Human Thioredoxin-like 5/TRP14 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Thioredoxin-like 5/TRP14 by Western Blot.
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Product Details
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Human Thioredoxin-like 5/TRP14 Antibody Summary

Species Reactivity
Detects endogenous human TRP14 in Western blots.
Monoclonal Mouse IgG1 Clone # 544710
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human TRP14
Accession # Q9BRA2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Thioredoxin-like 5/TRP14 antibody by Western Blot. View Larger

Detection of Human Thioredoxin-like 5/TRP14 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat acute T cell leukemia cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Thioredoxin-like 5/TRP14 Monoclonal Antibody (Catalog # MAB3504) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin-like 5/TRP14 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thioredoxin-like 5/TRP14

Thioredoxin-like 5, also known as a 14 kDa thioredoxin-related protein (TRP14) is a cytosolic redox regulatory molecule that belongs to the thioredoxin family of proteins. It contains a thioredoxin-like redox-sensitive active site motif (CPDC). In its reduced form, the active site cysteine residues reduce protein disulfide. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by the NADPH-dependent thioredoxin reductase 1. Thioredoxin-like 5 is ubiquitously expressed and acts on protein targets different from that of Thioredoxin-1. The amino acid sequence of human Thioredoxin-like 5 is 80% identical to that of mouse and rat Thioredoxin-like 5.

Entrez Gene IDs
84817 (Human); 52700 (Mouse)
Alternate Names
MGC14353; Protein 42-9-9; thioredoxin (Trx)-related protein, 14 kDa; thioredoxin domain containing 17; thioredoxin domain-containing protein 17; Thioredoxin like 5; Thioredoxin-like 5; Thioredoxin-like protein 5; TRP14; TRP1414 kDa thioredoxin-related protein; TXNDC17; TXNL5

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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