Human Thrombopoietin/Tpo Biotinylated Antibody

Catalog #: BAM288
1 Citation Datasheet / COA / SDS

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BAM288

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Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Thrombopoietin/Tpo Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Tpo in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) G-CSF, rhGM-CSF, rmGM-CSF, or rhM-CSF.

Source

Monoclonal Mouse IgG_2A Clone # 34840

Purification

Protein A or G purified from ascites

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tpo
Ser22-Gly353
Accession # P40225

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

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Applications

Recommended Concentration

Sample

Human Thrombopoietin/Tpo Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human Thrombopoietin/Tpo Antibody (Catalog # MAB288)

Detection Reagent: Human Thrombopoietin/Tpo Biotinylated Antibody (Catalog # BAF288)

Standard: Recombinant Human Thrombopoietin (NS0-expressed) Protein (Catalog # 288-TPN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombopoietin/Tpo

Tpo is a secreted protein that regulates megakaryocytopoiesis and thrombopoiesis. It binds to and induces signaling through the thrombopoietin receptor.

Entrez Gene IDs

7066 (Human); 21832 (Mouse); 81811 (Rat)

Alternate Names

Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MKCSF; MK-CSF; ML; MPL ligand; MPLLG; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THCYT1; THPO; thrombopoietin nirs variant 1; Thrombopoietin; Tpo; TPOMKCSF

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Thrombopoietin/Tpo Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010;56(0):1320.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development