|Detection of Human Teneurin‑1 by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human Teneurin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6324) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Teneurin‑1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
Teneurin-1 (also Ten-m1, tenascin-M1, and Ten-m/Odz1) is a 250-300 kDa member of the tenascin family, teneurin subfamily of transmembrane (TM) molecules. It is a covalently-linked homodimer that is expressed in both embryonic and adult neurons, among which are cerebellar granule cells and CA2 pyramidal hippocampal neurons. Teneurin 1 appears to promote neurite outgrowth and mediate cell-to-cell adhesion via homophilic interactions. Human Teneurin-1 is a 2725 amino acid (aa) type II TM glycoprotein. It contains a 324 aa cytoplasmic region (aa 1-324) that contains an NLS (aa 62‑65), plus a 2380 aa extracellular domain (ECD). The ECD possesses eight sequential EGF-like domains (aa 528-796), five NHL repeats, each of which form a beta -propeller (aa 1194-1524), and 23 YD/TyrAsp-containing repeats that bind carbohydrates. Cleavage at the N-terminus generates an initial 65 kDa membrane spanning fragment, followed by TM cleavage that generates a 45 kDa cytosolic fragment. C-terminal cleavage generates a short 5 kDa, 41 aa peptide (aa 2682-2722) termed TCAP-1 that shows bioactivity. One splice variant shows a deletion of aa 1232-1239. Over aa 1-317, human Teneurin-1 shares 96% aa identity with mouse Teneurin-1.
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