mouse); 303398 (Rat)
  • Alternate Names:
    FLJ10169; T-box 2; T-box protein 2; T-box transcription factor TBX2; TBX2
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