Human TEM7/PLXDC1 Isoform 1 Antibody Summary
Accession # ACE86780
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human TEM7/PLXDC1 Isoform 1 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line cancer tissue, HeLa human cervical epithelial carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TEM7/PLXDC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5327) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TEM7 Isoform 1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
TEM7 (Tumor endothelial marker 7; also TEM3 and Plexin domain-containing protein-1/PXDC1) is an 85 kDa member of the plexin family of proteins. It is one of a number of TEM designated molecules that are related only a name and not structural similarity. TEM7 is found on tumor endothelial cells, newly formed endothelial cells and neurons. It is known to bind nidogen. Mature human TEM7 is a type I transmembrane glycoprotein 482 amino acids (aa) in length. It contains an extracellular region (aa 19‑426) with one plexin domain (aa 303‑347). There are three isoform variants. One is intracellular and shows an alternate start site at Met74 accompanied by a 28 aa substitution for aa 331‑500. The other two are soluble, with one showing only the above 28 aa substitution and a second showing an eight aa substitution for aa 331‑500. Over aa 19‑426, human TEM7 shares 81% aa identity with mouse TEM7.
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