Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all TEM7/PLXDC1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TEM7/PLXDC1 Isoform 1
Leu19-Thr426
Accession # ACE86780

Specificity

Detects human TEM7/PLXDC1 Isoform 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TEM8 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human TEM7/PLXDC1 Isoform 1 Antibody

Detection of Human TEM7/PLXDC1 Isoform 1 antibody by Western Blot.

Detection of Human TEM7/PLXDC1 Isoform 1 by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line cancer tissue, HeLa human cervical epithelial carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TEM7/PLXDC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5327) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TEM7 Isoform 1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human TEM7/PLXDC1 Isoform 1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line cancer tissue, HeLa human cervical epithelial carcinoma cell line, and MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TEM7/PLXDC1

TEM7 (Tumor endothelial marker 7; also TEM3 and Plexin domain-containing protein-1/PXDC1) is an 85 kDa member of the plexin family of proteins. It is one of a number of TEM designated molecules that are related only a name and not structural similarity. TEM7 is found on tumor endothelial cells, newly formed endothelial cells and neurons. It is known to bind nidogen. Mature human TEM7 is a type I transmembrane glycoprotein 482 amino acids (aa) in length. It contains an extracellular region (aa 19‑426) with one plexin domain (aa 303‑347). There are three isoform variants. One is intracellular and shows an alternate start site at Met74 accompanied by a 28 aa substitution for aa 331‑500. The other two are soluble, with one showing only the above 28 aa substitution and a second showing an eight aa substitution for aa 331‑500. Over aa 19‑426, human TEM7 shares 81% aa identity with mouse TEM7.

Long Name

Tumor Endothelial Marker 7/Plexin Domain Containing Protein 1

Alternate Names

PLXDC1, TEM3

Entrez Gene IDs

57125 (Human); 72324 (Mouse); 303505 (Rat)

Gene Symbol

PLXDC1

UniProt

ACE86780

Additional TEM7/PLXDC1 Products

Product Documents for Human TEM7/PLXDC1 Isoform 1 Antibody

Certificate of Analysis

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Product Specific Notices for Human TEM7/PLXDC1 Isoform 1 Antibody

For research use only

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