Human TFF2 DuoSet ELISA

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Human TFF2 ELISA Standard Curve
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Product Details
Citations (3)
Supplemental Products

Human TFF2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human TFF2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human TFF2 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TFF2

The trefoil factor (TFF) family contains three secreted, protease-resistant polypeptides that help to maintain gastrointestinal system integrity. Family members are 7-12 kDa in size, and contain a 3-disulfide bond generated trefoil structure that resembles a meadow clover. TFF1, 2 and 3 are produced in different areas of the digestive tract, and promote restitution, or the resealing of superficial wounds after injury.

Long Name:
Trefoil Factor 2
Entrez Gene IDs:
7032 (Human); 21785 (Mouse); 116592 (Rat)
Alternate Names:
SML1; SML1trefoil factor 2, SML1, human spasmolytic polypeptide (SP)10spasmolytic protein 1; SP; Spasmolysin; Spasmolytic Polypeptide; TFF2; trefoil factor 2

Citations for Human TFF2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Parasitic helminth infections in humans modulate Trefoil Factor levels in a manner dependent on the species of parasite and age of the host
    Authors: B Adewale, JR Heintz, CF Pastore, HL Rossi, LY Hung, N Rahman, J Bethony, D Diemert, JA Babatunde, DR Herbert
    PloS Neglected Tropical Diseases, 2021;15(10):e0009550.
    Species: Human
    Sample Types: Serum
  2. Trefoil factor(s) and CA19.9: A promising panel for early detection of pancreatic cancer
    Authors: R Jahan, K Ganguly, LM Smith, P Atri, J Carmicheal, Y Sheinin, S Rachagani, G Natarajan, RE Brand, MA Macha, PM Grandgenet, S Kaur, SK Batra
    EBioMedicine, 2019;0(0):.
    Species: Human
    Sample Types: Serum
  3. Increased trefoil factor 2 levels in patients with chronic kidney disease
    Authors: D Lebherz-Ei, B Tudor, HJ Ankersmit, T Reiter, M Haas, E Einwallner, F Roth-Walte, CG Krenn, GA Roth
    PLoS ONE, 2017;12(3):e0174551.
    Species: Human
    Sample Types: Serum


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