Human Thrombopoietin/Tpo Biotinylated Antibody

R&D Systems | Catalog # BAM288

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA Detection (Matched Antibody Pair)

Cited:

Luminex Development

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2A Clone # 34840
View all Thrombopoietin/Tpo Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tpo
Ser22-Gly353
Accession # P40225

Specificity

Detects human Tpo in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) G-CSF, rhGM-CSF, rmGM-CSF, or rhM-CSF.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Thrombopoietin/Tpo Biotinylated Antibody

Human Thrombopoietin/Tpo Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human Thrombopoietin/Tpo Antibody (Catalog # MAB288)
  • Standard: Recombinant Human Thrombopoietin (NS0-expressed) Protein (Catalog # 288-TPN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombopoietin/Tpo

Tpo is a secreted protein that regulates megakaryocytopoiesis and thrombopoiesis. It binds to and induces signaling through the thrombopoietin receptor.

Alternate Names

MGDF, MK-CSF, MKCSF, MPLLG, THCYT1, THPO, Tpo

Entrez Gene IDs

7066 (Human); 21832 (Mouse); 81811 (Rat)

Gene Symbol

THPO

UniProt

P40225

Additional Thrombopoietin/Tpo Products

Product Documents for Human Thrombopoietin/Tpo Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Thrombopoietin/Tpo Biotinylated Antibody

For research use only

Citations for Human Thrombopoietin/Tpo Biotinylated Antibody

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