Human TIMP-1 Antibody

  (7 citations)
(1 Review)
    
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Product Details
Citations (7)
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  • Species Reactivity
    Human
  • Specificity
    Detects human TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse TIMP‑1 and recombinant rat TIMP-1 is observed and less than 1% cross-reactivity with recombinant human (rh) TIMP-2, rhTIMP-3, and rhTIMP‑4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TIMP-1
    Cys24-Ala207
    Accession # Q6FGX5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1-5 µg/mL
    See below
  • Simple Western
    50 µg/mL
    See below
  • Knockout Validated
    TIMP‑1 is specifically detected in SK‑OV‑3 human ovarian adenocarcinoma parental cell line but is not detectable in TIMP‑1 knockout SK‑OV‑3 cell line.
  • Neutralization
    Measured by its ability to neutralize Recombinant Human TIMP-1 (0.1 µg/mL, Catalog # 970-TM) inhibition of Recombinant Human MMP‑2 (0.2 µg/mL, Catalog # 902-MP) cleavage of the fluorogenic peptide substrate Mca‑PLGL-Dpa-AR-NH2 (5 µM, Catalog # ES001). The Neutralization Dose (ND50) is typically 1 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TIMP‑1 by Western Blot. Western blot shows lysates of human lung tissue and human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP‑1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human TIMP‑1 by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, and SK‑OV‑3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP‑1 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human TIMP‑1 by Simple WesternTM. Simple Western lane view shows lysates of PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, and human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for TIMP‑1 at approximately 42-45 kDa (as indicated) using 50 µg/mL of Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.
Neutralization of TIMP‑1 Activity by Human TIMP‑1 Antibody. Recombinant Human MMP‑2 (0.2 µg/mL, Catalog # 902-MP) activity is measured in the presence of Recombinant Human TIMP-1 (0.1 µg/mL, Catalog # 970-TM) that has been preincubated with increasing concentrations of Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970). The ND50 is typically 1 µg/mL.
Knockout Validated
Western Blot Shows Human TIMP‑1 Specificity by Using Knockout Cell Line. Western blot shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line and TIMP-1 knockout SK-OV-3 cell line (KO). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP‑1 at approximately 25 kDa (as indicated) in the parental SK‑OV‑3 cell line, but is not detectable in knockout SK‑OV‑3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-1

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.

  • Long Name:
    Tissue Inhibitors of Metalloproteinases 1
  • Entrez Gene IDs:
    7076 (Human); 21857 (Mouse); 116510 (Rat)
  • Alternate Names:
    CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Targeting TR4 nuclear receptor suppresses prostate cancer invasion via reduction of infiltrating macrophages with alteration of the TIMP-1/MMP2/MMP9 signals.
    Authors: Ding X, Yang D, Xia L, Chen B, Yu S, Niu Y, Wang M, Li G, Chang C
    Mol Cancer, 2015;14(0):16.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective study.
    Authors: Thorsen S, Christensen S, Wurtz S, Lundberg M, Nielsen B, Vinther L, Knowles M, Gee N, Fredriksson S, Moller S, Brunner N, Schrohl A, Stenvang J
    BMC Cancer, 2013;13(0):598.
    Species: Human
    Sample Type: Plasma
    Application: Proximity Ligation Assay
  3. Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesis.
    Authors: Tyan SW, Kuo WH, Huang CK
    PLoS ONE, 2011;6(1):e15313.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
    Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E
    Nat. Med., 2011;17(11):1498-503.
    Species: Human
    Sample Type: In Vivo
    Application: In Vivo Homing
  5. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2010;84(3):239-51.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Fibroblast-conditioned media promote human sarcoma cell invasion.
    Authors: Bittner JG, Wilson M, Shah MB, Albo D, Feig BW, Wang TN
    Surgery, 2009;145(1):42-7.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
    Authors: Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE
    J. Cell Biol., 2006;175(1):179-91.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 203  
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB , Simple Western HAF109 20  
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Goat IgG HRP-conjugated Antibody

WB HAF017 16  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL003 6
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 7  
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL002
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB , ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ELISA.

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  Excellent
 ELISA Human Anonymous 12/06/2017
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Application ELISA
Sample Tested EDTA Plasma
Species Human

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