Detects human Thrombopoietin/Tpo in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) G-CSF, rhG-CSF R, rhGM-CSF, recombinant mouse GM-CSF, or rhM-CSF is observed.
Monoclonal Mouse IgG2B Clone # 34817
Protein A or G purified from ascites
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Thrombopoietin/Tpo Ser22-Gly353 Accession # P40225
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Human Thrombopoietin/Tpo Antibody (Catalog #
ELISA Detection (Matched Antibody Pair)
Human Thrombopoietin/Tpo Biotinylated Antibody (Catalog #
Recombinant Human Thrombopoietin (NS0-expressed) Protein (Catalog #
Measured by its ability to neutralize Thrombopoietin/Tpo-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 5‑10 µg/mL in the presence of 1.5 ng/mL Recombinant Human Thrombopoietin/Tpo.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Cell Proliferation Induced by Thrombopoietin and Neutralization by Human Thrombopoietin Antibody.
Recombinant Human Thrombopoietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombopoietin/Tpo (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB288). The ND50 is typically 5‑10 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Tpo is a secreted protein that regulates megakaryocytopoiesis and
thrombopoiesis. It binds to and induces signaling through the Tpo
receptor, which is encoded by the c-Mpl protooncogene.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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