Home / Thioredoxin-like 5/TRP14 / Human Thioredoxin-like 5/TRP14 Antibody

Human Thioredoxin-like 5/TRP14 Antibody

Catalog #: MAB3504
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3504
MAB3504-SP

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Detection of Human Thioredoxin-like 5/TRP14 by Western Blot.
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Human Thioredoxin-like 5/TRP14 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human TRP14 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 544710
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TRP14
Ala2-Asp123
Accession # Q9BRA2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Thioredoxin-like 5/TRP14 antibody by Western Blot. View Larger

Detection of Human Thioredoxin-like 5/TRP14 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat acute T cell leukemia cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Thioredoxin-like 5/TRP14 Monoclonal Antibody (Catalog # MAB3504) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin-like 5/TRP14 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thioredoxin-like 5/TRP14

Thioredoxin-like 5, also known as a 14 kDa thioredoxin-related protein (TRP14) is a cytosolic redox regulatory molecule that belongs to the thioredoxin family of proteins. It contains a thioredoxin-like redox-sensitive active site motif (CPDC). In its reduced form, the active site cysteine residues reduce protein disulfide. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by the NADPH-dependent thioredoxin reductase 1. Thioredoxin-like 5 is ubiquitously expressed and acts on protein targets different from that of Thioredoxin-1. The amino acid sequence of human Thioredoxin-like 5 is 80% identical to that of mouse and rat Thioredoxin-like 5.

Entrez Gene IDs
84817 (Human); 52700 (Mouse)
Alternate Names
MGC14353; Protein 42-9-9; thioredoxin (Trx)-related protein, 14 kDa; thioredoxin domain containing 17; thioredoxin domain-containing protein 17; Thioredoxin like 5; Thioredoxin-like 5; Thioredoxin-like protein 5; TRP14; TRP1414 kDa thioredoxin-related protein; TXNDC17; TXNL5

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Citation for Human Thioredoxin-like 5/TRP14 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A novel persulfide detection method reveals protein persulfide- and polysulfide-reducing functions of thioredoxin and glutathione systems
    Authors: Éva Dóka, Irina Pader, Adrienn Bíró, Katarina Johansson, Qing Cheng, Krisztina Ballagó et al.
    Science Advances

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