Detection of Human Thioredoxin-like 5/TRP14 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat acute T cell leukemia cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Thioredoxin-like 5/TRP14 Monoclonal Antibody (Catalog # MAB3504) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin-like 5/TRP14 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thioredoxin-like 5/TRP14
Thioredoxin-like 5, also known as a 14 kDa thioredoxin-related protein (TRP14) is a cytosolic redox regulatory molecule that belongs to the thioredoxin family of proteins. It contains a thioredoxin-like redox-sensitive active site motif (CPDC). In its reduced form, the active site cysteine residues reduce protein disulfide. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by the NADPH-dependent thioredoxin reductase 1. Thioredoxin-like 5 is ubiquitously expressed and acts on protein targets different from that of Thioredoxin-1. The amino acid sequence of human Thioredoxin-like 5 is 80% identical to that of mouse and rat Thioredoxin-like 5.
Entrez Gene IDs:
84817 (Human); 52700 (Mouse)
MGC14353; Protein 42-9-9; thioredoxin (Trx)-related protein, 14 kDa; thioredoxin domain containing 17; thioredoxin domain-containing protein 17; Thioredoxin like 5; Thioredoxin-like 5; Thioredoxin-like protein 5; TRP14; TRP1414 kDa thioredoxin-related protein; TXNDC17; TXNL5
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