Human Thrombopoietin/Tpo Antibody

Catalog # Availability Size / Price Qty
Cell Proliferation Induced by Thrombopoietin and Neutralization by Human Thrombopoietin Antibody.
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Product Details
Citations (1)
Supplemental Products

Human Thrombopoietin/Tpo Antibody Summary

Species Reactivity
Detects human Thrombopoietin/Tpo in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) G-CSF, rhG-CSF R, rhGM-CSF, recombinant mouse GM-CSF, or rhM-CSF is observed.
Monoclonal Mouse IgG2B Clone # 34817
Protein A or G purified from ascites
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Thrombopoietin/Tpo
Accession # P40225
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

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Recommended Concentration

Human Thrombopoietin/Tpo Sandwich Immunoassay

Recommended Concentration
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Thrombopoietin/Tpo Biotinylated Antibody (Catalog # BAF288)

Detection Reagent: Human Thrombopoietin/Tpo Biotinylated Antibody (Catalog # BAM288)

Standard: Recombinant Human Thrombopoietin (NS0-expressed) Protein (Catalog # 288-TPN)

Measured by its ability to neutralize Thrombopoietin/Tpo-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 5‑10 µg/mL in the presence of 1.5 ng/mL Recombinant Human Thrombopoietin/Tpo.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by Thrombopoietin and Neutralization by Human Thrombopoietin Antibody. View Larger

Cell Proliferation Induced by Thrombopoietin and Neutralization by Human Thrombopoietin Antibody. Recombinant Human Thrombo-poietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human mega-karyocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombo-poietin/Tpo (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Thrombo-poietin/Tpo Monoclonal Antibody (Catalog # MAB288). The ND50 is typically 5-10 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombopoietin/Tpo

Tpo is a secreted protein that regulates megakaryocytopoiesis and thrombopoiesis. It binds to and induces signaling through the Tpo receptor, which is encoded by the c-Mpl protooncogene.

Entrez Gene IDs
7066 (Human); 21832 (Mouse); 81811 (Rat)
Alternate Names
Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MKCSF; MK-CSF; ML; MPL ligand; MPLLG; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THCYT1; THPO; thrombopoietin nirs variant 1; Thrombopoietin; Tpo; TPOMKCSF

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Citation for Human Thrombopoietin/Tpo Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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