Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 544710
View all Thioredoxin-like 5/TRP14 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TRP14
Ala2-Asp123
Accession # Q9BRA2

Specificity

Detects endogenous human TRP14 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Thioredoxin-like 5/TRP14 Antibody

Detection of Human Thioredoxin-like 5/TRP14 antibody by Western Blot.

Detection of Human Thioredoxin-like 5/TRP14 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat acute T cell leukemia cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Thioredoxin-like 5/TRP14 Monoclonal Antibody (Catalog # MAB3504) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin-like 5/TRP14 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human Thioredoxin-like 5/TRP14 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Jurkat acute T cell leukemia cell line, and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thioredoxin-like 5/TRP14

Thioredoxin-like 5, also known as a 14 kDa thioredoxin-related protein (TRP14) is a cytosolic redox regulatory molecule that belongs to the thioredoxin family of proteins. It contains a thioredoxin-like redox-sensitive active site motif (CPDC). In its reduced form, the active site cysteine residues reduce protein disulfide. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by the NADPH-dependent thioredoxin reductase 1. Thioredoxin-like 5 is ubiquitously expressed and acts on protein targets different from that of Thioredoxin-1. The amino acid sequence of human Thioredoxin-like 5 is 80% identical to that of mouse and rat Thioredoxin-like 5.

Alternate Names

Thioredoxin like 5, TRP14, TXNDC17, TXNL5

Entrez Gene IDs

84817 (Human); 52700 (Mouse)

Gene Symbol

TXNDC17

UniProt

Q9BRA2

Additional Thioredoxin-like 5/TRP14 Products

Product Documents for Human Thioredoxin-like 5/TRP14 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Thioredoxin-like 5/TRP14 Antibody

For research use only

Citations for Human Thioredoxin-like 5/TRP14 Antibody

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