Human TDRD1 Antibody

Catalog # Availability Size / Price Qty
AF6296
AF6296-SP
Human TDRD1 Antibody in Western Blot
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Human TDRD1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TDRD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse TDRD1 is observed and less than 1% cross-reactivity with recombinant human TDRKH is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TDRD1
Asn837-Lys968
Accession # Q9BXT4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human TDRD1 by Western Blot. View Larger

Detection of Human TDRD1 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TDRD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6296) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for TDRD1 at approximately 75 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TDRD1

TDRD1 (Tudor domain containing protein 1; also CT41.1) is a 120-140 kDa member of the Tudor family of proteins. It is expressed in spermatocytes and spermatids, and forms part of an RNA-silencing complex that regulates gene expression. TDRD1, through its Tudor and MYND domains, binds methylated PIWI (P-element-induced wimpy testis) protein and PIWI-interacting RNA loading factor, respectively. This serves to promote the recruitment of germline-specific small RNAs into a PIWI ribonucleoprotein complex. Human TDRD1 is 1180 aa in length. It contains one MYND-type Zn finger region (aa 170-206), and four methyl-binding Tudor domains (aa 312-1048). There are four potential splice variants with predicted molecular weights ranging between 79 kDa and 133 kDa. One contains an alternate start site at Met404, another contains an alternative start site at Met22, accompanied by deletions of aa 554-610 and 775-831, a third shows a 10 aa substitution for Ser1180, while a fourth contains deletions of aa 328-375, 554-610 and 1059-1134. Over aa 837-968, human TDRD1 shares 66% aa identity with mouse TDRD1.

Long Name
Tudor Domain Containing 1
Entrez Gene IDs
56165 (Human); 83561 (Mouse); 292129 (Rat)
Alternate Names
CT41.1; CT41.1Cancer/testis antigen 41.1; FLJ21082; TDRD1; tudor domain containing 1; tudor domain containing protein 1; tudor domain-containing protein 1

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