Human Teneurin-1 Antibody

Detection of Human Teneurin‑1 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human Teneurin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6324) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Teneurin-1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Teneurin-1 by Western Blot RNA interference by p38-specific siRNAs neutralizes the EGF-promoted upregulation of ODZ1. (A) GBM cells were transfected with two MAPK14-specific siRNAs or two control siRNAs. Following incubation with EGF, the protein levels of ODZ1 were determined by Western Blot analysis. (B) Same experimental design as in A but uses MAPK11-specific siRNAs. Additionally, alpha Tubulin was used to ensure equal loading. (C) Quantification of ODZ1 protein levels of the experiment shown in B by using imageJ software (v1.53k). (D) Cells carrying siRNAs specific to MAPK11 were analyzed for the expression of either MAPK11 or MAPK14 mRNA by qPCR. Histograms represent the mean of three independent experiments ± S.D. ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38727302), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Teneurin-1 by Western Blot The blockade of the EGFR–p38 signaling downregulates the levels of ODZ1. (A) GBM cells were treated with EGF in the absence or in the presence of the EGFR-blocking antibody Cetuximab or the specific p38 MAPK inhibitor SB203580. Western Blot analysis confirmed that the blockade of EGFR/p38 inhibited the phosphorylation of MAPKAPK2 at Thr222, a target of p38, and reduced the expression of the ODZ1 protein. (B) Downregulation of ODZ1 following inhibition of EGFR/p38 was confirmed in two additional GBM cell lines. MAPKAPK2 and alpha Tubulin were used to ensure equal loading. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38727302), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Teneurin-1 by Western Blot The blockade of the EGFR–p38 signaling downregulates the levels of ODZ1. (A) GBM cells were treated with EGF in the absence or in the presence of the EGFR-blocking antibody Cetuximab or the specific p38 MAPK inhibitor SB203580. Western Blot analysis confirmed that the blockade of EGFR/p38 inhibited the phosphorylation of MAPKAPK2 at Thr222, a target of p38, and reduced the expression of the ODZ1 protein. (B) Downregulation of ODZ1 following inhibition of EGFR/p38 was confirmed in two additional GBM cell lines. MAPKAPK2 and alpha Tubulin were used to ensure equal loading. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38727302), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Teneurin-1 by Western Blot RNA interference by p38-specific siRNAs neutralizes the EGF-promoted upregulation of ODZ1. (A) GBM cells were transfected with two MAPK14-specific siRNAs or two control siRNAs. Following incubation with EGF, the protein levels of ODZ1 were determined by Western Blot analysis. (B) Same experimental design as in A but uses MAPK11-specific siRNAs. Additionally, alpha Tubulin was used to ensure equal loading. (C) Quantification of ODZ1 protein levels of the experiment shown in B by using imageJ software (v1.53k). (D) Cells carrying siRNAs specific to MAPK11 were analyzed for the expression of either MAPK11 or MAPK14 mRNA by qPCR. Histograms represent the mean of three independent experiments ± S.D. ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38727302), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Teneurin-1 by Western Blot EGF induces the expression of ODZ1. (A) FACS analysis of two GBM cell lines expressing, or not, EGFR (same as in Figure 2A). (B) GBM cells were incubated with EGF and the mRNA levels of ODZ1 were determined after 24 h by qPCR. Histograms represent the mean of three independent experiments ± S.D. Asterisks represent significant differences (** p < 0.01). (C) Western Blot analysis confirmed that the exposure of GBM cells to EGF also promoted the expression of ODZ1 at the protein level. Additionally, alpha Tubulin was used to ensure equal loading. Arrows indicate the native high-molecular-weight ODZ1 protein and its most frequent proteolytic fragment of 70 kDa. (D) Cells were incubated in the presence of EGF and migration was determined at different time points by using an in vitro wound healing assay. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38727302), licensed under a CC-BY license. Not internally tested by R&D Systems.