Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Detection, Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all TIMP-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TIMP-1 (R&D Systems, Catalog # 970-TM)
Cys24-Ala207
Accession # Q6FGX5

Specificity

Detects human TIMP-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with rmTIMP-1 and rhTIMP-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human TIMP‑1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TIMP‑1 (Catalog # 970-TM)

Human TIMP-1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human TIMP‑1 Antibody (Catalog # MAB970)
  • Standard: Recombinant Human TIMP-1 Protein, CF (Catalog # 970-TM)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using BAF970 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIMP-1

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Alternate Names

TIMP1

Entrez Gene IDs

7076 (Human); 21857 (Mouse); 116510 (Rat)

Gene Symbol

TIMP1

UniProt

Q6FGX5

Additional TIMP-1 Products

Product Documents for Human TIMP‑1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TIMP‑1 Biotinylated Antibody

For research use only

Citations for Human TIMP‑1 Biotinylated Antibody

Customer Reviews for Human TIMP‑1 Biotinylated Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Human TIMP-1 Biotinylated Antibody
    Name: Hector Palacios
    Application: Immunoprecipitation
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/12/2018
  • Human TIMP-1 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/16/2017
    This antibody was used as the detection molecule in a sandwich ELISA targeting TIMP-1. The antibody MAB970 was used as the capture molecule.
    Human TIMP‑1 Biotinylated Antibody BAF970

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